Gordonia phage CarolAnn
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B3AZ59|A0A1B3AZ59_9CAUD Uncharacterized protein OS=Gordonia phage CarolAnn OX=1887644 GN=43 PE=4 SV=1
MM1 pKa = 7.46 ANDD4 pKa = 4.61 TIFEE8 pKa = 4.28 LPEE11 pKa = 4.65 IPGVTFTASYY21 pKa = 10.84 GSGGEE26 pKa = 4.12 TGLPSNWIRR35 pKa = 11.84 IVGTVEE41 pKa = 3.86 NPWYY45 pKa = 10.31 DD46 pKa = 3.26 PTYY49 pKa = 11.17 NYY51 pKa = 11.17 GLDD54 pKa = 4.0 PNGHH58 pKa = 5.86 TEE60 pKa = 4.02 MTDD63 pKa = 2.47 PWKK66 pKa = 10.71 RR67 pKa = 11.84 HH68 pKa = 4.03 TQFPTVLGPMGFEE81 pKa = 4.55 GPSIGLPTDD90 pKa = 3.96 PPPPPVEE97 pKa = 4.3 PEE99 pKa = 3.82 PTPDD103 pKa = 3.75 LEE105 pKa = 4.4 EE106 pKa = 4.25 PTVGG110 pKa = 4.31
Molecular weight: 11.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.79
IPC2_protein 3.961
IPC_protein 3.846
Toseland 3.681
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.694
Grimsley 3.592
Solomon 3.795
Lehninger 3.745
Nozaki 3.948
DTASelect 4.075
Thurlkill 3.719
EMBOSS 3.732
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A1B3AZ60|A0A1B3AZ60_9CAUD Portal protein OS=Gordonia phage CarolAnn OX=1887644 GN=3 PE=4 SV=1
MM1 pKa = 6.9 IRR3 pKa = 11.84 ARR5 pKa = 11.84 KK6 pKa = 9.97 RR7 pKa = 11.84 IDD9 pKa = 3.31 QFVLSLSFGLARR21 pKa = 11.84 PIDD24 pKa = 3.83 DD25 pKa = 4.17 RR26 pKa = 11.84 VTKK29 pKa = 10.12 TRR31 pKa = 11.84 FFFAFVLPAFVCGIAIGARR50 pKa = 11.84 LAVPAAQFLSGMSIVSGVLLALCTLSYY77 pKa = 11.01 TRR79 pKa = 11.84 VKK81 pKa = 10.84 DD82 pKa = 3.49 LAGEE86 pKa = 4.33 KK87 pKa = 9.61 GWQGADD93 pKa = 3.3 PMVPAYY99 pKa = 10.32 RR100 pKa = 11.84 FARR103 pKa = 11.84 GALTATYY110 pKa = 10.33 ISLTATALLIAQLFTGEE127 pKa = 4.07 GRR129 pKa = 11.84 PTEE132 pKa = 4.25 ALSGIALGLVVHH144 pKa = 6.52 LGVRR148 pKa = 11.84 IWTMLSAIRR157 pKa = 11.84 FQVDD161 pKa = 2.97 ATAGQRR167 pKa = 11.84 ASGPPKK173 pKa = 10.07 LRR175 pKa = 11.84 QVSS178 pKa = 3.44
Molecular weight: 19.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.794
IPC_protein 10.891
Toseland 10.979
ProMoST 11.023
Dawson 11.038
Bjellqvist 10.862
Wikipedia 11.345
Rodwell 11.052
Grimsley 11.082
Solomon 11.301
Lehninger 11.242
Nozaki 10.965
DTASelect 10.847
Thurlkill 10.965
EMBOSS 11.403
Sillero 10.994
Patrickios 10.804
IPC_peptide 11.301
IPC2_peptide 10.16
IPC2.peptide.svr19 8.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
17543
37
1863
219.3
23.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.589 ± 0.433
0.838 ± 0.131
6.937 ± 0.312
5.649 ± 0.352
2.822 ± 0.145
8.402 ± 0.361
2.206 ± 0.164
4.452 ± 0.149
3.238 ± 0.227
7.712 ± 0.26
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.167
2.799 ± 0.156
5.837 ± 0.287
3.426 ± 0.144
7.684 ± 0.409
5.284 ± 0.176
6.926 ± 0.249
7.918 ± 0.267
2.018 ± 0.112
2.121 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here