Lemur associated porprismacovirus 1
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5GP14|A0A0B5GP14_9VIRU Rep protein OS=Lemur associated porprismacovirus 1 OX=2170115 GN=rep PE=4 SV=1
MM1 pKa = 7.83 RR2 pKa = 11.84 VQISEE7 pKa = 4.39 TYY9 pKa = 10.65 DD10 pKa = 3.21 LSTKK14 pKa = 8.92 INKK17 pKa = 8.59 MGIVGIHH24 pKa = 5.65 TPVGPLLNRR33 pKa = 11.84 LYY35 pKa = 10.35 PGLVLNYY42 pKa = 10.33 KK43 pKa = 9.97 KK44 pKa = 10.69 FRR46 pKa = 11.84 FSKK49 pKa = 10.72 CDD51 pKa = 2.92 ITLACASMLPADD63 pKa = 4.27 PLQVGVEE70 pKa = 4.24 AGAIAPQDD78 pKa = 3.66 MFNPILFKK86 pKa = 10.81 AVSNDD91 pKa = 2.84 TMNQFVNYY99 pKa = 7.83 IQYY102 pKa = 10.69 YY103 pKa = 7.96 GASNKK108 pKa = 8.52 TLALDD113 pKa = 4.25 GSSVVAVNDD122 pKa = 3.71 AVFKK126 pKa = 11.03 AGSTQATDD134 pKa = 3.34 QFSLYY139 pKa = 10.36 YY140 pKa = 10.58 SLLADD145 pKa = 3.51 PKK147 pKa = 9.26 GWRR150 pKa = 11.84 KK151 pKa = 9.97 AMPQSGLQMRR161 pKa = 11.84 NLRR164 pKa = 11.84 PLVFQVVEE172 pKa = 4.5 NGAPLQGRR180 pKa = 11.84 VGVADD185 pKa = 4.35 PATGDD190 pKa = 3.46 INSGAFIGDD199 pKa = 3.77 PASGQSYY206 pKa = 8.62 TGEE209 pKa = 4.12 SWGVPSDD216 pKa = 3.64 ASGTAVLSSNKK227 pKa = 9.52 GVNIYY232 pKa = 10.1 RR233 pKa = 11.84 GRR235 pKa = 11.84 AIRR238 pKa = 11.84 MPWISTKK245 pKa = 10.25 FFNNGTGVADD255 pKa = 4.45 SNTIMPSFAQIVEE268 pKa = 4.27 GTNVSTNTGRR278 pKa = 11.84 VPPTYY283 pKa = 9.19 VACIIVPPAKK293 pKa = 10.61 LNVLYY298 pKa = 11.08 YY299 pKa = 10.4 RR300 pKa = 11.84 MKK302 pKa = 9.73 VTWTVEE308 pKa = 4.08 FTGLSSMTPYY318 pKa = 10.84 LGWNDD323 pKa = 3.61 LTDD326 pKa = 3.85 TADD329 pKa = 2.99 IAYY332 pKa = 7.57 GTDD335 pKa = 3.31 YY336 pKa = 11.66 ANQASVAMTSLEE348 pKa = 4.24 TMVDD352 pKa = 3.26 TSEE355 pKa = 4.21 VDD357 pKa = 2.94 MSKK360 pKa = 10.44 IMEE363 pKa = 4.23 GG364 pKa = 3.32
Molecular weight: 39.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.233
IPC2_protein 5.525
IPC_protein 5.41
Toseland 5.308
ProMoST 5.575
Dawson 5.423
Bjellqvist 5.537
Wikipedia 5.347
Rodwell 5.308
Grimsley 5.232
Solomon 5.423
Lehninger 5.385
Nozaki 5.55
DTASelect 5.779
Thurlkill 5.359
EMBOSS 5.397
Sillero 5.601
Patrickios 3.834
IPC_peptide 5.423
IPC2_peptide 5.588
IPC2.peptide.svr19 5.693
Protein with the highest isoelectric point:
>tr|A0A0B5GP14|A0A0B5GP14_9VIRU Rep protein OS=Lemur associated porprismacovirus 1 OX=2170115 GN=rep PE=4 SV=1
MM1 pKa = 7.21 TNVYY5 pKa = 9.44 MITMPRR11 pKa = 11.84 KK12 pKa = 9.07 GYY14 pKa = 10.4 SGFIAIMRR22 pKa = 11.84 FFRR25 pKa = 11.84 KK26 pKa = 9.61 FDD28 pKa = 3.26 VHH30 pKa = 6.97 KK31 pKa = 10.34 WIVSPEE37 pKa = 3.9 KK38 pKa = 10.01 GAQGYY43 pKa = 7.27 EE44 pKa = 3.37 HH45 pKa = 6.01 WQIRR49 pKa = 11.84 FRR51 pKa = 11.84 CGLTPEE57 pKa = 4.2 NAMIAWRR64 pKa = 11.84 QWVCNGFNMLEE75 pKa = 4.09 ASDD78 pKa = 4.79 NGWEE82 pKa = 4.08 YY83 pKa = 10.96 EE84 pKa = 4.13 GKK86 pKa = 9.97 EE87 pKa = 4.29 GKK89 pKa = 9.86 FLASWDD95 pKa = 3.92 SKK97 pKa = 9.87 GARR100 pKa = 11.84 SVRR103 pKa = 11.84 FGKK106 pKa = 9.07 MEE108 pKa = 3.63 WRR110 pKa = 11.84 QEE112 pKa = 4.02 ATVLRR117 pKa = 11.84 ARR119 pKa = 11.84 ATNDD123 pKa = 2.97 RR124 pKa = 11.84 EE125 pKa = 4.06 IVVWYY130 pKa = 9.72 DD131 pKa = 3.16 PKK133 pKa = 11.42 GNSGKK138 pKa = 9.99 SWLVGHH144 pKa = 6.86 LVEE147 pKa = 4.86 TRR149 pKa = 11.84 QAYY152 pKa = 7.69 YY153 pKa = 10.42 VPPYY157 pKa = 9.89 LATVEE162 pKa = 4.82 SMIKK166 pKa = 9.11 TLASMVKK173 pKa = 10.14 ADD175 pKa = 4.83 RR176 pKa = 11.84 EE177 pKa = 4.32 NGNPPRR183 pKa = 11.84 PLVVIDD189 pKa = 3.94 IPRR192 pKa = 11.84 SYY194 pKa = 10.36 KK195 pKa = 9.16 WKK197 pKa = 9.38 NDD199 pKa = 3.0 MYY201 pKa = 11.33 VAIEE205 pKa = 4.42 AIKK208 pKa = 10.62 DD209 pKa = 3.88 GIIVDD214 pKa = 3.85 PRR216 pKa = 11.84 YY217 pKa = 10.12 SATVEE222 pKa = 3.95 NVKK225 pKa = 10.88 GIGVIVITNEE235 pKa = 3.49 KK236 pKa = 8.57 PQVGKK241 pKa = 10.76 LSADD245 pKa = 2.98 RR246 pKa = 11.84 WDD248 pKa = 3.48 IVDD251 pKa = 3.32 WW252 pKa = 4.39
Molecular weight: 29.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.718
IPC2_protein 8.668
IPC_protein 8.624
Toseland 9.224
ProMoST 9.048
Dawson 9.531
Bjellqvist 9.282
Wikipedia 9.721
Rodwell 9.736
Grimsley 9.619
Solomon 9.589
Lehninger 9.545
Nozaki 9.326
DTASelect 9.238
Thurlkill 9.37
EMBOSS 9.663
Sillero 9.487
Patrickios 4.8
IPC_peptide 9.575
IPC2_peptide 7.863
IPC2.peptide.svr19 7.735
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
616
252
364
308.0
34.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.117 ± 0.584
0.812 ± 0.011
5.357 ± 0.119
4.058 ± 1.375
3.409 ± 0.141
8.117 ± 0.346
0.649 ± 0.325
6.006 ± 0.682
5.357 ± 1.072
5.844 ± 1.126
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.058 ± 0.054
5.519 ± 0.455
5.357 ± 0.357
3.247 ± 0.52
4.87 ± 1.364
6.981 ± 1.093
6.169 ± 1.32
8.929 ± 0.119
2.76 ± 1.201
4.383 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here