Amino acid dipepetide frequency for Simplicispira sp. 125

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.239AlaAla: 17.239 ± 0.188
1.392AlaCys: 1.392 ± 0.039
5.759AlaAsp: 5.759 ± 0.071
6.117AlaGlu: 6.117 ± 0.071
3.96AlaPhe: 3.96 ± 0.062
9.917AlaGly: 9.917 ± 0.1
3.08AlaHis: 3.08 ± 0.051
5.154AlaIle: 5.154 ± 0.076
3.589AlaLys: 3.589 ± 0.082
16.128AlaLeu: 16.128 ± 0.152
3.603AlaMet: 3.603 ± 0.065
2.734AlaAsn: 2.734 ± 0.052
6.61AlaPro: 6.61 ± 0.107
7.307AlaGln: 7.307 ± 0.11
8.905AlaArg: 8.905 ± 0.1
6.755AlaSer: 6.755 ± 0.085
6.333AlaThr: 6.333 ± 0.091
9.273AlaVal: 9.273 ± 0.097
2.035AlaTrp: 2.035 ± 0.044
2.361AlaTyr: 2.361 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.249CysAla: 1.249 ± 0.037
0.129CysCys: 0.129 ± 0.011
0.506CysAsp: 0.506 ± 0.023
0.513CysGlu: 0.513 ± 0.02
0.32CysPhe: 0.32 ± 0.014
0.95CysGly: 0.95 ± 0.03
0.286CysHis: 0.286 ± 0.017
0.449CysIle: 0.449 ± 0.019
0.263CysLys: 0.263 ± 0.018
0.875CysLeu: 0.875 ± 0.028
0.246CysMet: 0.246 ± 0.012
0.268CysAsn: 0.268 ± 0.015
0.536CysPro: 0.536 ± 0.024
0.311CysGln: 0.311 ± 0.016
0.533CysArg: 0.533 ± 0.022
0.515CysSer: 0.515 ± 0.019
0.595CysThr: 0.595 ± 0.023
0.711CysVal: 0.711 ± 0.025
0.126CysTrp: 0.126 ± 0.011
0.205CysTyr: 0.205 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.984AspAla: 6.984 ± 0.088
0.483AspCys: 0.483 ± 0.022
2.483AspAsp: 2.483 ± 0.055
2.891AspGlu: 2.891 ± 0.051
1.968AspPhe: 1.968 ± 0.042
4.209AspGly: 4.209 ± 0.065
1.129AspHis: 1.129 ± 0.034
2.45AspIle: 2.45 ± 0.05
1.775AspLys: 1.775 ± 0.045
5.328AspLeu: 5.328 ± 0.064
1.331AspMet: 1.331 ± 0.033
1.152AspAsn: 1.152 ± 0.031
2.713AspPro: 2.713 ± 0.047
1.711AspGln: 1.711 ± 0.042
3.04AspArg: 3.04 ± 0.055
2.21AspSer: 2.21 ± 0.048
2.586AspThr: 2.586 ± 0.048
3.78AspVal: 3.78 ± 0.059
0.917AspTrp: 0.917 ± 0.03
1.306AspTyr: 1.306 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
6.335GluAla: 6.335 ± 0.082
0.419GluCys: 0.419 ± 0.02
2.192GluAsp: 2.192 ± 0.048
2.477GluGlu: 2.477 ± 0.055
1.713GluPhe: 1.713 ± 0.04
3.759GluGly: 3.759 ± 0.058
1.344GluHis: 1.344 ± 0.039
2.586GluIle: 2.586 ± 0.053
2.019GluLys: 2.019 ± 0.045
5.445GluLeu: 5.445 ± 0.074
1.358GluMet: 1.358 ± 0.034
1.353GluAsn: 1.353 ± 0.035
2.424GluPro: 2.424 ± 0.047
2.767GluGln: 2.767 ± 0.051
4.518GluArg: 4.518 ± 0.071
2.546GluSer: 2.546 ± 0.05
2.543GluThr: 2.543 ± 0.04
3.988GluVal: 3.988 ± 0.064
0.682GluTrp: 0.682 ± 0.022
1.005GluTyr: 1.005 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.147PheAla: 4.147 ± 0.068
0.379PheCys: 0.379 ± 0.018
2.418PheAsp: 2.418 ± 0.044
1.976PheGlu: 1.976 ± 0.041
1.356PhePhe: 1.356 ± 0.033
3.224PheGly: 3.224 ± 0.057
0.753PheHis: 0.753 ± 0.03
1.546PheIle: 1.546 ± 0.038
1.16PheLys: 1.16 ± 0.037
2.975PheLeu: 2.975 ± 0.06
0.855PheMet: 0.855 ± 0.024
1.098PheAsn: 1.098 ± 0.03
1.437PhePro: 1.437 ± 0.039
1.116PheGln: 1.116 ± 0.031
1.689PheArg: 1.689 ± 0.041
2.177PheSer: 2.177 ± 0.043
1.893PheThr: 1.893 ± 0.05
2.709PheVal: 2.709 ± 0.059
0.551PheTrp: 0.551 ± 0.022
0.862PheTyr: 0.862 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
9.358GlyAla: 9.358 ± 0.104
0.923GlyCys: 0.923 ± 0.033
3.865GlyAsp: 3.865 ± 0.053
4.176GlyGlu: 4.176 ± 0.071
3.148GlyPhe: 3.148 ± 0.057
6.808GlyGly: 6.808 ± 0.105
2.029GlyHis: 2.029 ± 0.045
3.865GlyIle: 3.865 ± 0.06
3.339GlyLys: 3.339 ± 0.06
8.932GlyLeu: 8.932 ± 0.096
2.607GlyMet: 2.607 ± 0.043
2.084GlyAsn: 2.084 ± 0.046
2.931GlyPro: 2.931 ± 0.053
3.685GlyGln: 3.685 ± 0.06
5.159GlyArg: 5.159 ± 0.074
4.591GlySer: 4.591 ± 0.061
4.4GlyThr: 4.4 ± 0.072
6.703GlyVal: 6.703 ± 0.084
1.425GlyTrp: 1.425 ± 0.038
2.233GlyTyr: 2.233 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
3.069HisAla: 3.069 ± 0.052
0.323HisCys: 0.323 ± 0.016
1.223HisAsp: 1.223 ± 0.039
1.144HisGlu: 1.144 ± 0.033
0.95HisPhe: 0.95 ± 0.029
2.125HisGly: 2.125 ± 0.045
0.68HisHis: 0.68 ± 0.027
1.086HisIle: 1.086 ± 0.032
0.645HisLys: 0.645 ± 0.024
2.4HisLeu: 2.4 ± 0.047
0.64HisMet: 0.64 ± 0.023
0.566HisAsn: 0.566 ± 0.022
1.578HisPro: 1.578 ± 0.039
0.94HisGln: 0.94 ± 0.03
1.487HisArg: 1.487 ± 0.037
1.259HisSer: 1.259 ± 0.036
1.272HisThr: 1.272 ± 0.034
1.544HisVal: 1.544 ± 0.036
0.457HisTrp: 0.457 ± 0.019
0.643HisTyr: 0.643 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
5.939IleAla: 5.939 ± 0.077
0.383IleCys: 0.383 ± 0.019
2.974IleAsp: 2.974 ± 0.053
2.977IleGlu: 2.977 ± 0.057
1.348IlePhe: 1.348 ± 0.036
3.785IleGly: 3.785 ± 0.065
0.887IleHis: 0.887 ± 0.031
1.579IleIle: 1.579 ± 0.046
1.55IleLys: 1.55 ± 0.046
3.301IleLeu: 3.301 ± 0.064
0.802IleMet: 0.802 ± 0.029
1.397IleAsn: 1.397 ± 0.04
2.012IlePro: 2.012 ± 0.042
1.302IleGln: 1.302 ± 0.036
2.349IleArg: 2.349 ± 0.049
2.384IleSer: 2.384 ± 0.048
2.642IleThr: 2.642 ± 0.051
3.395IleVal: 3.395 ± 0.058
0.439IleTrp: 0.439 ± 0.021
0.982IleTyr: 0.982 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
4.05LysAla: 4.05 ± 0.087
0.154LysCys: 0.154 ± 0.01
1.695LysAsp: 1.695 ± 0.045
1.595LysGlu: 1.595 ± 0.041
0.886LysPhe: 0.886 ± 0.035
2.535LysGly: 2.535 ± 0.054
0.667LysHis: 0.667 ± 0.022
1.519LysIle: 1.519 ± 0.04
1.526LysLys: 1.526 ± 0.057
3.206LysLeu: 3.206 ± 0.064
0.902LysMet: 0.902 ± 0.032
1.058LysAsn: 1.058 ± 0.031
1.878LysPro: 1.878 ± 0.042
1.185LysGln: 1.185 ± 0.03
2.012LysArg: 2.012 ± 0.052
1.714LysSer: 1.714 ± 0.038
2.066LysThr: 2.066 ± 0.051
2.439LysVal: 2.439 ± 0.061
0.322LysTrp: 0.322 ± 0.017
0.655LysTyr: 0.655 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
15.193LeuAla: 15.193 ± 0.158
1.127LeuCys: 1.127 ± 0.032
5.707LeuAsp: 5.707 ± 0.079
5.356LeuGlu: 5.356 ± 0.078
3.368LeuPhe: 3.368 ± 0.063
8.844LeuGly: 8.844 ± 0.111
2.67LeuHis: 2.67 ± 0.055
4.043LeuIle: 4.043 ± 0.068
3.335LeuLys: 3.335 ± 0.061
11.806LeuLeu: 11.806 ± 0.159
2.592LeuMet: 2.592 ± 0.043
2.634LeuAsn: 2.634 ± 0.044
6.238LeuPro: 6.238 ± 0.088
5.363LeuGln: 5.363 ± 0.072
7.866LeuArg: 7.866 ± 0.104
5.961LeuSer: 5.961 ± 0.081
5.318LeuThr: 5.318 ± 0.079
8.153LeuVal: 8.153 ± 0.103
1.495LeuTrp: 1.495 ± 0.046
2.056LeuTyr: 2.056 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.511MetAla: 3.511 ± 0.069
0.172MetCys: 0.172 ± 0.013
1.262MetAsp: 1.262 ± 0.029
1.261MetGlu: 1.261 ± 0.026
0.733MetPhe: 0.733 ± 0.025
2.293MetGly: 2.293 ± 0.042
0.651MetHis: 0.651 ± 0.024
0.856MetIle: 0.856 ± 0.028
1.009MetLys: 1.009 ± 0.034
2.789MetLeu: 2.789 ± 0.052
0.583MetMet: 0.583 ± 0.024
0.857MetAsn: 0.857 ± 0.029
1.571MetPro: 1.571 ± 0.038
1.338MetGln: 1.338 ± 0.035
1.645MetArg: 1.645 ± 0.036
1.418MetSer: 1.418 ± 0.034
1.524MetThr: 1.524 ± 0.038
2.034MetVal: 2.034 ± 0.043
0.203MetTrp: 0.203 ± 0.014
0.425MetTyr: 0.425 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.39AsnAla: 3.39 ± 0.066
0.253AsnCys: 0.253 ± 0.015
1.318AsnAsp: 1.318 ± 0.039
1.173AsnGlu: 1.173 ± 0.033
0.935AsnPhe: 0.935 ± 0.035
2.121AsnGly: 2.121 ± 0.048
0.579AsnHis: 0.579 ± 0.022
1.202AsnIle: 1.202 ± 0.035
0.791AsnLys: 0.791 ± 0.028
2.581AsnLeu: 2.581 ± 0.047
0.639AsnMet: 0.639 ± 0.025
0.747AsnAsn: 0.747 ± 0.031
1.846AsnPro: 1.846 ± 0.043
0.929AsnGln: 0.929 ± 0.029
1.596AsnArg: 1.596 ± 0.04
1.084AsnSer: 1.084 ± 0.032
1.573AsnThr: 1.573 ± 0.038
1.901AsnVal: 1.901 ± 0.045
0.375AsnTrp: 0.375 ± 0.019
0.655AsnTyr: 0.655 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
6.715ProAla: 6.715 ± 0.104
0.408ProCys: 0.408 ± 0.02
2.978ProAsp: 2.978 ± 0.056
3.337ProGlu: 3.337 ± 0.055
1.679ProPhe: 1.679 ± 0.036
4.762ProGly: 4.762 ± 0.064
1.229ProHis: 1.229 ± 0.034
1.807ProIle: 1.807 ± 0.041
1.404ProLys: 1.404 ± 0.039
5.63ProLeu: 5.63 ± 0.072
1.349ProMet: 1.349 ± 0.034
1.137ProAsn: 1.137 ± 0.032
2.788ProPro: 2.788 ± 0.071
2.461ProGln: 2.461 ± 0.047
2.834ProArg: 2.834 ± 0.051
2.74ProSer: 2.74 ± 0.048
2.68ProThr: 2.68 ± 0.05
4.528ProVal: 4.528 ± 0.062
0.844ProTrp: 0.844 ± 0.027
1.099ProTyr: 1.099 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
6.733GlnAla: 6.733 ± 0.12
0.378GlnCys: 0.378 ± 0.021
2.122GlnAsp: 2.122 ± 0.049
2.048GlnGlu: 2.048 ± 0.048
1.397GlnPhe: 1.397 ± 0.033
3.736GlnGly: 3.736 ± 0.055
1.185GlnHis: 1.185 ± 0.035
1.821GlnIle: 1.821 ± 0.04
1.32GlnLys: 1.32 ± 0.032
4.613GlnLeu: 4.613 ± 0.064
1.147GlnMet: 1.147 ± 0.031
1.021GlnAsn: 1.021 ± 0.031
2.411GlnPro: 2.411 ± 0.045
2.345GlnGln: 2.345 ± 0.059
3.867GlnArg: 3.867 ± 0.072
2.284GlnSer: 2.284 ± 0.049
2.181GlnThr: 2.181 ± 0.044
3.378GlnVal: 3.378 ± 0.056
0.847GlnTrp: 0.847 ± 0.028
0.861GlnTyr: 0.861 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
7.693ArgAla: 7.693 ± 0.091
0.592ArgCys: 0.592 ± 0.024
3.24ArgAsp: 3.24 ± 0.063
3.777ArgGlu: 3.777 ± 0.054
2.685ArgPhe: 2.685 ± 0.042
4.402ArgGly: 4.402 ± 0.071
1.743ArgHis: 1.743 ± 0.043
3.368ArgIle: 3.368 ± 0.053
2.172ArgLys: 2.172 ± 0.044
7.592ArgLeu: 7.592 ± 0.101
2.037ArgMet: 2.037 ± 0.042
1.865ArgAsn: 1.865 ± 0.043
3.037ArgPro: 3.037 ± 0.056
3.157ArgGln: 3.157 ± 0.054
4.482ArgArg: 4.482 ± 0.074
3.598ArgSer: 3.598 ± 0.055
3.439ArgThr: 3.439 ± 0.053
4.728ArgVal: 4.728 ± 0.06
1.177ArgTrp: 1.177 ± 0.035
1.761ArgTyr: 1.761 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
6.577SerAla: 6.577 ± 0.085
0.432SerCys: 0.432 ± 0.019
2.572SerAsp: 2.572 ± 0.051
2.425SerGlu: 2.425 ± 0.044
1.982SerPhe: 1.982 ± 0.045
5.025SerGly: 5.025 ± 0.063
1.237SerHis: 1.237 ± 0.038
2.482SerIle: 2.482 ± 0.049
1.562SerLys: 1.562 ± 0.042
5.685SerLeu: 5.685 ± 0.079
1.442SerMet: 1.442 ± 0.04
1.416SerAsn: 1.416 ± 0.037
2.876SerPro: 2.876 ± 0.056
1.99SerGln: 1.99 ± 0.047
3.216SerArg: 3.216 ± 0.059
3.055SerSer: 3.055 ± 0.062
3.096SerThr: 3.096 ± 0.059
3.93SerVal: 3.93 ± 0.057
0.649SerTrp: 0.649 ± 0.025
1.245SerTyr: 1.245 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.432ThrAla: 6.432 ± 0.085
0.442ThrCys: 0.442 ± 0.021
2.582ThrAsp: 2.582 ± 0.046
2.548ThrGlu: 2.548 ± 0.051
1.592ThrPhe: 1.592 ± 0.039
4.957ThrGly: 4.957 ± 0.07
1.273ThrHis: 1.273 ± 0.032
2.099ThrIle: 2.099 ± 0.048
1.281ThrLys: 1.281 ± 0.04
6.456ThrLeu: 6.456 ± 0.088
1.177ThrMet: 1.177 ± 0.039
1.216ThrAsn: 1.216 ± 0.036
3.668ThrPro: 3.668 ± 0.063
2.253ThrGln: 2.253 ± 0.051
3.183ThrArg: 3.183 ± 0.052
2.734ThrSer: 2.734 ± 0.056
3.061ThrThr: 3.061 ± 0.071
4.345ThrVal: 4.345 ± 0.065
0.7ThrTrp: 0.7 ± 0.024
1.077ThrTyr: 1.077 ± 0.035
0.0ThrXaa: 0.0 ± 0.0
Val
9.579ValAla: 9.579 ± 0.099
0.743ValCys: 0.743 ± 0.023
3.787ValAsp: 3.787 ± 0.06
3.936ValGlu: 3.936 ± 0.061
2.806ValPhe: 2.806 ± 0.057
5.865ValGly: 5.865 ± 0.084
1.793ValHis: 1.793 ± 0.042
3.053ValIle: 3.053 ± 0.058
2.17ValLys: 2.17 ± 0.052
8.868ValLeu: 8.868 ± 0.105
1.848ValMet: 1.848 ± 0.037
2.045ValAsn: 2.045 ± 0.045
4.04ValPro: 4.04 ± 0.061
3.767ValGln: 3.767 ± 0.065
5.389ValArg: 5.389 ± 0.068
3.911ValSer: 3.911 ± 0.059
3.965ValThr: 3.965 ± 0.058
6.679ValVal: 6.679 ± 0.091
1.003ValTrp: 1.003 ± 0.034
1.568ValTyr: 1.568 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.501TrpAla: 1.501 ± 0.042
0.19TrpCys: 0.19 ± 0.013
0.602TrpAsp: 0.602 ± 0.024
0.59TrpGlu: 0.59 ± 0.022
0.557TrpPhe: 0.557 ± 0.023
1.003TrpGly: 1.003 ± 0.029
0.364TrpHis: 0.364 ± 0.019
0.648TrpIle: 0.648 ± 0.025
0.484TrpLys: 0.484 ± 0.022
2.141TrpLeu: 2.141 ± 0.055
0.515TrpMet: 0.515 ± 0.02
0.46TrpAsn: 0.46 ± 0.019
0.738TrpPro: 0.738 ± 0.029
0.815TrpGln: 0.815 ± 0.029
1.185TrpArg: 1.185 ± 0.033
0.782TrpSer: 0.782 ± 0.021
0.698TrpThr: 0.698 ± 0.024
1.072TrpVal: 1.072 ± 0.03
0.307TrpTrp: 0.307 ± 0.018
0.274TrpTyr: 0.274 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.56TyrAla: 2.56 ± 0.049
0.264TyrCys: 0.264 ± 0.016
1.161TyrAsp: 1.161 ± 0.039
1.156TyrGlu: 1.156 ± 0.031
0.936TyrPhe: 0.936 ± 0.026
1.944TyrGly: 1.944 ± 0.049
0.452TyrHis: 0.452 ± 0.018
0.814TyrIle: 0.814 ± 0.025
0.671TyrLys: 0.671 ± 0.024
2.373TyrLeu: 2.373 ± 0.045
0.461TyrMet: 0.461 ± 0.02
0.579TyrAsn: 0.579 ± 0.023
1.079TyrPro: 1.079 ± 0.032
0.951TyrGln: 0.951 ± 0.031
1.592TyrArg: 1.592 ± 0.038
1.174TyrSer: 1.174 ± 0.031
1.211TyrThr: 1.211 ± 0.034
1.526TyrVal: 1.526 ± 0.037
0.372TyrTrp: 0.372 ± 0.02
0.553TyrTyr: 0.553 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3567 proteins (1182683 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski