Amino acid dipepetide frequency for Mycobacterium phage Iwokeuplikedis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.281AlaAla: 12.281 ± 1.214
0.547AlaCys: 0.547 ± 0.189
5.229AlaAsp: 5.229 ± 0.451
6.809AlaGlu: 6.809 ± 0.709
3.587AlaPhe: 3.587 ± 0.446
7.661AlaGly: 7.661 ± 0.781
1.459AlaHis: 1.459 ± 0.311
4.438AlaIle: 4.438 ± 0.573
5.776AlaLys: 5.776 ± 0.633
8.755AlaLeu: 8.755 ± 0.672
3.526AlaMet: 3.526 ± 0.599
2.979AlaAsn: 2.979 ± 0.408
4.134AlaPro: 4.134 ± 0.636
3.465AlaGln: 3.465 ± 0.488
6.08AlaArg: 6.08 ± 0.709
4.742AlaSer: 4.742 ± 0.6
4.742AlaThr: 4.742 ± 0.638
7.174AlaVal: 7.174 ± 0.73
2.128AlaTrp: 2.128 ± 0.366
2.554AlaTyr: 2.554 ± 0.502
0.0AlaXaa: 0.0 ± 0.0
Cys
0.669CysAla: 0.669 ± 0.19
0.061CysCys: 0.061 ± 0.065
0.608CysAsp: 0.608 ± 0.18
0.426CysGlu: 0.426 ± 0.158
0.243CysPhe: 0.243 ± 0.151
0.851CysGly: 0.851 ± 0.212
0.243CysHis: 0.243 ± 0.112
0.426CysIle: 0.426 ± 0.162
0.608CysLys: 0.608 ± 0.191
0.608CysLeu: 0.608 ± 0.216
0.122CysMet: 0.122 ± 0.086
0.365CysAsn: 0.365 ± 0.132
0.365CysPro: 0.365 ± 0.147
0.122CysGln: 0.122 ± 0.099
0.669CysArg: 0.669 ± 0.188
0.669CysSer: 0.669 ± 0.218
0.426CysThr: 0.426 ± 0.157
0.547CysVal: 0.547 ± 0.192
0.304CysTrp: 0.304 ± 0.138
0.365CysTyr: 0.365 ± 0.137
0.0CysXaa: 0.0 ± 0.0
Asp
6.141AspAla: 6.141 ± 0.618
0.973AspCys: 0.973 ± 0.295
4.195AspAsp: 4.195 ± 0.542
5.046AspGlu: 5.046 ± 0.631
2.554AspPhe: 2.554 ± 0.385
5.35AspGly: 5.35 ± 0.578
1.216AspHis: 1.216 ± 0.308
3.222AspIle: 3.222 ± 0.361
2.432AspLys: 2.432 ± 0.386
5.046AspLeu: 5.046 ± 0.548
1.824AspMet: 1.824 ± 0.279
1.824AspAsn: 1.824 ± 0.335
4.864AspPro: 4.864 ± 0.59
1.702AspGln: 1.702 ± 0.281
3.04AspArg: 3.04 ± 0.424
3.526AspSer: 3.526 ± 0.456
3.161AspThr: 3.161 ± 0.443
4.742AspVal: 4.742 ± 0.548
1.398AspTrp: 1.398 ± 0.322
2.371AspTyr: 2.371 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
7.478GluAla: 7.478 ± 0.852
0.243GluCys: 0.243 ± 0.114
5.776GluAsp: 5.776 ± 0.65
4.985GluGlu: 4.985 ± 0.629
2.554GluPhe: 2.554 ± 0.433
5.046GluGly: 5.046 ± 0.637
1.824GluHis: 1.824 ± 0.362
3.648GluIle: 3.648 ± 0.436
2.31GluLys: 2.31 ± 0.512
7.843GluLeu: 7.843 ± 0.828
2.554GluMet: 2.554 ± 0.443
1.763GluAsn: 1.763 ± 0.267
2.31GluPro: 2.31 ± 0.436
2.067GluGln: 2.067 ± 0.319
4.56GluArg: 4.56 ± 0.622
3.587GluSer: 3.587 ± 0.609
3.83GluThr: 3.83 ± 0.531
4.134GluVal: 4.134 ± 0.491
1.216GluTrp: 1.216 ± 0.235
1.763GluTyr: 1.763 ± 0.373
0.0GluXaa: 0.0 ± 0.0
Phe
3.283PheAla: 3.283 ± 0.414
0.243PheCys: 0.243 ± 0.116
2.554PheAsp: 2.554 ± 0.491
2.371PheGlu: 2.371 ± 0.359
0.73PhePhe: 0.73 ± 0.195
3.587PheGly: 3.587 ± 0.438
0.79PheHis: 0.79 ± 0.229
1.52PheIle: 1.52 ± 0.262
1.642PheLys: 1.642 ± 0.332
2.493PheLeu: 2.493 ± 0.491
0.79PheMet: 0.79 ± 0.2
2.006PheAsn: 2.006 ± 0.351
1.824PhePro: 1.824 ± 0.348
1.216PheGln: 1.216 ± 0.299
1.885PheArg: 1.885 ± 0.318
1.763PheSer: 1.763 ± 0.255
2.006PheThr: 2.006 ± 0.29
1.946PheVal: 1.946 ± 0.322
0.365PheTrp: 0.365 ± 0.138
0.79PheTyr: 0.79 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
5.837GlyAla: 5.837 ± 0.493
0.851GlyCys: 0.851 ± 0.211
5.35GlyAsp: 5.35 ± 0.603
4.925GlyGlu: 4.925 ± 0.577
2.675GlyPhe: 2.675 ± 0.393
7.964GlyGly: 7.964 ± 1.254
1.702GlyHis: 1.702 ± 0.331
3.405GlyIle: 3.405 ± 0.708
4.377GlyLys: 4.377 ± 0.495
7.174GlyLeu: 7.174 ± 0.662
2.067GlyMet: 2.067 ± 0.457
3.465GlyAsn: 3.465 ± 0.579
3.405GlyPro: 3.405 ± 0.497
2.675GlyGln: 2.675 ± 0.437
4.377GlyArg: 4.377 ± 0.541
3.83GlySer: 3.83 ± 0.606
5.654GlyThr: 5.654 ± 0.729
6.809GlyVal: 6.809 ± 0.577
1.581GlyTrp: 1.581 ± 0.261
2.797GlyTyr: 2.797 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
1.52HisAla: 1.52 ± 0.334
0.243HisCys: 0.243 ± 0.102
1.763HisAsp: 1.763 ± 0.359
1.885HisGlu: 1.885 ± 0.289
0.547HisPhe: 0.547 ± 0.222
1.763HisGly: 1.763 ± 0.443
0.304HisHis: 0.304 ± 0.145
1.155HisIle: 1.155 ± 0.269
0.973HisLys: 0.973 ± 0.283
1.338HisLeu: 1.338 ± 0.275
0.365HisMet: 0.365 ± 0.138
0.73HisAsn: 0.73 ± 0.205
1.216HisPro: 1.216 ± 0.269
1.034HisGln: 1.034 ± 0.255
1.398HisArg: 1.398 ± 0.308
0.912HisSer: 0.912 ± 0.252
0.79HisThr: 0.79 ± 0.211
1.155HisVal: 1.155 ± 0.288
0.486HisTrp: 0.486 ± 0.184
0.486HisTyr: 0.486 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
5.289IleAla: 5.289 ± 0.579
0.426IleCys: 0.426 ± 0.131
3.648IleAsp: 3.648 ± 0.425
4.925IleGlu: 4.925 ± 0.562
1.338IlePhe: 1.338 ± 0.256
3.648IleGly: 3.648 ± 0.486
1.277IleHis: 1.277 ± 0.231
2.371IleIle: 2.371 ± 0.414
2.554IleLys: 2.554 ± 0.375
4.195IleLeu: 4.195 ± 0.562
0.79IleMet: 0.79 ± 0.188
2.432IleAsn: 2.432 ± 0.441
3.222IlePro: 3.222 ± 0.55
1.398IleGln: 1.398 ± 0.306
3.648IleArg: 3.648 ± 0.555
2.25IleSer: 2.25 ± 0.348
2.554IleThr: 2.554 ± 0.347
2.918IleVal: 2.918 ± 0.509
0.73IleTrp: 0.73 ± 0.183
1.034IleTyr: 1.034 ± 0.224
0.0IleXaa: 0.0 ± 0.0
Lys
5.168LysAla: 5.168 ± 0.693
0.304LysCys: 0.304 ± 0.136
2.371LysAsp: 2.371 ± 0.371
2.797LysGlu: 2.797 ± 0.388
1.702LysPhe: 1.702 ± 0.32
2.979LysGly: 2.979 ± 0.541
0.73LysHis: 0.73 ± 0.248
3.101LysIle: 3.101 ± 0.574
3.587LysLys: 3.587 ± 0.561
4.621LysLeu: 4.621 ± 0.502
1.094LysMet: 1.094 ± 0.24
1.702LysAsn: 1.702 ± 0.349
2.918LysPro: 2.918 ± 0.468
1.885LysGln: 1.885 ± 0.321
3.222LysArg: 3.222 ± 0.479
2.25LysSer: 2.25 ± 0.352
2.857LysThr: 2.857 ± 0.463
3.709LysVal: 3.709 ± 0.505
0.79LysTrp: 0.79 ± 0.237
1.094LysTyr: 1.094 ± 0.237
0.0LysXaa: 0.0 ± 0.0
Leu
8.937LeuAla: 8.937 ± 0.68
0.608LeuCys: 0.608 ± 0.19
4.56LeuAsp: 4.56 ± 0.492
5.654LeuGlu: 5.654 ± 0.573
2.432LeuPhe: 2.432 ± 0.349
5.776LeuGly: 5.776 ± 0.596
1.885LeuHis: 1.885 ± 0.363
4.317LeuIle: 4.317 ± 0.533
3.83LeuLys: 3.83 ± 0.634
4.864LeuLeu: 4.864 ± 0.61
2.493LeuMet: 2.493 ± 0.433
2.675LeuAsn: 2.675 ± 0.334
4.499LeuPro: 4.499 ± 0.502
2.675LeuGln: 2.675 ± 0.402
6.08LeuArg: 6.08 ± 0.78
6.08LeuSer: 6.08 ± 0.588
5.593LeuThr: 5.593 ± 0.724
4.621LeuVal: 4.621 ± 0.58
1.581LeuTrp: 1.581 ± 0.335
2.189LeuTyr: 2.189 ± 0.434
0.0LeuXaa: 0.0 ± 0.0
Met
3.526MetAla: 3.526 ± 0.452
0.061MetCys: 0.061 ± 0.073
1.034MetAsp: 1.034 ± 0.249
1.459MetGlu: 1.459 ± 0.217
0.547MetPhe: 0.547 ± 0.171
2.31MetGly: 2.31 ± 0.405
0.547MetHis: 0.547 ± 0.174
1.824MetIle: 1.824 ± 0.303
1.459MetLys: 1.459 ± 0.342
1.824MetLeu: 1.824 ± 0.32
0.426MetMet: 0.426 ± 0.147
0.912MetAsn: 0.912 ± 0.233
1.277MetPro: 1.277 ± 0.295
0.73MetGln: 0.73 ± 0.218
1.52MetArg: 1.52 ± 0.295
2.25MetSer: 2.25 ± 0.31
2.797MetThr: 2.797 ± 0.354
1.763MetVal: 1.763 ± 0.371
0.182MetTrp: 0.182 ± 0.111
1.034MetTyr: 1.034 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
2.918AsnAla: 2.918 ± 0.437
0.426AsnCys: 0.426 ± 0.14
1.885AsnAsp: 1.885 ± 0.33
1.946AsnGlu: 1.946 ± 0.378
0.973AsnPhe: 0.973 ± 0.23
3.709AsnGly: 3.709 ± 0.421
0.669AsnHis: 0.669 ± 0.195
1.155AsnIle: 1.155 ± 0.23
1.338AsnLys: 1.338 ± 0.247
2.979AsnLeu: 2.979 ± 0.531
0.973AsnMet: 0.973 ± 0.277
0.73AsnAsn: 0.73 ± 0.208
2.736AsnPro: 2.736 ± 0.375
0.79AsnGln: 0.79 ± 0.203
2.25AsnArg: 2.25 ± 0.364
1.277AsnSer: 1.277 ± 0.266
1.702AsnThr: 1.702 ± 0.3
3.04AsnVal: 3.04 ± 0.462
1.034AsnTrp: 1.034 ± 0.25
1.034AsnTyr: 1.034 ± 0.225
0.0AsnXaa: 0.0 ± 0.0
Pro
4.742ProAla: 4.742 ± 0.696
0.304ProCys: 0.304 ± 0.131
4.134ProAsp: 4.134 ± 0.55
4.438ProGlu: 4.438 ± 0.596
2.067ProPhe: 2.067 ± 0.356
4.013ProGly: 4.013 ± 0.473
1.277ProHis: 1.277 ± 0.242
2.25ProIle: 2.25 ± 0.399
2.31ProLys: 2.31 ± 0.392
3.101ProLeu: 3.101 ± 0.357
1.459ProMet: 1.459 ± 0.276
1.702ProAsn: 1.702 ± 0.278
2.189ProPro: 2.189 ± 0.457
1.155ProGln: 1.155 ± 0.277
2.979ProArg: 2.979 ± 0.467
3.648ProSer: 3.648 ± 0.506
3.344ProThr: 3.344 ± 0.613
4.134ProVal: 4.134 ± 0.459
1.277ProTrp: 1.277 ± 0.347
1.398ProTyr: 1.398 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
3.344GlnAla: 3.344 ± 0.358
0.243GlnCys: 0.243 ± 0.119
1.398GlnAsp: 1.398 ± 0.277
1.885GlnGlu: 1.885 ± 0.383
1.277GlnPhe: 1.277 ± 0.279
2.979GlnGly: 2.979 ± 0.525
0.547GlnHis: 0.547 ± 0.164
1.946GlnIle: 1.946 ± 0.307
1.885GlnLys: 1.885 ± 0.34
2.675GlnLeu: 2.675 ± 0.477
0.912GlnMet: 0.912 ± 0.212
1.155GlnAsn: 1.155 ± 0.257
1.398GlnPro: 1.398 ± 0.385
1.642GlnGln: 1.642 ± 0.432
2.128GlnArg: 2.128 ± 0.43
1.763GlnSer: 1.763 ± 0.324
2.128GlnThr: 2.128 ± 0.294
2.128GlnVal: 2.128 ± 0.278
0.669GlnTrp: 0.669 ± 0.165
0.851GlnTyr: 0.851 ± 0.172
0.0GlnXaa: 0.0 ± 0.0
Arg
5.533ArgAla: 5.533 ± 0.655
0.912ArgCys: 0.912 ± 0.252
4.134ArgAsp: 4.134 ± 0.602
5.776ArgGlu: 5.776 ± 0.711
1.946ArgPhe: 1.946 ± 0.4
4.256ArgGly: 4.256 ± 0.612
1.52ArgHis: 1.52 ± 0.313
3.952ArgIle: 3.952 ± 0.432
3.283ArgLys: 3.283 ± 0.561
4.864ArgLeu: 4.864 ± 0.519
2.31ArgMet: 2.31 ± 0.37
1.946ArgAsn: 1.946 ± 0.396
2.675ArgPro: 2.675 ± 0.395
1.824ArgGln: 1.824 ± 0.338
5.411ArgArg: 5.411 ± 0.662
2.554ArgSer: 2.554 ± 0.435
3.344ArgThr: 3.344 ± 0.484
4.681ArgVal: 4.681 ± 0.634
1.216ArgTrp: 1.216 ± 0.283
2.128ArgTyr: 2.128 ± 0.404
0.0ArgXaa: 0.0 ± 0.0
Ser
5.046SerAla: 5.046 ± 0.68
0.365SerCys: 0.365 ± 0.143
3.648SerAsp: 3.648 ± 0.574
3.344SerGlu: 3.344 ± 0.455
2.371SerPhe: 2.371 ± 0.413
5.107SerGly: 5.107 ± 0.742
0.851SerHis: 0.851 ± 0.225
2.736SerIle: 2.736 ± 0.392
2.554SerLys: 2.554 ± 0.485
4.742SerLeu: 4.742 ± 0.664
1.52SerMet: 1.52 ± 0.31
1.155SerAsn: 1.155 ± 0.254
2.918SerPro: 2.918 ± 0.428
1.824SerGln: 1.824 ± 0.39
3.222SerArg: 3.222 ± 0.491
3.161SerSer: 3.161 ± 0.439
3.526SerThr: 3.526 ± 0.553
3.648SerVal: 3.648 ± 0.453
1.155SerTrp: 1.155 ± 0.351
1.52SerTyr: 1.52 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
5.715ThrAla: 5.715 ± 0.484
0.608ThrCys: 0.608 ± 0.182
3.83ThrAsp: 3.83 ± 0.537
3.161ThrGlu: 3.161 ± 0.36
1.702ThrPhe: 1.702 ± 0.318
5.715ThrGly: 5.715 ± 0.86
1.034ThrHis: 1.034 ± 0.268
2.797ThrIle: 2.797 ± 0.442
3.04ThrLys: 3.04 ± 0.547
4.317ThrLeu: 4.317 ± 0.517
1.642ThrMet: 1.642 ± 0.314
1.824ThrAsn: 1.824 ± 0.465
4.013ThrPro: 4.013 ± 0.466
2.371ThrGln: 2.371 ± 0.381
3.04ThrArg: 3.04 ± 0.358
3.101ThrSer: 3.101 ± 0.551
4.134ThrThr: 4.134 ± 0.583
4.317ThrVal: 4.317 ± 0.52
1.398ThrTrp: 1.398 ± 0.257
2.128ThrTyr: 2.128 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
6.323ValAla: 6.323 ± 0.872
0.73ValCys: 0.73 ± 0.2
5.35ValAsp: 5.35 ± 0.546
4.925ValGlu: 4.925 ± 0.583
2.918ValPhe: 2.918 ± 0.429
4.925ValGly: 4.925 ± 0.566
1.277ValHis: 1.277 ± 0.232
3.587ValIle: 3.587 ± 0.496
3.648ValLys: 3.648 ± 0.466
5.593ValLeu: 5.593 ± 0.599
1.52ValMet: 1.52 ± 0.312
2.675ValAsn: 2.675 ± 0.506
3.161ValPro: 3.161 ± 0.418
2.067ValGln: 2.067 ± 0.353
4.925ValArg: 4.925 ± 0.611
3.891ValSer: 3.891 ± 0.51
4.195ValThr: 4.195 ± 0.447
5.411ValVal: 5.411 ± 0.482
1.398ValTrp: 1.398 ± 0.274
1.946ValTyr: 1.946 ± 0.361
0.0ValXaa: 0.0 ± 0.0
Trp
1.702TrpAla: 1.702 ± 0.346
0.365TrpCys: 0.365 ± 0.175
1.094TrpAsp: 1.094 ± 0.294
0.79TrpGlu: 0.79 ± 0.203
0.669TrpPhe: 0.669 ± 0.196
1.398TrpGly: 1.398 ± 0.288
0.365TrpHis: 0.365 ± 0.149
1.094TrpIle: 1.094 ± 0.265
0.486TrpLys: 0.486 ± 0.158
1.338TrpLeu: 1.338 ± 0.271
0.608TrpMet: 0.608 ± 0.188
0.851TrpAsn: 0.851 ± 0.21
0.973TrpPro: 0.973 ± 0.279
1.216TrpGln: 1.216 ± 0.265
1.338TrpArg: 1.338 ± 0.24
1.338TrpSer: 1.338 ± 0.384
1.398TrpThr: 1.398 ± 0.311
1.581TrpVal: 1.581 ± 0.27
0.486TrpTrp: 0.486 ± 0.156
0.669TrpTyr: 0.669 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.614TyrAla: 2.614 ± 0.425
0.182TyrCys: 0.182 ± 0.11
2.067TyrAsp: 2.067 ± 0.31
1.946TyrGlu: 1.946 ± 0.354
0.973TyrPhe: 0.973 ± 0.259
1.946TyrGly: 1.946 ± 0.404
0.547TyrHis: 0.547 ± 0.172
1.702TyrIle: 1.702 ± 0.254
0.79TyrLys: 0.79 ± 0.212
2.979TyrLeu: 2.979 ± 0.409
0.426TyrMet: 0.426 ± 0.205
0.669TyrAsn: 0.669 ± 0.184
1.824TyrPro: 1.824 ± 0.308
1.094TyrGln: 1.094 ± 0.235
2.493TyrArg: 2.493 ± 0.366
1.824TyrSer: 1.824 ± 0.281
1.702TyrThr: 1.702 ± 0.318
2.006TyrVal: 2.006 ± 0.38
0.365TyrTrp: 0.365 ± 0.136
0.608TyrTyr: 0.608 ± 0.18
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 98 proteins (16449 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski