Entoleuca phenui-like virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Entovirus; Entoleuca entovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6Q3I0|A0A4D6Q3I0_9VIRU Movement protein OS=Entoleuca phenui-like virus 1 OX=2086640 PE=4 SV=1
MM1 pKa = 7.38LEE3 pKa = 4.19SVKK6 pKa = 10.04MLRR9 pKa = 11.84KK10 pKa = 9.36FFGRR14 pKa = 11.84RR15 pKa = 11.84GSDD18 pKa = 3.14NKK20 pKa = 10.61PSGARR25 pKa = 11.84PGGSIRR31 pKa = 11.84GSITGSRR38 pKa = 11.84KK39 pKa = 9.25MKK41 pKa = 10.61DD42 pKa = 2.87KK43 pKa = 9.98EE44 pKa = 4.53TEE46 pKa = 3.72DD47 pKa = 3.51RR48 pKa = 11.84EE49 pKa = 4.32ARR51 pKa = 11.84RR52 pKa = 11.84GAVLRR57 pKa = 11.84NMDD60 pKa = 3.74EE61 pKa = 4.28ALFASGVPKK70 pKa = 10.58NHH72 pKa = 5.84VSMADD77 pKa = 3.13KK78 pKa = 10.82LDD80 pKa = 3.51SHH82 pKa = 6.94KK83 pKa = 11.14AGSTFKK89 pKa = 10.61YY90 pKa = 10.41AVIGTQPSEE99 pKa = 3.9EE100 pKa = 3.94RR101 pKa = 11.84TGIMNKK107 pKa = 9.39LRR109 pKa = 11.84NYY111 pKa = 8.8KK112 pKa = 10.41APSKK116 pKa = 10.21LQNFSLSDD124 pKa = 3.27IPFIRR129 pKa = 11.84LMDD132 pKa = 4.33DD133 pKa = 3.19EE134 pKa = 5.81VEE136 pKa = 4.5FSMKK140 pKa = 10.03EE141 pKa = 3.72ALRR144 pKa = 11.84SSNAQKK150 pKa = 10.86HH151 pKa = 5.15EE152 pKa = 4.44DD153 pKa = 3.56YY154 pKa = 11.33CLIDD158 pKa = 3.23SVLIHH163 pKa = 6.25FVPLDD168 pKa = 3.31SFLNDD173 pKa = 3.76KK174 pKa = 11.19SPITIQLNDD183 pKa = 3.42FRR185 pKa = 11.84RR186 pKa = 11.84VNNTVARR193 pKa = 11.84LATVDD198 pKa = 3.12NTMSYY203 pKa = 11.16NILFCLDD210 pKa = 3.48YY211 pKa = 10.95CVEE214 pKa = 4.12SRR216 pKa = 11.84DD217 pKa = 3.97LEE219 pKa = 4.3RR220 pKa = 11.84MTLSFACPQKK230 pKa = 10.81DD231 pKa = 3.85FQPGVAWGAVKK242 pKa = 10.45VIAQVKK248 pKa = 9.25FMSFPVRR255 pKa = 11.84MPVIEE260 pKa = 4.23TMAVAIFADD269 pKa = 3.94TDD271 pKa = 3.92LLEE274 pKa = 4.48YY275 pKa = 10.3EE276 pKa = 3.87WDD278 pKa = 3.53PRR280 pKa = 11.84EE281 pKa = 3.92FDD283 pKa = 4.41AVLGPQALQGVRR295 pKa = 11.84QANKK299 pKa = 10.06RR300 pKa = 11.84GEE302 pKa = 4.18LEE304 pKa = 3.88NLTIAKK310 pKa = 9.1NDD312 pKa = 3.34RR313 pKa = 11.84MEE315 pKa = 4.14VGMARR320 pKa = 11.84TLAMGSLEE328 pKa = 3.99EE329 pKa = 4.46EE330 pKa = 4.51EE331 pKa = 4.46PGEE334 pKa = 4.98AIQALKK340 pKa = 10.62NAALLRR346 pKa = 11.84QRR348 pKa = 11.84QANSTGKK355 pKa = 10.59GKK357 pKa = 10.99GILKK361 pKa = 9.87HH362 pKa = 7.03DD363 pKa = 4.36SFDD366 pKa = 3.83SPTEE370 pKa = 4.0EE371 pKa = 5.53KK372 pKa = 10.76EE373 pKa = 4.24LDD375 pKa = 3.88PDD377 pKa = 4.21DD378 pKa = 4.77SASQVLSGLSEE389 pKa = 4.82EE390 pKa = 4.6IPGPRR395 pKa = 11.84NVTGSRR401 pKa = 11.84TVNFTT406 pKa = 3.2

Molecular weight: 45.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6Q3I0|A0A4D6Q3I0_9VIRU Movement protein OS=Entoleuca phenui-like virus 1 OX=2086640 PE=4 SV=1
MM1 pKa = 7.39SSSSRR6 pKa = 11.84KK7 pKa = 5.44VTGVGGQGQGSSGGPAQGGSQAPSISGMVDD37 pKa = 2.69KK38 pKa = 10.96LGNKK42 pKa = 8.7IWLTNTSTSEE52 pKa = 4.03DD53 pKa = 3.56LTPAKK58 pKa = 10.12ISEE61 pKa = 4.24LVQNVSNDD69 pKa = 4.34DD70 pKa = 3.16ISKK73 pKa = 9.47TITAVEE79 pKa = 4.33SSTTTARR86 pKa = 11.84DD87 pKa = 3.24ALEE90 pKa = 3.88FSIFDD95 pKa = 3.72FQGFDD100 pKa = 3.44PLMVWRR106 pKa = 11.84VLRR109 pKa = 11.84ACQSYY114 pKa = 10.95YY115 pKa = 10.67KK116 pKa = 10.87DD117 pKa = 3.2SDD119 pKa = 3.76EE120 pKa = 5.03NLLSDD125 pKa = 4.02IRR127 pKa = 11.84FSVAAVLYY135 pKa = 9.23MGNLQTKK142 pKa = 9.25ALTRR146 pKa = 11.84RR147 pKa = 11.84AQEE150 pKa = 3.52GRR152 pKa = 11.84TKK154 pKa = 10.18IEE156 pKa = 4.03YY157 pKa = 9.74LVKK160 pKa = 10.3KK161 pKa = 9.95YY162 pKa = 10.8NIRR165 pKa = 11.84TGSSGAGLEE174 pKa = 4.33AEE176 pKa = 4.36TLTFPRR182 pKa = 11.84IAAAAPVFAVKK193 pKa = 10.22LANQLPPSAVSIAFMGRR210 pKa = 11.84EE211 pKa = 4.22VPSPMRR217 pKa = 11.84LMPFASLCSPQMDD230 pKa = 3.73TEE232 pKa = 4.13LRR234 pKa = 11.84KK235 pKa = 9.5FLLQACNAHH244 pKa = 6.33ASDD247 pKa = 3.72MGLAYY252 pKa = 10.05EE253 pKa = 5.14RR254 pKa = 11.84GRR256 pKa = 11.84CKK258 pKa = 10.11KK259 pKa = 10.17AKK261 pKa = 9.93KK262 pKa = 10.17EE263 pKa = 3.9FVANPKK269 pKa = 9.98EE270 pKa = 3.95MAEE273 pKa = 4.22DD274 pKa = 3.03QWAFIEE280 pKa = 4.46VASDD284 pKa = 3.64SPVPEE289 pKa = 4.15EE290 pKa = 3.96SMKK293 pKa = 10.75RR294 pKa = 11.84STLLEE299 pKa = 4.55LNLSQYY305 pKa = 10.4YY306 pKa = 9.35KK307 pKa = 10.57QLAEE311 pKa = 3.93VVRR314 pKa = 11.84NYY316 pKa = 10.65RR317 pKa = 11.84SIMEE321 pKa = 4.28GSEE324 pKa = 3.85KK325 pKa = 10.23STVKK329 pKa = 10.62VMSQSEE335 pKa = 4.42FDD337 pKa = 3.5TKK339 pKa = 10.87LSEE342 pKa = 4.97FISSRR347 pKa = 11.84AMDD350 pKa = 3.66TQQ352 pKa = 3.31

Molecular weight: 38.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3111

352

2353

1037.0

118.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.625 ± 1.479

1.382 ± 0.16

6.107 ± 0.426

7.49 ± 0.208

4.114 ± 0.158

5.239 ± 0.469

2.089 ± 0.664

5.947 ± 0.851

7.425 ± 0.536

9.129 ± 0.345

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.6 ± 0.175

4.629 ± 0.331

3.729 ± 0.37

3.632 ± 0.331

5.561 ± 0.456

8.454 ± 0.978

4.982 ± 0.265

6.011 ± 0.095

1.446 ± 0.384

3.407 ± 0.706
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski