Paramecium tetraurelia

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Oligohymenophorea; Peniculida; Parameciidae; Paramecium

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0DUY7|A0DUY7_PARTE Uncharacterized protein OS=Paramecium tetraurelia OX=5888 GN=GSPATT00020516001 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.15IVLITLLLGLTFSQNQALNEE22 pKa = 3.91ISEE25 pKa = 4.39NPIPTLVYY33 pKa = 7.39EE34 pKa = 4.66TKK36 pKa = 10.66NEE38 pKa = 3.88EE39 pKa = 4.03SQQIQQHH46 pKa = 4.8QQLQGYY52 pKa = 9.24IDD54 pKa = 3.79IDD56 pKa = 3.61GHH58 pKa = 5.57FVSNQADD65 pKa = 3.46NTQNTKK71 pKa = 10.34SEE73 pKa = 4.26PAVQTNQEE81 pKa = 4.1EE82 pKa = 4.7TQNSQVTISDD92 pKa = 3.89DD93 pKa = 4.04SNNLQATTQEE103 pKa = 4.59GAQQLVSTDD112 pKa = 3.68AEE114 pKa = 4.18IDD116 pKa = 3.5PMFDD120 pKa = 3.71MEE122 pKa = 5.03NSMPYY127 pKa = 9.87EE128 pKa = 4.32FPEE131 pKa = 4.18DD132 pKa = 3.5TQVQEE137 pKa = 4.13EE138 pKa = 4.77SKK140 pKa = 11.29DD141 pKa = 3.59QDD143 pKa = 3.24GDD145 pKa = 3.45NHH147 pKa = 6.41YY148 pKa = 11.03LSASLEE154 pKa = 4.04LPIRR158 pKa = 11.84NEE160 pKa = 3.74NQVYY164 pKa = 10.55DD165 pKa = 3.85SLGNQIDD172 pKa = 4.28SYY174 pKa = 11.74DD175 pKa = 3.37GMRR178 pKa = 11.84TYY180 pKa = 11.1LALDD184 pKa = 3.24IAEE187 pKa = 4.29QDD189 pKa = 3.75DD190 pKa = 3.93NQANISVQEE199 pKa = 4.04KK200 pKa = 10.3QEE202 pKa = 4.04DD203 pKa = 4.46CIVIYY208 pKa = 9.41SKK210 pKa = 11.29CDD212 pKa = 3.52FQGEE216 pKa = 4.46SLPICEE222 pKa = 4.33SLKK225 pKa = 10.65EE226 pKa = 4.13VEE228 pKa = 4.15QNQAKK233 pKa = 10.16ILGISNMISSPSIYY247 pKa = 10.57LL248 pKa = 3.49

Molecular weight: 27.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0C0Z0|A0C0Z0_PARTE Uncharacterized protein OS=Paramecium tetraurelia OX=5888 GN=GSPATT00033933001 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 9.62PKK5 pKa = 9.16VTKK8 pKa = 10.17KK9 pKa = 9.98AAKK12 pKa = 9.32IVKK15 pKa = 9.89KK16 pKa = 10.6KK17 pKa = 9.59IAKK20 pKa = 9.81KK21 pKa = 8.94STGPRR26 pKa = 11.84PTRR29 pKa = 11.84TIQATVAQAQATTQPSAGQRR49 pKa = 11.84KK50 pKa = 8.7LPRR53 pKa = 11.84QNRR56 pKa = 11.84RR57 pKa = 11.84NKK59 pKa = 7.97QAKK62 pKa = 9.24GKK64 pKa = 9.58SNKK67 pKa = 9.15KK68 pKa = 8.89AQKK71 pKa = 10.03KK72 pKa = 9.21

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39461

0

39461

18009409

33

7119

456.4

53.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.478 ± 0.009

1.823 ± 0.02

5.001 ± 0.008

6.68 ± 0.016

4.95 ± 0.011

3.559 ± 0.012

1.764 ± 0.005

8.432 ± 0.013

8.828 ± 0.016

9.741 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.102 ± 0.005

6.908 ± 0.011

2.915 ± 0.008

9.112 ± 0.017

3.452 ± 0.008

6.906 ± 0.011

4.539 ± 0.01

4.455 ± 0.008

0.763 ± 0.004

4.252 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski