Corynebacterium sp. HMSC08D02
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2124 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1F1UXR2|A0A1F1UXR2_9CORY tRNA glutamyl-Q synthetase OS=Corynebacterium sp. HMSC08D02 OX=1581138 GN=HMPREF3170_01380 PE=3 SV=1
MM1 pKa = 7.35 MSLNPDD7 pKa = 3.84 FYY9 pKa = 11.34 YY10 pKa = 10.73 SEE12 pKa = 3.87 DD13 pKa = 3.94 HH14 pKa = 5.92 EE15 pKa = 5.03 WINARR20 pKa = 11.84 PEE22 pKa = 3.93 DD23 pKa = 3.65 AAGTKK28 pKa = 10.06 VRR30 pKa = 11.84 VGITNVAADD39 pKa = 4.0 RR40 pKa = 11.84 LGEE43 pKa = 4.25 VVFAEE48 pKa = 4.78 LPQVGDD54 pKa = 3.28 QVTAGEE60 pKa = 4.44 TCGEE64 pKa = 4.21 VEE66 pKa = 4.36 STKK69 pKa = 10.75 SVSDD73 pKa = 3.74 LYY75 pKa = 11.51 APVTGTVTAVNEE87 pKa = 5.03 DD88 pKa = 2.77 IDD90 pKa = 4.08 GAYY93 pKa = 9.85 EE94 pKa = 5.06 AINEE98 pKa = 4.25 DD99 pKa = 3.77 PFGAGWLFEE108 pKa = 4.38 VEE110 pKa = 4.24 VEE112 pKa = 4.25 EE113 pKa = 4.94 VGPLMTADD121 pKa = 4.24 EE122 pKa = 4.3 YY123 pKa = 11.66 AAANGVEE130 pKa = 4.09 NN131 pKa = 4.31
Molecular weight: 14.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 3.706
IPC_protein 3.643
Toseland 3.452
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.478
Grimsley 3.376
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.872
Thurlkill 3.503
EMBOSS 3.528
Sillero 3.757
Patrickios 1.799
IPC_peptide 3.592
IPC2_peptide 3.732
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|A0A1F1UVE9|A0A1F1UVE9_9CORY Thioredoxin reductase OS=Corynebacterium sp. HMSC08D02 OX=1581138 GN=HMPREF3170_05425 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 KK16 pKa = 8.14 HH17 pKa = 4.83 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVSARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.63 KK38 pKa = 10.53 GRR40 pKa = 11.84 ASLTAA45 pKa = 4.1
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2124
0
2124
703705
40
2502
331.3
35.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.627 ± 0.082
0.701 ± 0.015
6.039 ± 0.049
6.527 ± 0.054
3.342 ± 0.033
8.16 ± 0.053
2.14 ± 0.027
4.899 ± 0.038
3.459 ± 0.052
9.349 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.026
2.91 ± 0.036
5.102 ± 0.046
3.58 ± 0.032
6.054 ± 0.06
5.558 ± 0.039
6.221 ± 0.054
8.433 ± 0.054
1.363 ± 0.021
2.282 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here