Listeria phage LP-030-2
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R4ICE2|R4ICE2_9CAUD Uncharacterized protein OS=Listeria phage LP-030-2 OX=1173743 GN=LP030nr2_054 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.14 KK3 pKa = 10.4 CNLCGEE9 pKa = 4.36 VFKK12 pKa = 11.29 EE13 pKa = 3.74 FDD15 pKa = 3.39 EE16 pKa = 4.55 VVLVNEE22 pKa = 3.74 RR23 pKa = 11.84 DD24 pKa = 3.94 YY25 pKa = 11.62 FHH27 pKa = 7.09 EE28 pKa = 4.66 ACVALSPIKK37 pKa = 10.6 YY38 pKa = 10.12 AVFEE42 pKa = 4.18 NSGDD46 pKa = 3.55 ANYY49 pKa = 11.12 DD50 pKa = 3.64 DD51 pKa = 6.62 FIGTCEE57 pKa = 3.87 PSEE60 pKa = 4.16 IEE62 pKa = 4.25 CALLLFDD69 pKa = 4.76 EE70 pKa = 4.91 GDD72 pKa = 3.75 YY73 pKa = 11.37 LKK75 pKa = 11.0 EE76 pKa = 4.81 GEE78 pKa = 4.58 DD79 pKa = 3.77 KK80 pKa = 11.01 EE81 pKa = 4.38
Molecular weight: 9.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 4.075
IPC_protein 3.986
Toseland 3.808
ProMoST 4.075
Dawson 3.935
Bjellqvist 4.151
Wikipedia 3.821
Rodwell 3.821
Grimsley 3.719
Solomon 3.935
Lehninger 3.884
Nozaki 4.062
DTASelect 4.19
Thurlkill 3.834
EMBOSS 3.846
Sillero 4.101
Patrickios 0.947
IPC_peptide 3.935
IPC2_peptide 4.075
IPC2.peptide.svr19 4.023
Protein with the highest isoelectric point:
>tr|R4ICD6|R4ICD6_9CAUD YopX-like domain containing protein OS=Listeria phage LP-030-2 OX=1173743 GN=LP030nr2_044 PE=4 SV=1
MM1 pKa = 7.43 GKK3 pKa = 10.33 YY4 pKa = 8.07 YY5 pKa = 10.08 WHH7 pKa = 6.92 VSRR10 pKa = 11.84 LGGKK14 pKa = 6.75 PTEE17 pKa = 3.81 IRR19 pKa = 11.84 HH20 pKa = 5.37 YY21 pKa = 10.6 KK22 pKa = 10.26 HH23 pKa = 5.13 ITKK26 pKa = 9.48 MYY28 pKa = 10.53 KK29 pKa = 10.29 FILRR33 pKa = 11.84 NPAMFKK39 pKa = 10.89 DD40 pKa = 3.79 KK41 pKa = 10.4 TLTIYY46 pKa = 11.07 DD47 pKa = 3.48 HH48 pKa = 7.07 AKK50 pKa = 9.8 PVTNMTFNEE59 pKa = 3.72 IKK61 pKa = 10.7 YY62 pKa = 9.87 RR63 pKa = 11.84 ASLNLCEE70 pKa = 4.24 TVEE73 pKa = 4.09 RR74 pKa = 11.84 KK75 pKa = 9.52 YY76 pKa = 11.5 VLGLKK81 pKa = 9.69 QRR83 pKa = 11.84 LTEE86 pKa = 4.07 EE87 pKa = 3.78 QKK89 pKa = 10.49 EE90 pKa = 4.1 ARR92 pKa = 11.84 KK93 pKa = 9.79
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.132
IPC2_protein 9.443
IPC_protein 9.414
Toseland 10.058
ProMoST 9.78
Dawson 10.262
Bjellqvist 9.911
Wikipedia 10.423
Rodwell 10.774
Grimsley 10.335
Solomon 10.277
Lehninger 10.248
Nozaki 10.043
DTASelect 9.911
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.175
Patrickios 10.423
IPC_peptide 10.277
IPC2_peptide 8.492
IPC2.peptide.svr19 8.405
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
11750
37
1026
170.3
19.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.323 ± 0.516
0.996 ± 0.155
6.247 ± 0.173
8.357 ± 0.306
4.426 ± 0.217
5.549 ± 0.322
1.685 ± 0.23
7.106 ± 0.182
8.987 ± 0.305
8.094 ± 0.324
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.791 ± 0.138
6.528 ± 0.266
2.664 ± 0.194
3.498 ± 0.2
3.498 ± 0.242
5.719 ± 0.199
5.677 ± 0.33
6.281 ± 0.245
1.345 ± 0.158
4.23 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here