Wenzhou shrimp virus 10
Average proteome isoelectric point is 8.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIL9|A0A1L3KIL9_9VIRU RNA-dependent RNA polymerase OS=Wenzhou shrimp virus 10 OX=1923649 PE=4 SV=1
MM1 pKa = 7.17 STLDD5 pKa = 3.44 TYY7 pKa = 11.79 ANCIRR12 pKa = 11.84 VWHH15 pKa = 5.92 QINFKK20 pKa = 10.86 GKK22 pKa = 9.91 FSFHH26 pKa = 6.55 LKK28 pKa = 9.78 SQLSHH33 pKa = 5.7 FQRR36 pKa = 11.84 VFSPYY41 pKa = 9.47 PQKK44 pKa = 10.41 QWPKK48 pKa = 9.65 IFKK51 pKa = 10.41 GLLAHH56 pKa = 6.96 KK57 pKa = 9.69 FSVIHH62 pKa = 5.31 EE63 pKa = 4.24 QEE65 pKa = 4.14 LPEE68 pKa = 4.33 VIMDD72 pKa = 4.31 SKK74 pKa = 11.32 HH75 pKa = 6.12 LFYY78 pKa = 10.38 PQYY81 pKa = 10.05 HH82 pKa = 5.96 RR83 pKa = 11.84 RR84 pKa = 11.84 IHH86 pKa = 6.29 SYY88 pKa = 10.68 VVTKK92 pKa = 10.66 YY93 pKa = 10.76 DD94 pKa = 3.21 EE95 pKa = 4.17 EE96 pKa = 4.69 RR97 pKa = 11.84 LVRR100 pKa = 11.84 VFWDD104 pKa = 3.71 LMQTKK109 pKa = 9.9 ALAARR114 pKa = 11.84 VPDD117 pKa = 4.07 SFIKK121 pKa = 10.62 DD122 pKa = 3.33 ALVKK126 pKa = 10.61 HH127 pKa = 6.36 SEE129 pKa = 4.32 TVGQVTSTDD138 pKa = 3.49 PAVLEE143 pKa = 4.42 KK144 pKa = 10.16 FAKK147 pKa = 10.0 FCEE150 pKa = 4.29 PFMKK154 pKa = 9.55 RR155 pKa = 11.84 TGMLLSQQYY164 pKa = 11.11 AGLPKK169 pKa = 10.35 NHH171 pKa = 7.02 ASYY174 pKa = 10.67 EE175 pKa = 4.27 FKK177 pKa = 10.67 RR178 pKa = 11.84 GLGGVGRR185 pKa = 11.84 SYY187 pKa = 11.05 KK188 pKa = 10.07 SSYY191 pKa = 10.21 MNVTQPSQPRR201 pKa = 11.84 LDD203 pKa = 5.22 PITILVSGEE212 pKa = 4.02 PGCGKK217 pKa = 10.34 SLLQNKK223 pKa = 8.9 IASKK227 pKa = 10.27 LAARR231 pKa = 11.84 LDD233 pKa = 3.72 RR234 pKa = 11.84 SLSEE238 pKa = 3.84 TVYY241 pKa = 10.61 VRR243 pKa = 11.84 NSSTRR248 pKa = 11.84 HH249 pKa = 4.31 WDD251 pKa = 3.53 GYY253 pKa = 9.15 KK254 pKa = 9.65 QQPLAMIDD262 pKa = 4.57 DD263 pKa = 4.99 FLQTSHH269 pKa = 6.52 KK270 pKa = 10.53 QSTEE274 pKa = 3.61 PLEE277 pKa = 4.23 AEE279 pKa = 4.27 EE280 pKa = 5.83 FITLNSTCDD289 pKa = 3.37 HH290 pKa = 6.32 VLPMADD296 pKa = 3.52 LRR298 pKa = 11.84 EE299 pKa = 3.97 KK300 pKa = 10.88 GMRR303 pKa = 11.84 FTSPIILYY311 pKa = 10.16 SSNHH315 pKa = 6.32 SYY317 pKa = 10.23 HH318 pKa = 5.9 QATGKK323 pKa = 10.41 LSRR326 pKa = 11.84 QLHH329 pKa = 6.34 SIGAICRR336 pKa = 11.84 RR337 pKa = 11.84 IKK339 pKa = 10.58 FYY341 pKa = 10.56 IEE343 pKa = 3.28 KK344 pKa = 10.45 RR345 pKa = 11.84 AGKK348 pKa = 9.03 WILFKK353 pKa = 10.72 DD354 pKa = 4.18 VPSDD358 pKa = 2.92 IPGRR362 pKa = 11.84 VRR364 pKa = 11.84 TAFDD368 pKa = 3.63 RR369 pKa = 11.84 VCDD372 pKa = 4.2 CGQEE376 pKa = 4.11 TSINGAPSHH385 pKa = 6.03 GRR387 pKa = 11.84 SSVRR391 pKa = 11.84 VLSSNLLHH399 pKa = 6.99 KK400 pKa = 10.26 FAEE403 pKa = 4.22 QVVDD407 pKa = 3.42 ILLGEE412 pKa = 4.08 WEE414 pKa = 4.49 RR415 pKa = 11.84 RR416 pKa = 11.84 CIFYY420 pKa = 10.76 DD421 pKa = 3.37 NNIAEE426 pKa = 5.02 GYY428 pKa = 9.08 SRR430 pKa = 11.84 VPIGPGVSARR440 pKa = 11.84 FEE442 pKa = 4.32 PVSKK446 pKa = 9.53 PHH448 pKa = 6.01 NRR450 pKa = 11.84 VKK452 pKa = 10.52 VVPLTEE458 pKa = 4.09 PLKK461 pKa = 11.01 VRR463 pKa = 11.84 TITVGTAEE471 pKa = 4.33 NFLLKK476 pKa = 10.14 PLQKK480 pKa = 10.96 ALLQSLKK487 pKa = 10.58 GYY489 pKa = 10.52 AQFKK493 pKa = 10.25 PCFDD497 pKa = 4.92 PNYY500 pKa = 9.88 QDD502 pKa = 4.81 QIDD505 pKa = 4.06 SLRR508 pKa = 11.84 LRR510 pKa = 11.84 EE511 pKa = 4.13 GLWLSGDD518 pKa = 3.74 YY519 pKa = 10.64 TSATDD524 pKa = 4.95 GLHH527 pKa = 6.26 QDD529 pKa = 4.25 LFHH532 pKa = 7.22 AGLSSLEE539 pKa = 4.18 SYY541 pKa = 10.47 IPEE544 pKa = 4.22 NLWNLVKK551 pKa = 10.55 RR552 pKa = 11.84 EE553 pKa = 4.18 MEE555 pKa = 3.97 PHH557 pKa = 5.69 LCEE560 pKa = 4.43 YY561 pKa = 8.65 PPEE564 pKa = 4.14 MGIPDD569 pKa = 4.02 CWQTNGQLMGSLLSFPLLCLANAFTLSHH597 pKa = 5.99 TMGKK601 pKa = 9.25 PVGKK605 pKa = 10.27 LPALFHH611 pKa = 7.3 GDD613 pKa = 3.59 DD614 pKa = 4.5 LLAKK618 pKa = 7.31 VTPRR622 pKa = 11.84 EE623 pKa = 3.92 YY624 pKa = 10.39 EE625 pKa = 3.57 AWKK628 pKa = 10.48 AFCPTIGLSLSVGKK642 pKa = 10.48 NYY644 pKa = 9.95 VSPNWGSIDD653 pKa = 3.43 SQVFYY658 pKa = 10.71 QGRR661 pKa = 11.84 CLGTGKK667 pKa = 10.5 FGALSAGHH675 pKa = 5.62 TQSIPTLIRR684 pKa = 11.84 RR685 pKa = 11.84 GVPKK689 pKa = 10.61 GLIVSIFKK697 pKa = 10.74 KK698 pKa = 10.25 EE699 pKa = 3.96 LEE701 pKa = 4.13 KK702 pKa = 10.47 TPRR705 pKa = 11.84 SLDD708 pKa = 3.29 VSHH711 pKa = 7.27 LYY713 pKa = 10.78 GGLGQDD719 pKa = 4.84 GLPQTDD725 pKa = 4.37 LDD727 pKa = 3.83 FQVYY731 pKa = 8.57 HH732 pKa = 6.77 RR733 pKa = 11.84 KK734 pKa = 8.89 IARR737 pKa = 11.84 TFVRR741 pKa = 11.84 RR742 pKa = 11.84 DD743 pKa = 3.21 MRR745 pKa = 11.84 DD746 pKa = 2.31 IGYY749 pKa = 10.1 VYY751 pKa = 10.28 TIDD754 pKa = 3.68 RR755 pKa = 11.84 SYY757 pKa = 11.76 SNSLPAGCVPSEE769 pKa = 4.05 IPEE772 pKa = 4.27 DD773 pKa = 3.91 AEE775 pKa = 4.43 SKK777 pKa = 10.5 RR778 pKa = 11.84 VSDD781 pKa = 3.45 WKK783 pKa = 10.76 GIKK786 pKa = 9.83 HH787 pKa = 6.26 FSSKK791 pKa = 10.83 APATIRR797 pKa = 11.84 VTDD800 pKa = 5.29 PITVFSRR807 pKa = 11.84 KK808 pKa = 8.61 PAPVLEE814 pKa = 3.82 NLVRR818 pKa = 11.84 HH819 pKa = 5.73 SRR821 pKa = 11.84 ADD823 pKa = 3.47 CTVSLDD829 pKa = 4.31 EE830 pKa = 5.43 IDD832 pKa = 3.75 FF833 pKa = 4.33
Molecular weight: 94.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.497
IPC2_protein 8.419
IPC_protein 8.258
Toseland 8.756
ProMoST 8.814
Dawson 9.194
Bjellqvist 9.151
Wikipedia 9.326
Rodwell 9.341
Grimsley 9.136
Solomon 9.238
Lehninger 9.194
Nozaki 9.297
DTASelect 9.019
Thurlkill 9.121
EMBOSS 9.341
Sillero 9.311
Patrickios 4.584
IPC_peptide 9.224
IPC2_peptide 7.907
IPC2.peptide.svr19 7.702
Protein with the highest isoelectric point:
>tr|A0A1L3KIL9|A0A1L3KIL9_9VIRU RNA-dependent RNA polymerase OS=Wenzhou shrimp virus 10 OX=1923649 PE=4 SV=1
MM1 pKa = 7.17 STLDD5 pKa = 3.44 TYY7 pKa = 11.79 ANCIRR12 pKa = 11.84 VWHH15 pKa = 5.92 QINFKK20 pKa = 10.86 GKK22 pKa = 9.91 FSFHH26 pKa = 6.55 LKK28 pKa = 9.78 SQLSHH33 pKa = 5.7 FQRR36 pKa = 11.84 VFSPYY41 pKa = 9.47 PQKK44 pKa = 10.41 QWPKK48 pKa = 9.65 IFKK51 pKa = 10.41 GLLAHH56 pKa = 6.96 KK57 pKa = 9.69 FSVIHH62 pKa = 5.31 EE63 pKa = 4.24 QEE65 pKa = 4.14 LPEE68 pKa = 4.33 VIMDD72 pKa = 4.31 SKK74 pKa = 11.32 HH75 pKa = 6.12 LFYY78 pKa = 10.38 PQYY81 pKa = 10.05 HH82 pKa = 5.96 RR83 pKa = 11.84 RR84 pKa = 11.84 IHH86 pKa = 6.29 SYY88 pKa = 10.68 VVTKK92 pKa = 10.66 YY93 pKa = 10.76 DD94 pKa = 3.21 EE95 pKa = 4.17 EE96 pKa = 4.69 RR97 pKa = 11.84 LVRR100 pKa = 11.84 VFWDD104 pKa = 3.71 LMQTKK109 pKa = 9.9 ALAARR114 pKa = 11.84 VPDD117 pKa = 4.07 SFIKK121 pKa = 10.62 DD122 pKa = 3.33 ALVKK126 pKa = 10.61 HH127 pKa = 6.36 SEE129 pKa = 4.32 TVGQVTSTDD138 pKa = 3.49 PAVLEE143 pKa = 4.42 KK144 pKa = 10.16 FAKK147 pKa = 10.0 FCEE150 pKa = 4.29 PFMKK154 pKa = 9.55 RR155 pKa = 11.84 TGMLLSQQYY164 pKa = 11.11 AGLPKK169 pKa = 10.35 NHH171 pKa = 7.02 ASYY174 pKa = 10.67 EE175 pKa = 4.27 FKK177 pKa = 10.67 RR178 pKa = 11.84 GLGGVGRR185 pKa = 11.84 SYY187 pKa = 11.05 KK188 pKa = 10.07 SSYY191 pKa = 10.21 MNVTQPSQPRR201 pKa = 11.84 LDD203 pKa = 5.22 PITILVSGEE212 pKa = 4.02 PGCGKK217 pKa = 10.34 SLLQNKK223 pKa = 8.9 IASKK227 pKa = 10.27 LAARR231 pKa = 11.84 LDD233 pKa = 3.72 RR234 pKa = 11.84 SLSEE238 pKa = 3.84 TVYY241 pKa = 10.61 VRR243 pKa = 11.84 NSSTRR248 pKa = 11.84 HH249 pKa = 4.31 WDD251 pKa = 3.53 GYY253 pKa = 9.15 KK254 pKa = 9.65 QQPLAMIDD262 pKa = 4.57 DD263 pKa = 4.99 FLQTSHH269 pKa = 6.52 KK270 pKa = 10.53 QSTEE274 pKa = 3.61 PLEE277 pKa = 4.23 AEE279 pKa = 4.27 EE280 pKa = 5.83 FITLNSTCDD289 pKa = 3.37 HH290 pKa = 6.32 VLPMADD296 pKa = 3.52 LRR298 pKa = 11.84 EE299 pKa = 3.97 KK300 pKa = 10.88 GMRR303 pKa = 11.84 FTSPIILYY311 pKa = 10.16 SSNHH315 pKa = 6.32 SYY317 pKa = 10.23 HH318 pKa = 5.9 QATGKK323 pKa = 10.41 LSRR326 pKa = 11.84 QLHH329 pKa = 6.34 SIGAICRR336 pKa = 11.84 RR337 pKa = 11.84 IKK339 pKa = 10.58 FYY341 pKa = 10.56 IEE343 pKa = 3.28 KK344 pKa = 10.45 RR345 pKa = 11.84 AGKK348 pKa = 9.03 WILFKK353 pKa = 10.72 DD354 pKa = 4.18 VPSDD358 pKa = 2.92 IPGRR362 pKa = 11.84 VRR364 pKa = 11.84 TAFDD368 pKa = 3.63 RR369 pKa = 11.84 VCDD372 pKa = 4.2 CGQEE376 pKa = 4.11 TSINGAPSHH385 pKa = 6.03 GRR387 pKa = 11.84 SSVRR391 pKa = 11.84 VLSSNLLHH399 pKa = 6.99 KK400 pKa = 10.26 FAEE403 pKa = 4.22 QVVDD407 pKa = 3.42 ILLGEE412 pKa = 4.08 WEE414 pKa = 4.49 RR415 pKa = 11.84 RR416 pKa = 11.84 CIFYY420 pKa = 10.76 DD421 pKa = 3.37 NNIAEE426 pKa = 5.02 GYY428 pKa = 9.08 SRR430 pKa = 11.84 VPIGPGVSARR440 pKa = 11.84 FEE442 pKa = 4.32 PVSKK446 pKa = 9.53 PHH448 pKa = 6.01 NRR450 pKa = 11.84 VKK452 pKa = 10.52 VVPLTEE458 pKa = 4.09 PLKK461 pKa = 11.01 VRR463 pKa = 11.84 TITVGTAEE471 pKa = 4.33 NFLLKK476 pKa = 10.14 PLQKK480 pKa = 10.96 ALLQSLKK487 pKa = 10.58 GYY489 pKa = 10.52 AQFKK493 pKa = 10.25 PCFDD497 pKa = 4.92 PNYY500 pKa = 9.88 QDD502 pKa = 4.81 QIDD505 pKa = 4.06 SLRR508 pKa = 11.84 LRR510 pKa = 11.84 EE511 pKa = 4.13 GLWLSGDD518 pKa = 3.74 YY519 pKa = 10.64 TSATDD524 pKa = 4.95 GLHH527 pKa = 6.26 QDD529 pKa = 4.25 LFHH532 pKa = 7.22 AGLSSLEE539 pKa = 4.18 SYY541 pKa = 10.47 IPEE544 pKa = 4.22 NLWNLVKK551 pKa = 10.55 RR552 pKa = 11.84 EE553 pKa = 4.18 MEE555 pKa = 3.97 PHH557 pKa = 5.69 LCEE560 pKa = 4.43 YY561 pKa = 8.65 PPEE564 pKa = 4.14 MGIPDD569 pKa = 4.02 CWQTNGQLMGSLLSFPLLCLANAFTLSHH597 pKa = 5.99 TMGKK601 pKa = 9.25 PVGKK605 pKa = 10.27 LPALFHH611 pKa = 7.3 GDD613 pKa = 3.59 DD614 pKa = 4.5 LLAKK618 pKa = 7.31 VTPRR622 pKa = 11.84 EE623 pKa = 3.92 YY624 pKa = 10.39 EE625 pKa = 3.57 AWKK628 pKa = 10.48 AFCPTIGLSLSVGKK642 pKa = 10.48 NYY644 pKa = 9.95 VSPNWGSIDD653 pKa = 3.43 SQVFYY658 pKa = 10.71 QGRR661 pKa = 11.84 CLGTGKK667 pKa = 10.5 FGALSAGHH675 pKa = 5.62 TQSIPTLIRR684 pKa = 11.84 RR685 pKa = 11.84 GVPKK689 pKa = 10.61 GLIVSIFKK697 pKa = 10.74 KK698 pKa = 10.25 EE699 pKa = 3.96 LEE701 pKa = 4.13 KK702 pKa = 10.47 TPRR705 pKa = 11.84 SLDD708 pKa = 3.29 VSHH711 pKa = 7.27 LYY713 pKa = 10.78 GGLGQDD719 pKa = 4.84 GLPQTDD725 pKa = 4.37 LDD727 pKa = 3.83 FQVYY731 pKa = 8.57 HH732 pKa = 6.77 RR733 pKa = 11.84 KK734 pKa = 8.89 IARR737 pKa = 11.84 TFVRR741 pKa = 11.84 RR742 pKa = 11.84 DD743 pKa = 3.21 MRR745 pKa = 11.84 DD746 pKa = 2.31 IGYY749 pKa = 10.1 VYY751 pKa = 10.28 TIDD754 pKa = 3.68 RR755 pKa = 11.84 SYY757 pKa = 11.76 SNSLPAGCVPSEE769 pKa = 4.05 IPEE772 pKa = 4.27 DD773 pKa = 3.91 AEE775 pKa = 4.43 SKK777 pKa = 10.5 RR778 pKa = 11.84 VSDD781 pKa = 3.45 WKK783 pKa = 10.76 GIKK786 pKa = 9.83 HH787 pKa = 6.26 FSSKK791 pKa = 10.83 APATIRR797 pKa = 11.84 VTDD800 pKa = 5.29 PITVFSRR807 pKa = 11.84 KK808 pKa = 8.61 PAPVLEE814 pKa = 3.82 NLVRR818 pKa = 11.84 HH819 pKa = 5.73 SRR821 pKa = 11.84 ADD823 pKa = 3.47 CTVSLDD829 pKa = 4.31 EE830 pKa = 5.43 IDD832 pKa = 3.75 FF833 pKa = 4.33
Molecular weight: 94.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.497
IPC2_protein 8.419
IPC_protein 8.258
Toseland 8.756
ProMoST 8.814
Dawson 9.194
Bjellqvist 9.151
Wikipedia 9.326
Rodwell 9.341
Grimsley 9.136
Solomon 9.238
Lehninger 9.194
Nozaki 9.297
DTASelect 9.019
Thurlkill 9.121
EMBOSS 9.341
Sillero 9.311
Patrickios 4.584
IPC_peptide 9.224
IPC2_peptide 7.907
IPC2.peptide.svr19 7.702
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
833
833
833
833.0
94.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.282 ± 0.0
1.921 ± 0.0
5.162 ± 0.0
4.922 ± 0.0
4.682 ± 0.0
6.483 ± 0.0
3.481 ± 0.0
5.162 ± 0.0
6.483 ± 0.0
10.084 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.681 ± 0.0
2.761 ± 0.0
6.122 ± 0.0
4.322 ± 0.0
6.122 ± 0.0
8.884 ± 0.0
5.042 ± 0.0
6.363 ± 0.0
1.441 ± 0.0
3.601 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here