Moumouvirus australiensis
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 882 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1EM83|A0A2P1EM83_9VIRU Uncharacterized protein OS=Moumouvirus australiensis OX=2109587 GN=mc_619 PE=4 SV=1
MM1 pKa = 7.44 GNSTSTNTQTLEE13 pKa = 3.93 NQPLINSVSEE23 pKa = 4.61 DD24 pKa = 3.67 NVLMNYY30 pKa = 9.64 QSSTEE35 pKa = 4.11 TNEE38 pKa = 3.44 EE39 pKa = 4.08 VIPVKK44 pKa = 10.28 KK45 pKa = 10.23 SSYY48 pKa = 9.33 IHH50 pKa = 6.4 PLINLITVNTTDD62 pKa = 5.22 DD63 pKa = 5.21 DD64 pKa = 5.39 IIDD67 pKa = 4.03 TFNAYY72 pKa = 10.32 CGVQKK77 pKa = 10.99 LSDD80 pKa = 4.03 NLYY83 pKa = 10.25 DD84 pKa = 4.84 IPDD87 pKa = 3.42 QYY89 pKa = 11.83 EE90 pKa = 3.91 VFGPVMQLFCHH101 pKa = 6.25 CACYY105 pKa = 10.31 GRR107 pKa = 11.84 KK108 pKa = 9.22 KK109 pKa = 10.32 VVDD112 pKa = 4.69 WIINNYY118 pKa = 9.2 VPLDD122 pKa = 3.5 VSYY125 pKa = 11.54 SDD127 pKa = 3.2 NFCYY131 pKa = 10.35 FEE133 pKa = 4.11 CLRR136 pKa = 11.84 YY137 pKa = 9.31 GHH139 pKa = 6.77 KK140 pKa = 10.52 SIAAMLIQHH149 pKa = 6.47 EE150 pKa = 4.76 SFNGSLDD157 pKa = 3.58 VLLDD161 pKa = 3.48 LLNRR165 pKa = 11.84 QEE167 pKa = 4.22 YY168 pKa = 9.73 NQFTTCVNKK177 pKa = 10.54 NNNLDD182 pKa = 3.87 EE183 pKa = 4.13 NLKK186 pKa = 10.47 NIRR189 pKa = 11.84 STLVYY194 pKa = 10.25 LVNTGDD200 pKa = 3.63 VQKK203 pKa = 10.88 VKK205 pKa = 10.88 SILTEE210 pKa = 3.96 YY211 pKa = 10.91 KK212 pKa = 8.22 STKK215 pKa = 10.1 VINTEE220 pKa = 3.92 SLSQIDD226 pKa = 3.7 ITQSTSNIVSVSDD239 pKa = 3.56 EE240 pKa = 5.13 PITVDD245 pKa = 4.64 LGVEE249 pKa = 4.21 QPVEE253 pKa = 4.11 QPTEE257 pKa = 3.95 QPTEE261 pKa = 3.86 QSTEE265 pKa = 3.91 QPTEE269 pKa = 3.8 QPTEE273 pKa = 3.86 QSTEE277 pKa = 3.88 QPTEE281 pKa = 3.65 QSTEE285 pKa = 3.88 QPTEE289 pKa = 3.62 QSTVQLTEE297 pKa = 3.73 QPTEE301 pKa = 3.78 QLTEE305 pKa = 3.88 QLTEE309 pKa = 3.85 QLTEE313 pKa = 3.92 QLTEE317 pKa = 4.05 QPVEE321 pKa = 4.17 QPVEE325 pKa = 4.14 QPVEE329 pKa = 4.1 QPTEE333 pKa = 4.01 QPVEE337 pKa = 4.08 QPTEE341 pKa = 3.85 QLTEE345 pKa = 4.04 QPVEE349 pKa = 4.16 QPTEE353 pKa = 3.9 QPIGVINPDD362 pKa = 2.97 ISIDD366 pKa = 4.12 PIQQ369 pKa = 4.23
Molecular weight: 41.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.79
IPC2_protein 3.961
IPC_protein 3.923
Toseland 3.745
ProMoST 4.024
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.732
Rodwell 3.745
Grimsley 3.656
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.113
Thurlkill 3.757
EMBOSS 3.757
Sillero 4.024
Patrickios 0.846
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A2P1EKT4|A0A2P1EKT4_9VIRU Uncharacterized protein OS=Moumouvirus australiensis OX=2109587 GN=mc_117 PE=4 SV=1
MM1 pKa = 7.23 NRR3 pKa = 11.84 NIMFARR9 pKa = 11.84 RR10 pKa = 11.84 SSKK13 pKa = 8.83 FTSIISTQGFNNMKK27 pKa = 8.3 TQYY30 pKa = 10.24 SRR32 pKa = 11.84 SIYY35 pKa = 7.84 TRR37 pKa = 11.84 RR38 pKa = 11.84 NTNINKK44 pKa = 9.56 KK45 pKa = 10.52 VIIEE49 pKa = 4.19 SLDD52 pKa = 3.37 SFMDD56 pKa = 4.43 GFTNGCLFGSVVILCYY72 pKa = 10.62 INMM75 pKa = 4.44
Molecular weight: 8.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.619
IPC_protein 10.028
Toseland 10.233
ProMoST 9.955
Dawson 10.423
Bjellqvist 10.145
Wikipedia 10.613
Rodwell 10.76
Grimsley 10.496
Solomon 10.482
Lehninger 10.452
Nozaki 10.277
DTASelect 10.116
Thurlkill 10.277
EMBOSS 10.628
Sillero 10.35
Patrickios 10.54
IPC_peptide 10.482
IPC2_peptide 9.224
IPC2.peptide.svr19 8.466
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
882
0
882
314460
64
3382
356.5
41.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.971 ± 0.082
1.805 ± 0.053
6.463 ± 0.12
5.683 ± 0.089
4.841 ± 0.069
5.062 ± 0.319
1.974 ± 0.047
10.246 ± 0.129
9.29 ± 0.122
8.351 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.865 ± 0.048
9.624 ± 0.138
3.297 ± 0.075
3.024 ± 0.059
2.95 ± 0.059
6.651 ± 0.084
4.884 ± 0.058
4.579 ± 0.066
0.825 ± 0.031
5.615 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here