Guinea pig adenovirus
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H1TG59|A0A6H1TG59_9ADEN Pre-hexon-linking protein VIII OS=Guinea pig adenovirus OX=47901 GN=L4 PE=2 SV=1
MM1 pKa = 7.9 DD2 pKa = 5.24 LCSFSVEE9 pKa = 4.04 QLGTGARR16 pKa = 11.84 SPEE19 pKa = 3.96 PFGLGVASVVFYY31 pKa = 10.82 SSEE34 pKa = 4.28 SVVVYY39 pKa = 10.8 AGDD42 pKa = 3.68 TLVVDD47 pKa = 3.67 TGVYY51 pKa = 10.26 GLLSEE56 pKa = 5.88 GYY58 pKa = 9.92 FGTLGALPGSVSGLNSCVVSGLVPGGMRR86 pKa = 11.84 HH87 pKa = 4.92 RR88 pKa = 11.84 VRR90 pKa = 11.84 VTVSNWGAHH99 pKa = 5.3 RR100 pKa = 11.84 CHH102 pKa = 6.49 IRR104 pKa = 11.84 VGSPLCMMLVHH115 pKa = 6.58 EE116 pKa = 4.75 SVVCRR121 pKa = 11.84 VEE123 pKa = 4.11 RR124 pKa = 11.84 AEE126 pKa = 4.34 GDD128 pKa = 3.69 SLPRR132 pKa = 11.84 PRR134 pKa = 11.84 DD135 pKa = 3.15 PVARR139 pKa = 11.84 RR140 pKa = 11.84 LRR142 pKa = 11.84 WEE144 pKa = 4.65 DD145 pKa = 3.39 DD146 pKa = 3.85 GEE148 pKa = 5.69 DD149 pKa = 3.98 GGSSTDD155 pKa = 3.23 TDD157 pKa = 4.07 FLEE160 pKa = 4.58 PDD162 pKa = 3.61 DD163 pKa = 4.36 RR164 pKa = 11.84 VGRR167 pKa = 11.84 PASVAATAAAARR179 pKa = 11.84 SEE181 pKa = 4.76 SEE183 pKa = 5.47 DD184 pKa = 3.51 SDD186 pKa = 3.43 ATNIDD191 pKa = 3.97 DD192 pKa = 4.03 TTTDD196 pKa = 3.37 DD197 pKa = 4.22 DD198 pKa = 4.59 EE199 pKa = 4.72 EE200 pKa = 4.86 VEE202 pKa = 4.01 EE203 pKa = 5.75 DD204 pKa = 3.56 EE205 pKa = 5.35 NEE207 pKa = 3.93 DD208 pKa = 3.47 RR209 pKa = 11.84 GGGGGGGGAVTRR221 pKa = 11.84 GDD223 pKa = 3.14 EE224 pKa = 4.36 GMDD227 pKa = 3.32 EE228 pKa = 4.1 EE229 pKa = 5.53 VVFRR233 pKa = 11.84 PEE235 pKa = 3.76 AGGEE239 pKa = 4.03 MEE241 pKa = 4.28 GQEE244 pKa = 4.1 GRR246 pKa = 11.84 TPEE249 pKa = 4.32 PEE251 pKa = 4.34 PASEE255 pKa = 3.95 AHH257 pKa = 6.89 PEE259 pKa = 3.98 PEE261 pKa = 4.69 PEE263 pKa = 4.18 RR264 pKa = 11.84 DD265 pKa = 3.16 WVDD268 pKa = 3.31 QMVPLFQSLVFQITLDD284 pKa = 3.5 GTAVKK289 pKa = 10.36 EE290 pKa = 4.37 SILGKK295 pKa = 9.64 LVRR298 pKa = 11.84 GMLDD302 pKa = 2.81 QVRR305 pKa = 11.84 NVLVFEE311 pKa = 3.86 HH312 pKa = 6.52 HH313 pKa = 6.17 MRR315 pKa = 11.84 MEE317 pKa = 4.62 GPGLSSRR324 pKa = 11.84 ARR326 pKa = 11.84 RR327 pKa = 11.84 RR328 pKa = 11.84 YY329 pKa = 9.81 RR330 pKa = 11.84 SLLDD334 pKa = 3.4 AALGEE339 pKa = 4.35 RR340 pKa = 11.84 GSARR344 pKa = 11.84 RR345 pKa = 11.84 LRR347 pKa = 11.84 LHH349 pKa = 6.58 LPEE352 pKa = 4.87 HH353 pKa = 6.39 RR354 pKa = 11.84 RR355 pKa = 11.84 AMDD358 pKa = 3.82 LGFVSMSQRR367 pKa = 11.84 RR368 pKa = 11.84 EE369 pKa = 3.86 SRR371 pKa = 11.84 WRR373 pKa = 11.84 VVCDD377 pKa = 3.19 LTVGGMRR384 pKa = 11.84 YY385 pKa = 9.75 DD386 pKa = 3.57 VQDD389 pKa = 4.06 PQDD392 pKa = 4.34 LLTEE396 pKa = 4.09 LRR398 pKa = 11.84 DD399 pKa = 3.85 TVEE402 pKa = 4.09 HH403 pKa = 5.4 YY404 pKa = 8.37 WRR406 pKa = 11.84 RR407 pKa = 11.84 RR408 pKa = 11.84 GPGVAVDD415 pKa = 3.63 VCRR418 pKa = 11.84 SPNLFF423 pKa = 3.67
Molecular weight: 46.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.791
IPC2_protein 4.736
IPC_protein 4.698
Toseland 4.558
ProMoST 4.8
Dawson 4.647
Bjellqvist 4.787
Wikipedia 4.52
Rodwell 4.546
Grimsley 4.469
Solomon 4.647
Lehninger 4.596
Nozaki 4.749
DTASelect 4.914
Thurlkill 4.558
EMBOSS 4.533
Sillero 4.825
Patrickios 4.113
IPC_peptide 4.66
IPC2_peptide 4.813
IPC2.peptide.svr19 4.792
Protein with the highest isoelectric point:
>tr|A0A6H1TF03|A0A6H1TF03_9ADEN PVI OS=Guinea pig adenovirus OX=47901 PE=4 SV=1
MM1 pKa = 7.5 TSAQKK6 pKa = 10.87 SSVSKK11 pKa = 10.56 KK12 pKa = 10.21 AALSTLSAPLARR24 pKa = 11.84 SAARR28 pKa = 11.84 RR29 pKa = 11.84 SSSWSAKK36 pKa = 6.67 TLRR39 pKa = 11.84 LLSSRR44 pKa = 11.84 VAKK47 pKa = 10.28 EE48 pKa = 3.69 PYY50 pKa = 9.7 RR51 pKa = 11.84 EE52 pKa = 4.15 LDD54 pKa = 2.94 SSLAMLRR61 pKa = 11.84 RR62 pKa = 11.84 VALRR66 pKa = 11.84 SASARR71 pKa = 11.84 SLAAMFTLTYY81 pKa = 10.54 SRR83 pKa = 11.84 ARR85 pKa = 11.84 QFQAGNTVVRR95 pKa = 11.84 SSGTTRR101 pKa = 11.84 TRR103 pKa = 11.84 QPRR106 pKa = 11.84 LQRR109 pKa = 11.84 VTASRR114 pKa = 11.84 LVHH117 pKa = 5.47 TSPRR121 pKa = 11.84 RR122 pKa = 11.84 RR123 pKa = 11.84 SLVQHH128 pKa = 5.85 SRR130 pKa = 11.84 PPRR133 pKa = 11.84 RR134 pKa = 11.84 EE135 pKa = 3.6 QKK137 pKa = 10.73 GGSTSNRR144 pKa = 11.84 RR145 pKa = 11.84 SSGGSASITNMAGRR159 pKa = 11.84 RR160 pKa = 11.84 RR161 pKa = 11.84 LSKK164 pKa = 10.88
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.877
IPC_protein 12.34
Toseland 12.501
ProMoST 12.998
Dawson 12.501
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.12
Grimsley 12.544
Solomon 12.998
Lehninger 12.896
Nozaki 12.501
DTASelect 12.501
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 11.828
IPC_peptide 12.998
IPC2_peptide 11.989
IPC2.peptide.svr19 9.13
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
31
1
32
12081
77
1111
377.5
41.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.603 ± 0.479
1.763 ± 0.32
5.769 ± 0.375
4.801 ± 0.405
3.377 ± 0.247
7.599 ± 0.59
2.988 ± 0.319
2.409 ± 0.2
2.102 ± 0.219
8.633 ± 0.46
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.763 ± 0.141
3.104 ± 0.369
7.251 ± 0.505
3.551 ± 0.274
9.56 ± 0.503
6.829 ± 0.437
5.827 ± 0.323
8.054 ± 0.482
1.068 ± 0.131
2.947 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here