Carrot red leaf virus associated RNA

Taxonomy: Viruses; Riboviria; Virus-associated RNAs

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2MW35|A0A6H2MW35_9VIRU Uncharacterized protein OS=Carrot red leaf virus associated RNA OX=1425368 PE=4 SV=1
MM1 pKa = 8.07DD2 pKa = 5.42RR3 pKa = 11.84LNDD6 pKa = 3.64EE7 pKa = 4.48CHH9 pKa = 6.31CLAVRR14 pKa = 11.84GVPLSKK20 pKa = 10.61ASPSCAMLGIYY31 pKa = 9.75EE32 pKa = 4.29IVDD35 pKa = 3.22GGNYY39 pKa = 9.56RR40 pKa = 11.84GGYY43 pKa = 8.76APSYY47 pKa = 8.83YY48 pKa = 9.89PGVKK52 pKa = 9.7YY53 pKa = 10.15PHH55 pKa = 7.16DD56 pKa = 3.9TTPKK60 pKa = 9.66PEE62 pKa = 3.71WGRR65 pKa = 3.57

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2MW46|A0A6H2MW46_9VIRU RNA-directed RNA polymerase OS=Carrot red leaf virus associated RNA OX=1425368 PE=4 SV=1
MM1 pKa = 7.59SNLNAVTLMGTQPAVSKK18 pKa = 10.89LVGLLPKK25 pKa = 10.47LSSRR29 pKa = 11.84LIDD32 pKa = 3.7AAKK35 pKa = 10.66VPATCGLIVAAGLGLYY51 pKa = 9.97AYY53 pKa = 10.2RR54 pKa = 11.84QVCINKK60 pKa = 9.95LEE62 pKa = 4.13LVRR65 pKa = 11.84EE66 pKa = 4.23NTTPRR71 pKa = 11.84PTDD74 pKa = 3.99CIEE77 pKa = 4.45DD78 pKa = 3.68VLEE81 pKa = 4.31VEE83 pKa = 4.6EE84 pKa = 5.42DD85 pKa = 3.33AAADD89 pKa = 3.58LTLAKK94 pKa = 9.73IAKK97 pKa = 7.33EE98 pKa = 3.91AAMEE102 pKa = 4.17AALNPGEE109 pKa = 4.03VMEE112 pKa = 4.78RR113 pKa = 11.84VRR115 pKa = 11.84EE116 pKa = 3.96AAKK119 pKa = 10.55INAQPSTNPLAKK131 pKa = 9.86RR132 pKa = 11.84RR133 pKa = 11.84TRR135 pKa = 11.84LRR137 pKa = 11.84PNKK140 pKa = 8.1TGQFIRR146 pKa = 11.84VLRR149 pKa = 11.84AEE151 pKa = 4.97IKK153 pKa = 10.39SQMGTPTITAANEE166 pKa = 3.42AVIRR170 pKa = 11.84HH171 pKa = 6.13MISKK175 pKa = 9.79FCASHH180 pKa = 6.98NIRR183 pKa = 11.84TTSYY187 pKa = 8.54AHH189 pKa = 7.02LISRR193 pKa = 11.84VVRR196 pKa = 11.84EE197 pKa = 3.94VMTPYY202 pKa = 10.43PGDD205 pKa = 3.51AEE207 pKa = 4.21EE208 pKa = 4.36VEE210 pKa = 4.51RR211 pKa = 11.84ANSVVNRR218 pKa = 11.84IHH220 pKa = 6.21NWLVQYY226 pKa = 10.79RR227 pKa = 11.84KK228 pKa = 10.18

Molecular weight: 25.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1050

65

757

350.0

38.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.952 ± 1.754

2.095 ± 0.635

4.571 ± 0.914

6.286 ± 0.633

3.143 ± 1.364

6.0 ± 1.739

1.619 ± 0.38

5.238 ± 0.639

5.714 ± 0.363

8.476 ± 0.636

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.323

4.476 ± 0.617

5.524 ± 1.313

2.19 ± 0.564

7.524 ± 0.47

6.762 ± 0.903

6.762 ± 0.922

7.905 ± 0.552

1.238 ± 0.358

2.952 ± 1.578
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski