Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIMB 8403 / VKM B-1763) (Maridesulfovibrio salexigens)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Maridesulfovibrio; Maridesulfovibrio salexigens

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3807 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6BZY2|C6BZY2_DESAD Extracellular solute-binding protein family 3 OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIMB 8403 / VKM B-1763) OX=526222 GN=Desal_2801 PE=3 SV=1
MM1 pKa = 7.55SLTNTLSIGQRR12 pKa = 11.84ALANAQVSINTTSNNIANSEE32 pKa = 4.23TEE34 pKa = 4.34GYY36 pKa = 9.81QRR38 pKa = 11.84ADD40 pKa = 2.9AVYY43 pKa = 10.67DD44 pKa = 3.4SLGNINSYY52 pKa = 11.05GNSLGTGADD61 pKa = 2.85IVAIRR66 pKa = 11.84ANWDD70 pKa = 3.35RR71 pKa = 11.84FIEE74 pKa = 4.17KK75 pKa = 10.13QFLIALGSLSCSEE88 pKa = 4.19AQLGYY93 pKa = 10.17LSQMDD98 pKa = 3.87SVFNQSEE105 pKa = 4.38EE106 pKa = 3.97QGLAAAQDD114 pKa = 3.97EE115 pKa = 5.36FLSAWNGLSTYY126 pKa = 9.76PDD128 pKa = 3.28SLAEE132 pKa = 4.27RR133 pKa = 11.84EE134 pKa = 4.27DD135 pKa = 3.83LLGEE139 pKa = 4.32AEE141 pKa = 4.17SLLYY145 pKa = 10.62GLNSSYY151 pKa = 11.48SEE153 pKa = 4.17LRR155 pKa = 11.84DD156 pKa = 3.17MSASVEE162 pKa = 4.18SEE164 pKa = 3.55IVDD167 pKa = 3.66QVNTANEE174 pKa = 4.64LIDD177 pKa = 5.27SIGLLNEE184 pKa = 4.21QISANPDD191 pKa = 3.01NYY193 pKa = 10.81EE194 pKa = 3.94LVASRR199 pKa = 11.84DD200 pKa = 3.48QAIRR204 pKa = 11.84EE205 pKa = 4.1LDD207 pKa = 3.44EE208 pKa = 5.18LIGVEE213 pKa = 4.13VLSYY217 pKa = 11.3EE218 pKa = 4.42DD219 pKa = 4.74GSTKK223 pKa = 9.95IYY225 pKa = 10.61TEE227 pKa = 4.01TGHH230 pKa = 6.73PLVEE234 pKa = 4.66GEE236 pKa = 4.41EE237 pKa = 4.24THH239 pKa = 6.56HH240 pKa = 6.96LAVAYY245 pKa = 10.36DD246 pKa = 4.39DD247 pKa = 4.71DD248 pKa = 4.19TSKK251 pKa = 9.73TGLFWEE257 pKa = 4.7NGSGGLVDD265 pKa = 4.12ITPMSDD271 pKa = 2.94EE272 pKa = 4.66SGDD275 pKa = 3.77AVSGRR280 pKa = 11.84ITGGSIAGLFITRR293 pKa = 11.84DD294 pKa = 3.31EE295 pKa = 5.15HH296 pKa = 6.48IQPTLDD302 pKa = 4.06RR303 pKa = 11.84LDD305 pKa = 4.0EE306 pKa = 4.09YY307 pKa = 11.41AAALIWEE314 pKa = 4.61TNRR317 pKa = 11.84AHH319 pKa = 5.95SQGAGLEE326 pKa = 3.96PHH328 pKa = 6.33TAVEE332 pKa = 4.2GTYY335 pKa = 10.67GVEE338 pKa = 4.15DD339 pKa = 3.4QTAALSDD346 pKa = 3.66SGLDD350 pKa = 3.47FEE352 pKa = 6.28DD353 pKa = 5.9RR354 pKa = 11.84ITSGEE359 pKa = 4.18FTIHH363 pKa = 6.68TYY365 pKa = 10.8DD366 pKa = 4.0ADD368 pKa = 4.24GNPEE372 pKa = 3.69ASITISIDD380 pKa = 3.13PSTDD384 pKa = 3.15SLDD387 pKa = 5.4DD388 pKa = 3.24IVSNINASLGGSLTASVNADD408 pKa = 3.18GEE410 pKa = 4.38LAIEE414 pKa = 4.18AVGDD418 pKa = 3.65SSFEE422 pKa = 4.0FGEE425 pKa = 4.45DD426 pKa = 3.02SSGFLAAAGINTFFEE441 pKa = 5.0GSSAGDD447 pKa = 3.15IAVNNYY453 pKa = 8.3VASNPSHH460 pKa = 6.81LNAGEE465 pKa = 4.13VGSDD469 pKa = 3.28GTVASGSNSTAQSINDD485 pKa = 3.86LLSRR489 pKa = 11.84DD490 pKa = 3.57VSIGEE495 pKa = 4.25GANATSATLTEE506 pKa = 4.31YY507 pKa = 11.12LSAIVSDD514 pKa = 3.87VGAAASTMEE523 pKa = 4.16TRR525 pKa = 11.84VTCDD529 pKa = 2.58TAAAQIYY536 pKa = 10.31AEE538 pKa = 4.16QQEE541 pKa = 4.89SVSGVNVDD549 pKa = 3.78EE550 pKa = 4.55EE551 pKa = 4.69LVNLTRR557 pKa = 11.84HH558 pKa = 4.25QQQYY562 pKa = 9.48EE563 pKa = 4.1AACQIISVTRR573 pKa = 11.84DD574 pKa = 3.12MIDD577 pKa = 3.05TVLGIVV583 pKa = 3.54

Molecular weight: 61.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6BXY8|C6BXY8_DESAD Metallo-beta-lactamase family protein OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIMB 8403 / VKM B-1763) OX=526222 GN=Desal_2462 PE=4 SV=1
MM1 pKa = 6.98STLARR6 pKa = 11.84LALFGAIGTLLFLPLVVKK24 pKa = 10.41RR25 pKa = 11.84KK26 pKa = 8.14HH27 pKa = 4.72RR28 pKa = 11.84HH29 pKa = 3.79RR30 pKa = 11.84HH31 pKa = 3.83RR32 pKa = 11.84HH33 pKa = 4.51GSPVLVRR40 pKa = 11.84AGGSGGKK47 pKa = 9.6KK48 pKa = 9.23GRR50 pKa = 11.84KK51 pKa = 8.31

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3807

0

3807

1264146

31

2646

332.1

36.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.24 ± 0.04

1.335 ± 0.017

5.646 ± 0.033

7.084 ± 0.037

4.367 ± 0.025

7.518 ± 0.037

1.858 ± 0.017

6.598 ± 0.033

6.271 ± 0.036

9.818 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.967 ± 0.02

3.907 ± 0.026

4.159 ± 0.029

3.129 ± 0.021

4.81 ± 0.033

6.396 ± 0.032

4.938 ± 0.031

6.952 ± 0.033

1.062 ± 0.016

2.942 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski