Oscillibacter sp. KLE 1745

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Oscillibacter; unclassified Oscillibacter

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4287 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2QRF8|U2QRF8_9FIRM AAA domain-containing protein OS=Oscillibacter sp. KLE 1745 OX=1226323 GN=HMPREF1546_03487 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.55KK3 pKa = 9.03MLSMLLALILVFSLVGCSGSSGDD26 pKa = 5.07SGTADD31 pKa = 2.96TGGDD35 pKa = 3.16SSAAEE40 pKa = 3.97EE41 pKa = 4.25SDD43 pKa = 3.82VIKK46 pKa = 10.67IGLYY50 pKa = 9.7TPLTGTSALVGTQEE64 pKa = 4.58QMGVDD69 pKa = 4.06LAVKK73 pKa = 9.69QLNEE77 pKa = 3.92AGGVNGKK84 pKa = 8.56QFSVIAYY91 pKa = 8.63DD92 pKa = 3.61DD93 pKa = 3.48QFNAEE98 pKa = 4.18TAVKK102 pKa = 10.28VVTRR106 pKa = 11.84LTQTDD111 pKa = 3.62NVDD114 pKa = 4.05AIIGSMSSANILATADD130 pKa = 3.25IVEE133 pKa = 4.19QAQVLEE139 pKa = 4.64IGCGTSPTWTNAGYY153 pKa = 10.34EE154 pKa = 4.17YY155 pKa = 11.04VFRR158 pKa = 11.84GTQNAASFNTGIVEE172 pKa = 4.19LMTRR176 pKa = 11.84MGVTRR181 pKa = 11.84LGTMVSSTEE190 pKa = 3.91YY191 pKa = 10.02ATTGWAEE198 pKa = 4.25VKK200 pKa = 10.23EE201 pKa = 4.11QLADD205 pKa = 3.41TDD207 pKa = 3.81IEE209 pKa = 4.5IVLEE213 pKa = 4.06TDD215 pKa = 3.45YY216 pKa = 10.94MAGDD220 pKa = 3.76TDD222 pKa = 3.94FTGQITRR229 pKa = 11.84LLNADD234 pKa = 3.56LDD236 pKa = 4.82GILLYY241 pKa = 10.77GATEE245 pKa = 4.69DD246 pKa = 4.43YY247 pKa = 11.18GIEE250 pKa = 4.2CKK252 pKa = 8.43QLRR255 pKa = 11.84QLGYY259 pKa = 8.95EE260 pKa = 4.31GYY262 pKa = 9.75IYY264 pKa = 10.74GSEE267 pKa = 4.05TFAATDD273 pKa = 3.48VRR275 pKa = 11.84EE276 pKa = 4.34VASDD280 pKa = 3.39AANGVLFACGYY291 pKa = 8.18VIPDD295 pKa = 4.88AIEE298 pKa = 4.18DD299 pKa = 3.69AATEE303 pKa = 4.11EE304 pKa = 4.2EE305 pKa = 4.67KK306 pKa = 11.19AFLEE310 pKa = 4.44AFVEE314 pKa = 5.26EE315 pKa = 4.59YY316 pKa = 11.54GEE318 pKa = 4.31MPVSDD323 pKa = 3.65TAYY326 pKa = 10.22RR327 pKa = 11.84GYY329 pKa = 11.28DD330 pKa = 3.42SMMLLAKK337 pKa = 10.39VFEE340 pKa = 4.62TAEE343 pKa = 4.19SMEE346 pKa = 4.4GPDD349 pKa = 5.03LRR351 pKa = 11.84EE352 pKa = 3.73ALLNVDD358 pKa = 3.41YY359 pKa = 10.83TGIGGKK365 pKa = 9.45FDD367 pKa = 3.92YY368 pKa = 10.94SDD370 pKa = 3.73GSGDD374 pKa = 3.66GLEE377 pKa = 4.4GCNLYY382 pKa = 10.94AIVDD386 pKa = 4.16GKK388 pKa = 10.37NVPFEE393 pKa = 4.19TFLADD398 pKa = 5.26LNNQQ402 pKa = 3.21

Molecular weight: 42.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2R0V6|U2R0V6_9FIRM Putative anaerobic ribonucleoside-triphosphate reductase activating protein (Fragment) OS=Oscillibacter sp. KLE 1745 OX=1226323 GN=HMPREF1546_02791 PE=4 SV=1
MM1 pKa = 7.73FFRR4 pKa = 11.84HH5 pKa = 4.4WRR7 pKa = 11.84KK8 pKa = 9.3NRR10 pKa = 11.84GSRR13 pKa = 11.84RR14 pKa = 11.84FPLAPLSRR22 pKa = 11.84MMRR25 pKa = 11.84GRR27 pKa = 11.84WKK29 pKa = 10.12YY30 pKa = 7.26QWRR33 pKa = 11.84LLPLFFRR40 pKa = 11.84MKK42 pKa = 10.58VSLNN46 pKa = 3.41

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4287

0

4287

1076406

26

3491

251.1

27.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.886 ± 0.063

1.689 ± 0.019

5.579 ± 0.043

6.813 ± 0.038

3.818 ± 0.028

7.957 ± 0.038

1.84 ± 0.02

5.44 ± 0.037

4.529 ± 0.034

9.904 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.02

3.128 ± 0.028

4.305 ± 0.024

3.569 ± 0.024

6.116 ± 0.05

5.432 ± 0.034

5.682 ± 0.046

7.016 ± 0.037

1.166 ± 0.016

3.397 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski