Clostridiales bacterium S5-A14a

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; unclassified Clostridiales Family XIII. Incertae Sedis

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095XHI1|A0A095XHI1_9FIRM Adenylate kinase OS=Clostridiales bacterium S5-A14a OX=1230734 GN=adk PE=3 SV=1
MM1 pKa = 7.25KK2 pKa = 10.02RR3 pKa = 11.84YY4 pKa = 9.59RR5 pKa = 11.84FKK7 pKa = 11.08KK8 pKa = 8.34VLLVIACFSLVIVMAACGSSGSGGGGSHH36 pKa = 7.1ASISTDD42 pKa = 3.01PDD44 pKa = 3.44NPSEE48 pKa = 4.13LTFPEE53 pKa = 4.39TNEE56 pKa = 4.72DD57 pKa = 3.08GDD59 pKa = 4.07VYY61 pKa = 11.02YY62 pKa = 10.93QSIYY66 pKa = 10.86NSTVDD71 pKa = 5.42DD72 pKa = 4.59PDD74 pKa = 5.14AEE76 pKa = 4.69PGTIYY81 pKa = 10.82GCYY84 pKa = 9.56QGTLTDD90 pKa = 4.09GDD92 pKa = 3.93TMTFVASKK100 pKa = 9.56PQLPDD105 pKa = 4.8AIAQKK110 pKa = 10.76LDD112 pKa = 3.67EE113 pKa = 5.04NGLKK117 pKa = 10.63YY118 pKa = 10.06FVISTMRR125 pKa = 11.84GDD127 pKa = 3.41IDD129 pKa = 3.62YY130 pKa = 10.29TNARR134 pKa = 11.84SKK136 pKa = 11.2LYY138 pKa = 10.16IFKK141 pKa = 10.62DD142 pKa = 3.26DD143 pKa = 4.06ASYY146 pKa = 8.85DD147 pKa = 3.88TISRR151 pKa = 11.84DD152 pKa = 3.28QEE154 pKa = 4.36TVKK157 pKa = 10.68VFGRR161 pKa = 11.84YY162 pKa = 9.04IGISLGTEE170 pKa = 4.07DD171 pKa = 4.73NYY173 pKa = 11.68SDD175 pKa = 4.58AGSGCIDD182 pKa = 3.63EE183 pKa = 4.93NFSIYY188 pKa = 9.88EE189 pKa = 4.06ASDD192 pKa = 3.32EE193 pKa = 4.03EE194 pKa = 4.38LALGIIDD201 pKa = 4.2YY202 pKa = 10.35LISHH206 pKa = 7.41

Molecular weight: 22.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095ZNI1|A0A095ZNI1_9FIRM Uncharacterized protein OS=Clostridiales bacterium S5-A14a OX=1230734 GN=HMPREF1635_00560 PE=4 SV=1
MM1 pKa = 7.84AKK3 pKa = 10.54NKK5 pKa = 8.97MKK7 pKa = 10.15SHH9 pKa = 6.8RR10 pKa = 11.84GAKK13 pKa = 9.39KK14 pKa = 9.75RR15 pKa = 11.84LKK17 pKa = 9.75LTGSGKK23 pKa = 9.9IKK25 pKa = 10.24RR26 pKa = 11.84FKK28 pKa = 10.71AFKK31 pKa = 10.59SHH33 pKa = 6.94ILTKK37 pKa = 10.4KK38 pKa = 7.83AAKK41 pKa = 9.74RR42 pKa = 11.84KK43 pKa = 8.98RR44 pKa = 11.84GLRR47 pKa = 11.84KK48 pKa = 8.56STIVTSADD56 pKa = 3.24HH57 pKa = 6.71KK58 pKa = 11.07RR59 pKa = 11.84MLRR62 pKa = 11.84AMGKK66 pKa = 9.74

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1151

0

1151

379274

37

2432

329.5

36.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.927 ± 0.081

1.139 ± 0.029

6.186 ± 0.061

7.425 ± 0.078

4.264 ± 0.051

7.061 ± 0.062

1.598 ± 0.029

8.567 ± 0.068

8.126 ± 0.08

8.607 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.016 ± 0.045

4.91 ± 0.063

3.191 ± 0.039

2.497 ± 0.032

4.094 ± 0.052

6.264 ± 0.053

4.978 ± 0.048

6.745 ± 0.064

0.715 ± 0.02

3.687 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski