Phlebia centrifuga
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13628 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R6S5A0|A0A2R6S5A0_9APHY Uncharacterized protein OS=Phlebia centrifuga OX=98765 GN=PHLCEN_2v674 PE=4 SV=1
MM1 pKa = 6.85 STEE4 pKa = 4.14 TTSLFSLARR13 pKa = 11.84 NKK15 pKa = 10.16 LQSVVGGGVKK25 pKa = 10.27 DD26 pKa = 3.34 SCSLHH31 pKa = 6.14 RR32 pKa = 11.84 WVLLKK37 pKa = 10.98 NSIIRR42 pKa = 11.84 SHH44 pKa = 7.15 PPLAAHH50 pKa = 7.34 DD51 pKa = 4.12 ATDD54 pKa = 3.5 EE55 pKa = 4.18 ADD57 pKa = 3.35 ADD59 pKa = 3.62 SVYY62 pKa = 10.63 RR63 pKa = 11.84 HH64 pKa = 5.92 EE65 pKa = 4.62 EE66 pKa = 4.09 EE67 pKa = 4.24 EE68 pKa = 4.2 SLEE71 pKa = 4.05 EE72 pKa = 4.54 DD73 pKa = 3.67 EE74 pKa = 5.6 QDD76 pKa = 2.92 TFMFPDD82 pKa = 3.68 PHH84 pKa = 7.52 YY85 pKa = 10.13 LLPDD89 pKa = 3.48 EE90 pKa = 4.98 TPAAGIDD97 pKa = 3.36 EE98 pKa = 4.41 RR99 pKa = 11.84 QWLDD103 pKa = 3.16 SLLEE107 pKa = 4.12 GLEE110 pKa = 4.3 DD111 pKa = 6.16 DD112 pKa = 5.49 EE113 pKa = 7.72 DD114 pKa = 5.35 DD115 pKa = 6.05 DD116 pKa = 4.85 YY117 pKa = 12.3 SKK119 pKa = 11.74 LDD121 pKa = 3.7 GQLSALPTLATDD133 pKa = 4.88 DD134 pKa = 3.76 VAEE137 pKa = 4.61 PLSPLYY143 pKa = 11.14 SPMSSSDD150 pKa = 3.76 DD151 pKa = 3.9 LADD154 pKa = 3.35 QSNYY158 pKa = 9.4 FSYY161 pKa = 10.42 PMPYY165 pKa = 9.65 PPPHH169 pKa = 7.11 PPLIPSWFEE178 pKa = 3.42 LQAPSDD184 pKa = 4.03 SALTSPVILCDD195 pKa = 3.64 NPLPYY200 pKa = 10.49 YY201 pKa = 10.78 AVDD204 pKa = 4.12 DD205 pKa = 5.09 LEE207 pKa = 4.96 DD208 pKa = 4.56 LPVPDD213 pKa = 5.89 AIEE216 pKa = 4.42 DD217 pKa = 3.94 TSDD220 pKa = 4.1 DD221 pKa = 3.89 EE222 pKa = 5.66 SDD224 pKa = 3.84 APSTPSATSTSSLAPINSSSISTPPGRR251 pKa = 11.84 TRR253 pKa = 11.84 FLSDD257 pKa = 3.05 PQVYY261 pKa = 10.34 VDD263 pKa = 3.9 TDD265 pKa = 3.51 DD266 pKa = 3.36 SHH268 pKa = 7.36 FYY270 pKa = 10.54 PFEE273 pKa = 5.14 LDD275 pKa = 3.53 PLPFPGPYY283 pKa = 9.43 HH284 pKa = 6.95 AGVV287 pKa = 3.25
Molecular weight: 31.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.706
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 0.985
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|A0A2R6NYK5|A0A2R6NYK5_9APHY Uncharacterized protein OS=Phlebia centrifuga OX=98765 GN=PHLCEN_2v6923 PE=4 SV=1
MM1 pKa = 7.49 AVIAKK6 pKa = 9.61 IALAATGLARR16 pKa = 11.84 SSKK19 pKa = 9.99 PGRR22 pKa = 11.84 ILNRR26 pKa = 11.84 VVNQIARR33 pKa = 11.84 MGVLVRR39 pKa = 11.84 VLTWPKK45 pKa = 9.43 YY46 pKa = 8.21 PRR48 pKa = 11.84 SGRR51 pKa = 11.84 PLSRR55 pKa = 11.84 LKK57 pKa = 10.93 AA58 pKa = 3.68
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.994
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.208
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.945
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.057
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13628
0
13628
5081813
49
3910
372.9
41.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.38 ± 0.02
1.163 ± 0.008
5.789 ± 0.014
6.184 ± 0.022
3.598 ± 0.014
6.389 ± 0.02
2.532 ± 0.011
4.857 ± 0.015
4.612 ± 0.02
9.113 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.143 ± 0.009
3.421 ± 0.012
6.565 ± 0.024
3.788 ± 0.014
6.209 ± 0.018
8.851 ± 0.029
6.044 ± 0.013
6.359 ± 0.018
1.403 ± 0.007
2.6 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here