Stenotrophomonas phage Mendera

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Menderavirus; Stenotrophomonas virus Mendera

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PJ44|A0A5P8PJ44_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Mendera OX=2650877 GN=CPT_Mendera_236 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.59PFEE5 pKa = 4.64EE6 pKa = 4.49YY7 pKa = 10.95AVIDD11 pKa = 4.61DD12 pKa = 5.38YY13 pKa = 12.09GDD15 pKa = 3.46DD16 pKa = 3.84WEE18 pKa = 4.88EE19 pKa = 4.17GPIAFSGDD27 pKa = 3.19TFEE30 pKa = 5.86QCAEE34 pKa = 3.76WAEE37 pKa = 4.24SEE39 pKa = 4.46NYY41 pKa = 9.89HH42 pKa = 6.09SASIVGILEE51 pKa = 3.38NGRR54 pKa = 11.84RR55 pKa = 11.84VKK57 pKa = 10.36IVV59 pKa = 2.81

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PJA5|A0A5P8PJA5_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Mendera OX=2650877 GN=CPT_Mendera_281 PE=4 SV=1
MM1 pKa = 7.29ATVKK5 pKa = 10.41INPTRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.26VQVKK16 pKa = 9.56PGTSTEE22 pKa = 3.95QTQLTKK28 pKa = 10.74KK29 pKa = 9.52ATIEE33 pKa = 4.07AKK35 pKa = 10.46VKK37 pKa = 9.76SAEE40 pKa = 3.96RR41 pKa = 11.84GADD44 pKa = 3.23AKK46 pKa = 10.65ALKK49 pKa = 9.36WFYY52 pKa = 11.3DD53 pKa = 3.78LVGSKK58 pKa = 9.67IKK60 pKa = 10.5RR61 pKa = 11.84GNVPDD66 pKa = 3.38QAFAPRR72 pKa = 11.84RR73 pKa = 11.84GRR75 pKa = 11.84NVFIGGMFVYY85 pKa = 9.86GYY87 pKa = 10.35DD88 pKa = 3.82PKK90 pKa = 11.21HH91 pKa = 6.84KK92 pKa = 9.58DD93 pKa = 3.12TLPWYY98 pKa = 7.5DD99 pKa = 4.12TLPCVIPIEE108 pKa = 4.49FYY110 pKa = 9.97PDD112 pKa = 2.51GWLGLNLHH120 pKa = 6.33YY121 pKa = 10.53LPPILRR127 pKa = 11.84AKK129 pKa = 10.76LLDD132 pKa = 3.3TLMTYY137 pKa = 10.0RR138 pKa = 11.84RR139 pKa = 11.84RR140 pKa = 11.84AGDD143 pKa = 3.03DD144 pKa = 2.92RR145 pKa = 11.84AYY147 pKa = 10.38MKK149 pKa = 10.66LSYY152 pKa = 11.19GMLKK156 pKa = 10.44GLAVHH161 pKa = 6.78PSVAPCIKK169 pKa = 10.12RR170 pKa = 11.84YY171 pKa = 8.99LANHH175 pKa = 5.14VTTPVIRR182 pKa = 11.84IDD184 pKa = 3.22RR185 pKa = 11.84MYY187 pKa = 9.68WDD189 pKa = 4.21NVALLPLQQFKK200 pKa = 10.56KK201 pKa = 10.74ARR203 pKa = 11.84ASQVWAATRR212 pKa = 11.84PKK214 pKa = 9.76TRR216 pKa = 11.84RR217 pKa = 11.84SRR219 pKa = 11.84KK220 pKa = 9.39KK221 pKa = 9.13VV222 pKa = 3.1

Molecular weight: 25.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

286

0

286

50099

29

1931

175.2

19.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.733 ± 0.215

1.1 ± 0.069

6.563 ± 0.122

6.872 ± 0.273

4.19 ± 0.1

6.623 ± 0.163

1.768 ± 0.103

5.96 ± 0.105

6.092 ± 0.214

8.046 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.842 ± 0.096

4.727 ± 0.133

4.09 ± 0.128

3.828 ± 0.101

5.302 ± 0.143

5.91 ± 0.159

6.048 ± 0.231

6.753 ± 0.138

1.699 ± 0.077

3.854 ± 0.106
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski