Amino acid dipepetide frequency for Bacillus methanolicus (strain MGA3 / ATCC 53907)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.947AlaAla: 5.947 ± 0.112	0.558AlaCys: 0.558 ± 0.025	3.198AlaAsp: 3.198 ± 0.067	4.758AlaGlu: 4.758 ± 0.073	3.196AlaPhe: 3.196 ± 0.06	5.215AlaGly: 5.215 ± 0.089	1.21AlaHis: 1.21 ± 0.036	5.711AlaIle: 5.711 ± 0.084	5.278AlaLys: 5.278 ± 0.095	6.901AlaLeu: 6.901 ± 0.097	1.874AlaMet: 1.874 ± 0.047	2.732AlaAsn: 2.732 ± 0.052	1.922AlaPro: 1.922 ± 0.046	2.088AlaGln: 2.088 ± 0.052	2.798AlaArg: 2.798 ± 0.071	3.773AlaSer: 3.773 ± 0.074	2.997AlaThr: 2.997 ± 0.052	5.513AlaVal: 5.513 ± 0.08	0.538AlaTrp: 0.538 ± 0.027	2.228AlaTyr: 2.228 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.456CysAla: 0.456 ± 0.025	0.097CysCys: 0.097 ± 0.011	0.376CysAsp: 0.376 ± 0.023	0.496CysGlu: 0.496 ± 0.024	0.344CysPhe: 0.344 ± 0.021	0.708CysGly: 0.708 ± 0.027	0.185CysHis: 0.185 ± 0.014	0.551CysIle: 0.551 ± 0.024	0.369CysLys: 0.369 ± 0.021	0.687CysLeu: 0.687 ± 0.026	0.165CysMet: 0.165 ± 0.013	0.317CysAsn: 0.317 ± 0.021	0.359CysPro: 0.359 ± 0.022	0.245CysGln: 0.245 ± 0.017	0.312CysArg: 0.312 ± 0.015	0.563CysSer: 0.563 ± 0.026	0.389CysThr: 0.389 ± 0.021	0.36CysVal: 0.36 ± 0.02	0.071CysTrp: 0.071 ± 0.009	0.27CysTyr: 0.27 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.02AspAla: 3.02 ± 0.07	0.398AspCys: 0.398 ± 0.024	2.28AspAsp: 2.28 ± 0.05	4.213AspGlu: 4.213 ± 0.081	2.401AspPhe: 2.401 ± 0.05	3.143AspGly: 3.143 ± 0.058	1.199AspHis: 1.199 ± 0.041	4.093AspIle: 4.093 ± 0.074	3.149AspLys: 3.149 ± 0.061	4.967AspLeu: 4.967 ± 0.082	1.17AspMet: 1.17 ± 0.034	1.679AspAsn: 1.679 ± 0.041	2.152AspPro: 2.152 ± 0.046	1.88AspGln: 1.88 ± 0.043	2.305AspArg: 2.305 ± 0.045	2.402AspSer: 2.402 ± 0.053	2.058AspThr: 2.058 ± 0.043	3.354AspVal: 3.354 ± 0.067	0.562AspTrp: 0.562 ± 0.024	1.92AspTyr: 1.92 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	5.126GluAla: 5.126 ± 0.084	0.396GluCys: 0.396 ± 0.022	3.454GluAsp: 3.454 ± 0.066	6.976GluGlu: 6.976 ± 0.12	2.811GluPhe: 2.811 ± 0.057	4.279GluGly: 4.279 ± 0.072	1.528GluHis: 1.528 ± 0.042	6.34GluIle: 6.34 ± 0.095	7.953GluLys: 7.953 ± 0.111	7.304GluLeu: 7.304 ± 0.101	2.27GluMet: 2.27 ± 0.054	4.008GluAsn: 4.008 ± 0.068	2.005GluPro: 2.005 ± 0.047	3.115GluGln: 3.115 ± 0.073	3.761GluArg: 3.761 ± 0.08	3.31GluSer: 3.31 ± 0.072	3.76GluThr: 3.76 ± 0.062	4.71GluVal: 4.71 ± 0.082	0.835GluTrp: 0.835 ± 0.03	2.286GluTyr: 2.286 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	3.08PheAla: 3.08 ± 0.061	0.371PheCys: 0.371 ± 0.021	2.309PheAsp: 2.309 ± 0.052	3.034PheGlu: 3.034 ± 0.059	2.549PhePhe: 2.549 ± 0.066	3.452PheGly: 3.452 ± 0.073	1.157PheHis: 1.157 ± 0.039	3.976PheIle: 3.976 ± 0.085	2.521PheLys: 2.521 ± 0.056	5.051PheLeu: 5.051 ± 0.089	1.186PheMet: 1.186 ± 0.037	1.792PheAsn: 1.792 ± 0.043	1.799PhePro: 1.799 ± 0.043	1.709PheGln: 1.709 ± 0.045	1.763PheArg: 1.763 ± 0.048	3.308PheSer: 3.308 ± 0.069	2.35PheThr: 2.35 ± 0.055	3.156PheVal: 3.156 ± 0.069	0.485PheTrp: 0.485 ± 0.024	1.743PheTyr: 1.743 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	4.739GlyAla: 4.739 ± 0.09	0.664GlyCys: 0.664 ± 0.027	2.949GlyAsp: 2.949 ± 0.062	4.321GlyGlu: 4.321 ± 0.072	3.428GlyPhe: 3.428 ± 0.065	4.764GlyGly: 4.764 ± 0.09	1.403GlyHis: 1.403 ± 0.045	6.231GlyIle: 6.231 ± 0.092	5.598GlyLys: 5.598 ± 0.075	6.407GlyLeu: 6.407 ± 0.09	2.014GlyMet: 2.014 ± 0.048	2.802GlyAsn: 2.802 ± 0.063	1.893GlyPro: 1.893 ± 0.044	2.141GlyGln: 2.141 ± 0.049	2.824GlyArg: 2.824 ± 0.059	3.743GlySer: 3.743 ± 0.08	4.024GlyThr: 4.024 ± 0.078	4.799GlyVal: 4.799 ± 0.082	0.75GlyTrp: 0.75 ± 0.031	2.851GlyTyr: 2.851 ± 0.067	0.0GlyXaa: 0.0 ± 0.0
His	1.218HisAla: 1.218 ± 0.033	0.208HisCys: 0.208 ± 0.017	0.997HisAsp: 0.997 ± 0.033	1.36HisGlu: 1.36 ± 0.037	1.108HisPhe: 1.108 ± 0.039	1.457HisGly: 1.457 ± 0.041	0.707HisHis: 0.707 ± 0.03	1.535HisIle: 1.535 ± 0.039	1.105HisLys: 1.105 ± 0.039	2.226HisLeu: 2.226 ± 0.061	0.473HisMet: 0.473 ± 0.021	0.755HisAsn: 0.755 ± 0.029	1.186HisPro: 1.186 ± 0.041	0.807HisGln: 0.807 ± 0.027	0.888HisArg: 0.888 ± 0.038	1.261HisSer: 1.261 ± 0.039	0.964HisThr: 0.964 ± 0.035	1.479HisVal: 1.479 ± 0.042	0.239HisTrp: 0.239 ± 0.015	0.854HisTyr: 0.854 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	6.003IleAla: 6.003 ± 0.09	0.678IleCys: 0.678 ± 0.027	4.301IleAsp: 4.301 ± 0.07	6.181IleGlu: 6.181 ± 0.092	3.549IlePhe: 3.549 ± 0.073	6.121IleGly: 6.121 ± 0.099	1.758IleHis: 1.758 ± 0.049	6.372IleIle: 6.372 ± 0.101	5.474IleLys: 5.474 ± 0.081	7.459IleLeu: 7.459 ± 0.11	1.812IleMet: 1.812 ± 0.047	3.467IleAsn: 3.467 ± 0.062	3.555IlePro: 3.555 ± 0.059	2.874IleGln: 2.874 ± 0.061	3.526IleArg: 3.526 ± 0.061	5.224IleSer: 5.224 ± 0.086	4.289IleThr: 4.289 ± 0.074	5.775IleVal: 5.775 ± 0.078	0.721IleTrp: 0.721 ± 0.034	2.579IleTyr: 2.579 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	5.211LysAla: 5.211 ± 0.08	0.359LysCys: 0.359 ± 0.02	4.109LysAsp: 4.109 ± 0.073	7.797LysGlu: 7.797 ± 0.094	2.307LysPhe: 2.307 ± 0.047	4.935LysGly: 4.935 ± 0.091	1.345LysHis: 1.345 ± 0.04	5.977LysIle: 5.977 ± 0.08	7.281LysLys: 7.281 ± 0.113	6.642LysLeu: 6.642 ± 0.106	2.301LysMet: 2.301 ± 0.049	3.966LysAsn: 3.966 ± 0.082	2.563LysPro: 2.563 ± 0.056	3.217LysGln: 3.217 ± 0.061	3.66LysArg: 3.66 ± 0.067	3.633LysSer: 3.633 ± 0.057	3.979LysThr: 3.979 ± 0.074	5.001LysVal: 5.001 ± 0.083	0.892LysTrp: 0.892 ± 0.036	2.438LysTyr: 2.438 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	7.13LeuAla: 7.13 ± 0.108	0.655LeuCys: 0.655 ± 0.027	4.544LeuAsp: 4.544 ± 0.08	6.948LeuGlu: 6.948 ± 0.098	4.819LeuPhe: 4.819 ± 0.107	6.267LeuGly: 6.267 ± 0.089	1.983LeuHis: 1.983 ± 0.048	7.469LeuIle: 7.469 ± 0.109	7.585LeuLys: 7.585 ± 0.094	10.008LeuLeu: 10.008 ± 0.137	2.389LeuMet: 2.389 ± 0.052	4.287LeuAsn: 4.287 ± 0.073	4.001LeuPro: 4.001 ± 0.061	3.467LeuGln: 3.467 ± 0.07	3.919LeuArg: 3.919 ± 0.068	6.743LeuSer: 6.743 ± 0.091	5.56LeuThr: 5.56 ± 0.089	5.871LeuVal: 5.871 ± 0.088	0.83LeuTrp: 0.83 ± 0.035	3.076LeuTyr: 3.076 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	1.996MetAla: 1.996 ± 0.046	0.159MetCys: 0.159 ± 0.014	1.304MetAsp: 1.304 ± 0.044	1.986MetGlu: 1.986 ± 0.048	1.105MetPhe: 1.105 ± 0.035	1.65MetGly: 1.65 ± 0.047	0.39MetHis: 0.39 ± 0.02	2.208MetIle: 2.208 ± 0.053	2.697MetLys: 2.697 ± 0.05	2.451MetLeu: 2.451 ± 0.053	0.797MetMet: 0.797 ± 0.033	1.467MetAsn: 1.467 ± 0.043	1.01MetPro: 1.01 ± 0.056	0.784MetGln: 0.784 ± 0.031	1.074MetArg: 1.074 ± 0.04	1.471MetSer: 1.471 ± 0.04	1.359MetThr: 1.359 ± 0.038	1.621MetVal: 1.621 ± 0.041	0.178MetTrp: 0.178 ± 0.015	0.725MetTyr: 0.725 ± 0.035	0.0MetXaa: 0.0 ± 0.0
Asn	2.763AsnAla: 2.763 ± 0.055	0.311AsnCys: 0.311 ± 0.017	2.165AsnAsp: 2.165 ± 0.049	3.708AsnGlu: 3.708 ± 0.07	1.734AsnPhe: 1.734 ± 0.041	3.583AsnGly: 3.583 ± 0.073	1.045AsnHis: 1.045 ± 0.038	3.594AsnIle: 3.594 ± 0.062	3.289AsnLys: 3.289 ± 0.062	3.966AsnLeu: 3.966 ± 0.068	1.207AsnMet: 1.207 ± 0.037	2.021AsnAsn: 2.021 ± 0.057	2.252AsnPro: 2.252 ± 0.055	1.741AsnGln: 1.741 ± 0.048	2.115AsnArg: 2.115 ± 0.06	2.281AsnSer: 2.281 ± 0.056	1.971AsnThr: 1.971 ± 0.048	2.936AsnVal: 2.936 ± 0.058	0.516AsnTrp: 0.516 ± 0.023	1.468AsnTyr: 1.468 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	2.344ProAla: 2.344 ± 0.056	0.243ProCys: 0.243 ± 0.017	2.067ProAsp: 2.067 ± 0.05	3.114ProGlu: 3.114 ± 0.058	2.234ProPhe: 2.234 ± 0.06	2.539ProGly: 2.539 ± 0.068	0.81ProHis: 0.81 ± 0.029	2.976ProIle: 2.976 ± 0.057	2.548ProLys: 2.548 ± 0.058	3.613ProLeu: 3.613 ± 0.056	0.777ProMet: 0.777 ± 0.031	1.775ProAsn: 1.775 ± 0.045	1.14ProPro: 1.14 ± 0.034	1.096ProGln: 1.096 ± 0.036	1.187ProArg: 1.187 ± 0.033	2.331ProSer: 2.331 ± 0.046	1.73ProThr: 1.73 ± 0.046	2.944ProVal: 2.944 ± 0.054	0.363ProTrp: 0.363 ± 0.018	1.488ProTyr: 1.488 ± 0.052	0.0ProXaa: 0.0 ± 0.0
Gln	2.454GlnAla: 2.454 ± 0.048	0.203GlnCys: 0.203 ± 0.016	1.419GlnAsp: 1.419 ± 0.037	2.667GlnGlu: 2.667 ± 0.059	1.665GlnPhe: 1.665 ± 0.043	1.997GlnGly: 1.997 ± 0.047	0.722GlnHis: 0.722 ± 0.028	2.739GlnIle: 2.739 ± 0.057	2.943GlnLys: 2.943 ± 0.064	3.758GlnLeu: 3.758 ± 0.072	1.112GlnMet: 1.112 ± 0.043	1.701GlnAsn: 1.701 ± 0.044	1.195GlnPro: 1.195 ± 0.047	1.54GlnGln: 1.54 ± 0.053	1.419GlnArg: 1.419 ± 0.044	2.022GlnSer: 2.022 ± 0.044	1.829GlnThr: 1.829 ± 0.05	2.09GlnVal: 2.09 ± 0.053	0.346GlnTrp: 0.346 ± 0.019	1.312GlnTyr: 1.312 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.546ArgAla: 2.546 ± 0.054	0.28ArgCys: 0.28 ± 0.016	2.046ArgAsp: 2.046 ± 0.046	3.447ArgGlu: 3.447 ± 0.076	2.096ArgPhe: 2.096 ± 0.047	2.417ArgGly: 2.417 ± 0.051	0.852ArgHis: 0.852 ± 0.03	3.438ArgIle: 3.438 ± 0.065	3.817ArgLys: 3.817 ± 0.074	4.163ArgLeu: 4.163 ± 0.083	1.314ArgMet: 1.314 ± 0.038	2.127ArgAsn: 2.127 ± 0.053	1.47ArgPro: 1.47 ± 0.041	1.539ArgGln: 1.539 ± 0.04	2.079ArgArg: 2.079 ± 0.06	2.271ArgSer: 2.271 ± 0.05	2.053ArgThr: 2.053 ± 0.052	2.632ArgVal: 2.632 ± 0.061	0.418ArgTrp: 0.418 ± 0.023	1.579ArgTyr: 1.579 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.637SerAla: 3.637 ± 0.064	0.418SerCys: 0.418 ± 0.021	2.669SerAsp: 2.669 ± 0.053	3.793SerGlu: 3.793 ± 0.076	3.306SerPhe: 3.306 ± 0.064	4.265SerGly: 4.265 ± 0.065	1.184SerHis: 1.184 ± 0.039	5.003SerIle: 5.003 ± 0.081	4.138SerLys: 4.138 ± 0.071	5.976SerLeu: 5.976 ± 0.084	1.555SerMet: 1.555 ± 0.041	2.483SerAsn: 2.483 ± 0.066	2.129SerPro: 2.129 ± 0.053	1.875SerGln: 1.875 ± 0.045	2.262SerArg: 2.262 ± 0.048	3.637SerSer: 3.637 ± 0.073	2.867SerThr: 2.867 ± 0.055	4.001SerVal: 4.001 ± 0.066	0.579SerTrp: 0.579 ± 0.028	2.129SerTyr: 2.129 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	3.694ThrAla: 3.694 ± 0.074	0.339ThrCys: 0.339 ± 0.021	2.455ThrAsp: 2.455 ± 0.05	3.545ThrGlu: 3.545 ± 0.071	2.526ThrPhe: 2.526 ± 0.053	4.136ThrGly: 4.136 ± 0.068	0.919ThrHis: 0.919 ± 0.037	4.389ThrIle: 4.389 ± 0.071	3.428ThrLys: 3.428 ± 0.059	4.732ThrLeu: 4.732 ± 0.076	1.176ThrMet: 1.176 ± 0.037	2.248ThrAsn: 2.248 ± 0.052	2.179ThrPro: 2.179 ± 0.052	1.123ThrGln: 1.123 ± 0.034	1.785ThrArg: 1.785 ± 0.046	2.934ThrSer: 2.934 ± 0.058	2.564ThrThr: 2.564 ± 0.054	4.016ThrVal: 4.016 ± 0.064	0.457ThrTrp: 0.457 ± 0.022	1.735ThrTyr: 1.735 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	4.554ValAla: 4.554 ± 0.081	0.573ValCys: 0.573 ± 0.026	3.373ValAsp: 3.373 ± 0.072	4.576ValGlu: 4.576 ± 0.094	3.239ValPhe: 3.239 ± 0.065	4.277ValGly: 4.277 ± 0.074	1.39ValHis: 1.39 ± 0.036	5.766ValIle: 5.766 ± 0.085	5.239ValLys: 5.239 ± 0.082	6.657ValLeu: 6.657 ± 0.094	1.734ValMet: 1.734 ± 0.045	3.099ValAsn: 3.099 ± 0.055	2.769ValPro: 2.769 ± 0.061	2.189ValGln: 2.189 ± 0.053	2.778ValArg: 2.778 ± 0.055	4.356ValSer: 4.356 ± 0.076	3.571ValThr: 3.571 ± 0.063	4.739ValVal: 4.739 ± 0.08	0.59ValTrp: 0.59 ± 0.025	2.284ValTyr: 2.284 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.58TrpAla: 0.58 ± 0.025	0.087TrpCys: 0.087 ± 0.009	0.472TrpAsp: 0.472 ± 0.026	0.672TrpGlu: 0.672 ± 0.03	0.514TrpPhe: 0.514 ± 0.029	0.64TrpGly: 0.64 ± 0.029	0.199TrpHis: 0.199 ± 0.015	0.889TrpIle: 0.889 ± 0.035	0.889TrpLys: 0.889 ± 0.039	1.104TrpLeu: 1.104 ± 0.037	0.323TrpMet: 0.323 ± 0.021	0.512TrpAsn: 0.512 ± 0.023	0.265TrpPro: 0.265 ± 0.019	0.338TrpGln: 0.338 ± 0.018	0.393TrpArg: 0.393 ± 0.019	0.546TrpSer: 0.546 ± 0.025	0.443TrpThr: 0.443 ± 0.022	0.579TrpVal: 0.579 ± 0.028	0.132TrpTrp: 0.132 ± 0.012	0.35TrpTyr: 0.35 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.934TyrAla: 1.934 ± 0.041	0.322TyrCys: 0.322 ± 0.019	1.856TyrAsp: 1.856 ± 0.048	2.634TyrGlu: 2.634 ± 0.057	1.861TyrPhe: 1.861 ± 0.052	2.502TyrGly: 2.502 ± 0.063	0.841TyrHis: 0.841 ± 0.031	2.463TyrIle: 2.463 ± 0.052	2.313TyrLys: 2.313 ± 0.05	3.477TyrLeu: 3.477 ± 0.074	0.783TyrMet: 0.783 ± 0.03	1.428TyrAsn: 1.428 ± 0.044	1.475TyrPro: 1.475 ± 0.043	1.364TyrGln: 1.364 ± 0.041	1.737TyrArg: 1.737 ± 0.049	2.098TyrSer: 2.098 ± 0.051	1.625TyrThr: 1.625 ± 0.048	2.178TyrVal: 2.178 ± 0.046	0.424TyrTrp: 0.424 ± 0.024	1.417TyrTyr: 1.417 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3271 proteins (916863 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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