Tatumella ptyseos ATCC 33301
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3337 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A085JL87|A0A085JL87_9GAMM Tyrosine recombinase XerC OS=Tatumella ptyseos ATCC 33301 OX=1005995 GN=xerC PE=3 SV=1
TT1 pKa = 7.4 PPVATDD7 pKa = 4.4 DD8 pKa = 3.26 IAYY11 pKa = 9.02 PEE13 pKa = 4.55 KK14 pKa = 9.94 NTPVSGNVLTNDD26 pKa = 3.35 TDD28 pKa = 4.22 KK29 pKa = 11.65 DD30 pKa = 3.81 GDD32 pKa = 4.12 PLAVTQFTVEE42 pKa = 3.98 NQTAKK47 pKa = 10.83 AGATLTLSGVGTLTISSDD65 pKa = 3.06 GAYY68 pKa = 9.39 TFTPVTGYY76 pKa = 8.91 TGPVPSARR84 pKa = 11.84 YY85 pKa = 7.91 TITDD89 pKa = 3.65 GQATATAVLHH99 pKa = 5.75 FTDD102 pKa = 3.87 ITTGSNTSPVAEE114 pKa = 5.33 DD115 pKa = 3.23 DD116 pKa = 4.07 HH117 pKa = 8.67 AVTKK121 pKa = 10.88 ADD123 pKa = 3.52 TAVSGNVLDD132 pKa = 4.7 NDD134 pKa = 3.75 SDD136 pKa = 4.42 KK137 pKa = 11.72 DD138 pKa = 3.8 GDD140 pKa = 4.08 TLTVTGFSVAGQTLKK155 pKa = 11.02 AGEE158 pKa = 4.17 TATLDD163 pKa = 3.6 GKK165 pKa = 10.05 GTLTIGSNGAYY176 pKa = 9.0 TFTPLTGYY184 pKa = 8.11 TGPVPSATYY193 pKa = 9.67 TITDD197 pKa = 3.74 GQATSSATLSFSAINTPPVAIADD220 pKa = 3.81 HH221 pKa = 6.39 AVIDD225 pKa = 3.96 GDD227 pKa = 4.22 TPVSGNVLSNDD238 pKa = 3.18 TDD240 pKa = 4.03 KK241 pKa = 11.75 DD242 pKa = 3.69 GDD244 pKa = 4.2 TLTVTQFTVDD254 pKa = 3.5 GQTVKK259 pKa = 10.82 AGEE262 pKa = 4.04 TATISGVGTLTISSDD277 pKa = 2.9 GTYY280 pKa = 10.32 TFTPVAGYY288 pKa = 8.51 TEE290 pKa = 4.45 TVPTVSYY297 pKa = 10.21 TITDD301 pKa = 3.92 GQATASATLIFSDD314 pKa = 3.48 TRR316 pKa = 11.84 TPPVMAVDD324 pKa = 5.79 DD325 pKa = 3.94 IAHH328 pKa = 6.92 PEE330 pKa = 4.1 KK331 pKa = 10.76 NSPVTGNVLSNDD343 pKa = 3.15 TDD345 pKa = 3.96 KK346 pKa = 11.79 NGGSLTVTRR355 pKa = 11.84 FTVNDD360 pKa = 3.34 QTLTAGEE367 pKa = 4.45 SATIHH372 pKa = 5.51 GVGVLTINANGDD384 pKa = 3.57 YY385 pKa = 10.1 TFSPEE390 pKa = 3.39 IGYY393 pKa = 10.16 AGPVPTATYY402 pKa = 9.4 TVTNGQSTTSATLSFTDD419 pKa = 5.49 IIDD422 pKa = 4.18 NPPDD426 pKa = 3.68 AVDD429 pKa = 3.58 DD430 pKa = 4.04 HH431 pKa = 8.03 AIVQKK436 pKa = 9.79 NTTLSGNALGNDD448 pKa = 3.36 SDD450 pKa = 4.76 KK451 pKa = 11.72 DD452 pKa = 3.58 GDD454 pKa = 4.07 ALTITDD460 pKa = 4.05 FTVNHH465 pKa = 4.99 QTFKK469 pKa = 10.94 AGEE472 pKa = 3.97 TATISGVGTLTFSTSGQYY490 pKa = 10.4 IFTPVKK496 pKa = 10.29 GYY498 pKa = 10.4 VGPVPAATYY507 pKa = 8.27 TVSDD511 pKa = 3.98 GQLTSQATVYY521 pKa = 10.68 FSDD524 pKa = 4.19 IKK526 pKa = 10.23 NTAPTAVDD534 pKa = 3.29 DD535 pKa = 4.53 HH536 pKa = 6.41 ATTVEE541 pKa = 4.0 NTPVSGNVLSNDD553 pKa = 3.25 SDD555 pKa = 3.72 QDD557 pKa = 3.67 GDD559 pKa = 4.25 SLTVTQFTVAGQTLSAGKK577 pKa = 7.93 TATLSGVGTLTIGSDD592 pKa = 3.09 GGYY595 pKa = 8.22 TFTPATGYY603 pKa = 8.19 TGPVPSVNYY612 pKa = 9.66 TITDD616 pKa = 3.79 GQATSSATLSFSAINTPPVANNDD639 pKa = 3.51 TAVAKK644 pKa = 10.61 EE645 pKa = 3.88 NTAVSGNVLSNDD657 pKa = 3.18 TDD659 pKa = 3.99 KK660 pKa = 11.75 DD661 pKa = 3.61 GDD663 pKa = 4.21 TLSVTQFTVDD673 pKa = 3.53 GQTLSAGQTATLSGVGTLTIGSNGDD698 pKa = 3.35 YY699 pKa = 10.46 TFTPVTDD706 pKa = 3.52 YY707 pKa = 10.69 TGPVPSATYY716 pKa = 9.67 TITDD720 pKa = 3.74 GQATSSAMLSFSAINTPPVAEE741 pKa = 4.72 NDD743 pKa = 3.36 TAAPEE748 pKa = 4.35 KK749 pKa = 11.05 NSTTTGNVLTNDD761 pKa = 3.23 SDD763 pKa = 3.99 KK764 pKa = 11.71 DD765 pKa = 3.56 HH766 pKa = 7.42 DD767 pKa = 4.32 TLSVTTFTVNNQTLNAGEE785 pKa = 4.14 TATISGVGTLTIDD798 pKa = 3.58 SNGDD802 pKa = 3.29 YY803 pKa = 10.87 SFTPVTGYY811 pKa = 9.18 TGAVPSAKK819 pKa = 9.19 YY820 pKa = 9.19 TITDD824 pKa = 3.71 GQATATATLSFNNIVNTPPSPQDD847 pKa = 3.2 DD848 pKa = 4.25 TISVQEE854 pKa = 4.08 NTPATGNVLSNDD866 pKa = 3.3 SDD868 pKa = 4.22 KK869 pKa = 11.74 DD870 pKa = 3.61 GDD872 pKa = 4.21 TLTVTQFTIDD882 pKa = 3.64 GQTLKK887 pKa = 10.95 AGEE890 pKa = 4.22 TATLDD895 pKa = 4.22 GIGTLTIGSDD905 pKa = 3.57 GAFTFTPEE913 pKa = 3.54 KK914 pKa = 10.25 GYY916 pKa = 9.19 TGSVPSATYY925 pKa = 9.44 TVTDD929 pKa = 4.04 GQTTASAHH937 pKa = 6.68 LNIQDD942 pKa = 3.22 ITQTYY947 pKa = 8.47 TNTPPVANDD956 pKa = 3.2 DD957 pKa = 3.86 TAVAEE962 pKa = 4.34 EE963 pKa = 4.06 NTAVSGNVLTNDD975 pKa = 3.29 TDD977 pKa = 4.15 KK978 pKa = 11.69 DD979 pKa = 3.82 GDD981 pKa = 4.2 TLTVTQFTVDD991 pKa = 3.77 GQTLTAGQTATINGVGTLTIGSDD1014 pKa = 3.22 GTYY1017 pKa = 10.1 TFTPVTGYY1025 pKa = 9.14 TGAVPSASYY1034 pKa = 10.03 TITDD1038 pKa = 3.86 GQATASATLSFSAINTPPVANNDD1061 pKa = 3.21 TATAKK1066 pKa = 10.67 EE1067 pKa = 3.95 NTAVSGNVLSNDD1079 pKa = 3.18 TDD1081 pKa = 4.03 KK1082 pKa = 11.75 DD1083 pKa = 3.69 GDD1085 pKa = 4.03 TLTVTGFTVDD1095 pKa = 3.64 GQTLTAGEE1103 pKa = 4.3 TATLSGVGTLTIGSDD1118 pKa = 3.35 GAYY1121 pKa = 9.28 TFTPVTGYY1129 pKa = 9.14 TGAVPSATYY1138 pKa = 9.69 TITDD1142 pKa = 3.77 GQATASAVLSFTAINTPPVAVNDD1165 pKa = 3.86 YY1166 pKa = 9.28 VTVDD1170 pKa = 3.17 KK1171 pKa = 11.01 DD1172 pKa = 3.95 SPVSGNVLNNDD1183 pKa = 3.27 TDD1185 pKa = 4.18 KK1186 pKa = 11.72 DD1187 pKa = 3.87 GDD1189 pKa = 4.21 TLSVTQFSVDD1199 pKa = 3.51 GQTLNAGEE1207 pKa = 4.26 TATLSGVGTLTIGSDD1222 pKa = 3.35 GAYY1225 pKa = 9.37 TFTPVSGYY1233 pKa = 10.63 VGAVPAATYY1242 pKa = 8.43 TISDD1246 pKa = 3.86 GQSTAEE1252 pKa = 3.63 ASVIFDD1258 pKa = 3.9 HH1259 pKa = 7.15 APVIEE1264 pKa = 4.34 ITQSTEE1270 pKa = 3.42 VTYY1273 pKa = 10.41 TPGTDD1278 pKa = 2.59 ISGFNSSSWQNNWTSAGWKK1297 pKa = 9.43 GVWPSYY1303 pKa = 10.75 SSTSHH1308 pKa = 6.54 LVNLSADD1315 pKa = 3.5 GGSYY1319 pKa = 9.51 TGTMNAATTFNTNDD1333 pKa = 3.46 VLDD1336 pKa = 4.48 LTVGWNNGWSGTDD1349 pKa = 3.5 DD1350 pKa = 3.67 GSSAVLSFSYY1360 pKa = 8.82 GTQTLFSITTPGQSTTGTTPGAEE1383 pKa = 4.0 YY1384 pKa = 11.18 ADD1386 pKa = 3.83 AQKK1389 pKa = 10.84 YY1390 pKa = 10.38 AYY1392 pKa = 7.64 ITLSKK1397 pKa = 10.39 GIEE1400 pKa = 3.83 VSLDD1404 pKa = 3.35 GGKK1407 pKa = 7.68 TWLDD1411 pKa = 3.24 GSTPIPFEE1419 pKa = 3.72 TWRR1422 pKa = 11.84 LYY1424 pKa = 10.9 QKK1426 pKa = 10.64 DD1427 pKa = 3.64 QTVTDD1432 pKa = 4.0 SDD1434 pKa = 4.02 LSHH1437 pKa = 7.71 ILVKK1441 pKa = 10.69 GMQTGVSDD1449 pKa = 4.2 HH1450 pKa = 7.05 LVLNWDD1456 pKa = 4.13 APGSPADD1463 pKa = 4.16 DD1464 pKa = 3.8 FQISGVSVSQVTEE1477 pKa = 4.13 TTSDD1481 pKa = 3.59 SDD1483 pKa = 4.02 SHH1485 pKa = 6.11 TLTYY1489 pKa = 10.76 DD1490 pKa = 3.41 PSTSSGTDD1498 pKa = 3.17 STSSQLAVLPNISVSDD1514 pKa = 3.67 SDD1516 pKa = 4.14 GDD1518 pKa = 3.97 NDD1520 pKa = 3.73 IQSATITLSGKK1531 pKa = 10.47 SGDD1534 pKa = 3.89 QLWINGMDD1542 pKa = 3.77 ASTHH1546 pKa = 4.75 TAGKK1550 pKa = 10.2 ASYY1553 pKa = 10.57 SLTSDD1558 pKa = 3.78 GEE1560 pKa = 4.51 GTLTLTLKK1568 pKa = 10.81 AGTSGSLSSSDD1579 pKa = 2.95 WNTLLEE1585 pKa = 4.04 GLRR1588 pKa = 11.84 FGTTGDD1594 pKa = 3.06 SGTRR1598 pKa = 11.84 QLTVTVNDD1606 pKa = 4.23 GQLDD1610 pKa = 4.41 SNTEE1614 pKa = 4.03 SVDD1617 pKa = 3.31 INVTGSASSGDD1628 pKa = 3.78 LPGYY1632 pKa = 10.05 SADD1635 pKa = 3.79 TQTTTTSSLLSTYY1648 pKa = 10.14 SLEE1651 pKa = 4.28 NVSDD1655 pKa = 3.96 LASASDD1661 pKa = 3.58 TSSASTTEE1669 pKa = 4.03 TLPTLQSLLSTSSTTTSSTGSSASSTTASSSSATDD1704 pKa = 3.44 SLHH1707 pKa = 5.21 TTSEE1711 pKa = 4.17 TSSALLAKK1719 pKa = 10.22 EE1720 pKa = 5.21 DD1721 pKa = 3.98 EE1722 pKa = 4.54 LSSNNNTVV1730 pKa = 2.58
Molecular weight: 176.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.678
IPC2_protein 3.579
IPC_protein 3.668
Toseland 3.414
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.478
Grimsley 3.312
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.101
Thurlkill 3.478
EMBOSS 3.643
Sillero 3.783
Patrickios 1.189
IPC_peptide 3.668
IPC2_peptide 3.757
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A085JPE5|A0A085JPE5_9GAMM Uncharacterized protein OS=Tatumella ptyseos ATCC 33301 OX=1005995 GN=GTPT_0515 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.22 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LTVSKK46 pKa = 10.99
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3337
0
3337
1003587
37
2922
300.7
33.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.432 ± 0.049
1.115 ± 0.016
5.12 ± 0.035
5.478 ± 0.046
3.816 ± 0.03
7.541 ± 0.039
2.314 ± 0.02
5.838 ± 0.038
3.938 ± 0.031
10.932 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.624 ± 0.023
3.54 ± 0.032
4.646 ± 0.031
4.732 ± 0.043
5.888 ± 0.04
6.477 ± 0.04
5.564 ± 0.038
6.758 ± 0.032
1.411 ± 0.017
2.836 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here