Acidomyces richmondensis BFW

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Teratosphaeriaceae; Acidomyces; unclassified Acidomyces; Acidomyces sp. 'richmondensis'

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11098 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A150VB75|A0A150VB75_9PEZI AP complex subunit sigma OS=Acidomyces richmondensis BFW OX=766039 GN=M433DRAFT_2520 PE=3 SV=1
CC1 pKa = 7.48FLTLPDD7 pKa = 5.21DD8 pKa = 4.33PLSAEE13 pKa = 4.34GLSTPWTLGAPCSQAVTAQQAFAEE37 pKa = 4.44ASVFDD42 pKa = 4.07PTTNTVSVYY51 pKa = 10.37HH52 pKa = 6.4PLVINDD58 pKa = 3.22GMTPQVDD65 pKa = 3.84PVVPDD70 pKa = 3.74LPEE73 pKa = 4.06GAIVGLWFGFNGGVLQLLDD92 pKa = 3.82KK93 pKa = 10.82EE94 pKa = 4.72GRR96 pKa = 11.84DD97 pKa = 3.92TNEE100 pKa = 3.71SPTLQSIDD108 pKa = 4.25CVNGLPGVNGDD119 pKa = 3.71VFGQVSWCNTQPFWAAVNEE138 pKa = 4.29SFAAGKK144 pKa = 9.97IDD146 pKa = 3.84VPEE149 pKa = 5.34LGTDD153 pKa = 3.31HH154 pKa = 7.23NGRR157 pKa = 11.84PCPTSRR163 pKa = 11.84SFEE166 pKa = 4.1IVDD169 pKa = 3.91ACPSDD174 pKa = 4.08NVPTQYY180 pKa = 11.61LLLSDD185 pKa = 4.57GSTVQDD191 pKa = 3.25NASNRR196 pKa = 11.84EE197 pKa = 3.94KK198 pKa = 10.96FPDD201 pKa = 3.51AEE203 pKa = 4.51VINNASDD210 pKa = 3.29EE211 pKa = 4.25SLIANILDD219 pKa = 3.69PAIGCTPFLGEE230 pKa = 4.45NLDD233 pKa = 4.31DD234 pKa = 5.27PGTMFTSLALNEE246 pKa = 4.36LQAKK250 pKa = 9.27AHH252 pKa = 4.83QQAPIALVPLNDD264 pKa = 4.61PDD266 pKa = 4.18TLLTSDD272 pKa = 4.35GQVSPAKK279 pKa = 9.6TNAYY283 pKa = 9.63RR284 pKa = 11.84LGVNQPFLTASGPDD298 pKa = 3.61DD299 pKa = 4.26GSLDD303 pKa = 4.41FYY305 pKa = 10.64CSGMIEE311 pKa = 3.72IAPRR315 pKa = 11.84FFLDD319 pKa = 3.52NQDD322 pKa = 3.53TFTGMTSPATSVGNNLFTFMCNRR345 pKa = 11.84YY346 pKa = 9.68LEE348 pKa = 4.45SLTMLGCPKK357 pKa = 10.51NSSQPVACTLDD368 pKa = 3.66SNGAATSS375 pKa = 3.51

Molecular weight: 39.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A150VDT9|A0A150VDT9_9PEZI DNA polymerase (Fragment) OS=Acidomyces richmondensis BFW OX=766039 GN=M433DRAFT_49008 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.38TRR48 pKa = 11.84IGII51 pKa = 4.0

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11098

0

11098

4519851

49

4874

407.3

45.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.63 ± 0.022

1.359 ± 0.01

5.463 ± 0.018

6.206 ± 0.022

3.679 ± 0.015

6.975 ± 0.024

2.509 ± 0.01

5.047 ± 0.017

4.797 ± 0.02

8.874 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.009

3.702 ± 0.012

5.824 ± 0.025

4.106 ± 0.019

6.436 ± 0.022

7.958 ± 0.029

5.787 ± 0.016

6.04 ± 0.016

1.489 ± 0.009

2.815 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski