Human adenovirus 56
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E1AI11|E1AI11_9ADEN E1B 55 kDa protein OS=Human adenovirus 56 OX=880565 GN=E1B PE=3 SV=1
MM1 pKa = 7.47 RR2 pKa = 11.84 HH3 pKa = 5.98 LRR5 pKa = 11.84 LLPSTVPGEE14 pKa = 4.16 LAVLMLEE21 pKa = 4.64 DD22 pKa = 4.47 FVDD25 pKa = 4.04 TVLEE29 pKa = 4.63 DD30 pKa = 3.85 EE31 pKa = 4.76 LHH33 pKa = 6.57 PSPFEE38 pKa = 4.82 LGPTLQDD45 pKa = 3.63 LYY47 pKa = 11.36 DD48 pKa = 4.71 LEE50 pKa = 5.3 VDD52 pKa = 4.74 AHH54 pKa = 7.26 DD55 pKa = 5.62 DD56 pKa = 3.91 DD57 pKa = 5.34 PNEE60 pKa = 3.98 EE61 pKa = 4.1 AVNLIFPEE69 pKa = 4.24 SMILQADD76 pKa = 4.0 IANEE80 pKa = 4.15 STPLHH85 pKa = 6.33 TPTLSPIPEE94 pKa = 4.36 LEE96 pKa = 4.34 EE97 pKa = 4.11 EE98 pKa = 4.64 DD99 pKa = 4.64 EE100 pKa = 4.71 LDD102 pKa = 3.55 LRR104 pKa = 11.84 CYY106 pKa = 10.79 EE107 pKa = 4.21 EE108 pKa = 5.23 GFPPSDD114 pKa = 3.74 SEE116 pKa = 4.91 DD117 pKa = 3.45 EE118 pKa = 4.43 RR119 pKa = 11.84 GEE121 pKa = 3.79 QTMALISDD129 pKa = 4.34 YY130 pKa = 11.55 ACVTVEE136 pKa = 3.91 EE137 pKa = 4.23 QVVIEE142 pKa = 4.39 NSTEE146 pKa = 3.85 PVEE149 pKa = 4.53 GCRR152 pKa = 11.84 KK153 pKa = 9.06 CQYY156 pKa = 10.51 HH157 pKa = 6.7 RR158 pKa = 11.84 DD159 pKa = 3.58 KK160 pKa = 11.56 SGDD163 pKa = 3.73 PNASCALCYY172 pKa = 9.3 MKK174 pKa = 9.56 QTFSFIYY181 pKa = 10.25 SPVSEE186 pKa = 5.06 DD187 pKa = 3.15 EE188 pKa = 4.37 SSPSEE193 pKa = 4.08 EE194 pKa = 4.56 DD195 pKa = 3.49 HH196 pKa = 7.15 PSPPDD201 pKa = 3.52 LTDD204 pKa = 3.65 DD205 pKa = 3.95 TPLQVHH211 pKa = 6.55 RR212 pKa = 11.84 PTPVRR217 pKa = 11.84 ASGEE221 pKa = 3.57 RR222 pKa = 11.84 RR223 pKa = 11.84 AAVEE227 pKa = 4.11 KK228 pKa = 10.82 IEE230 pKa = 5.54 DD231 pKa = 4.22 LLHH234 pKa = 7.54 DD235 pKa = 4.08 MGGDD239 pKa = 3.59 EE240 pKa = 5.63 PLDD243 pKa = 3.88 LSLKK247 pKa = 10.14 RR248 pKa = 11.84 PRR250 pKa = 11.84 NN251 pKa = 3.53
Molecular weight: 28.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.129
IPC2_protein 4.05
IPC_protein 4.024
Toseland 3.846
ProMoST 4.151
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.872
Rodwell 3.859
Grimsley 3.745
Solomon 3.986
Lehninger 3.935
Nozaki 4.088
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.884
Sillero 4.139
Patrickios 3.249
IPC_peptide 3.986
IPC2_peptide 4.126
IPC2.peptide.svr19 4.047
Protein with the highest isoelectric point:
>tr|E1AI22|E1AI22_9ADEN Isoform of E1AI23 Pre-protein VI OS=Human adenovirus 56 OX=880565 GN=L3 PE=2 SV=1
MM1 pKa = 7.77 ALTCRR6 pKa = 11.84 LRR8 pKa = 11.84 IPVPNYY14 pKa = 8.76 RR15 pKa = 11.84 GRR17 pKa = 11.84 SRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GMAGSGLNRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AMRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 LSGGFLPALIPIIAAAIGTIPGIASVALQASQRR73 pKa = 11.84 RR74 pKa = 3.83
Molecular weight: 8.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.93
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
22
36
11115
49
1176
308.8
34.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.224 ± 0.496
1.979 ± 0.324
5.29 ± 0.272
6.577 ± 0.521
3.842 ± 0.241
6.37 ± 0.289
2.564 ± 0.242
3.797 ± 0.231
3.869 ± 0.386
9.591 ± 0.429
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.798 ± 0.254
4.184 ± 0.467
6.712 ± 0.288
4.283 ± 0.295
7.512 ± 0.659
6.541 ± 0.286
5.722 ± 0.306
6.532 ± 0.23
1.323 ± 0.111
3.293 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here