Desulfonauticus submarinus
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2044 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H0EYP9|A0A1H0EYP9_9DELT Acetoin utilization protein AcuB OS=Desulfonauticus submarinus OX=206665 GN=SAMN04488516_1103 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.2 KK3 pKa = 10.41 YY4 pKa = 10.49 FFLFMFLTIFFSFLINFAYY23 pKa = 10.06 AADD26 pKa = 4.5 YY27 pKa = 10.6 ILQSGQTSDD36 pKa = 3.16 QTNAKK41 pKa = 9.95 DD42 pKa = 3.19 ITGAPNFLNDD52 pKa = 3.61 NDD54 pKa = 4.19 SFTIEE59 pKa = 4.01 SGASIGLDD67 pKa = 2.9 TGIYY71 pKa = 10.88 DD72 pKa = 3.89 MAIAGNNTNTITIRR86 pKa = 11.84 TGGNVIFSYY95 pKa = 11.45 NMLGAATLYY104 pKa = 10.78 GIYY107 pKa = 9.89 VQNNNTINNAGNISSISNSAASTEE131 pKa = 4.04 IYY133 pKa = 10.24 GIHH136 pKa = 6.56 ASDD139 pKa = 4.22 YY140 pKa = 9.17 NTIINSGDD148 pKa = 3.18 ISIMTDD154 pKa = 3.07 TNIGNGEE161 pKa = 4.15 VYY163 pKa = 10.69 GIYY166 pKa = 10.54 ANNYY170 pKa = 6.09 NTISNSDD177 pKa = 4.27 SISVIANSNDD187 pKa = 2.63 NGYY190 pKa = 11.23 AYY192 pKa = 10.2 GIYY195 pKa = 10.72 ANDD198 pKa = 3.83 SNNISNSGSIDD209 pKa = 3.23 ANANNNHH216 pKa = 5.93 NEE218 pKa = 3.12 GRR220 pKa = 11.84 AYY222 pKa = 10.32 GISANASNIITNSGSINATANDD244 pKa = 3.71 NDD246 pKa = 4.08 DD247 pKa = 3.69 GEE249 pKa = 5.68 AYY251 pKa = 10.32 GIYY254 pKa = 10.11 AYY256 pKa = 10.28 DD257 pKa = 3.72 YY258 pKa = 11.38 NNISNSGTIYY268 pKa = 10.87 VIANNNNDD276 pKa = 3.1 GTEE279 pKa = 3.69 AA280 pKa = 3.7
Molecular weight: 29.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.888
IPC2_protein 3.567
IPC_protein 3.579
Toseland 3.35
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.401
Grimsley 3.261
Solomon 3.579
Lehninger 3.528
Nozaki 3.719
DTASelect 3.999
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.706
Patrickios 0.006
IPC_peptide 3.567
IPC2_peptide 3.668
IPC2.peptide.svr19 3.679
Protein with the highest isoelectric point:
>tr|A0A1H0F8Q4|A0A1H0F8Q4_9DELT Cytochrome d ubiquinol oxidase subunit II OS=Desulfonauticus submarinus OX=206665 GN=SAMN04488516_11137 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.17 QPSKK9 pKa = 8.88 IKK11 pKa = 10.27 RR12 pKa = 11.84 KK13 pKa = 9.0 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 5.09 GFLVRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 GGKK28 pKa = 7.75 QVIRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.59 GRR39 pKa = 11.84 KK40 pKa = 8.66 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.199
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.457
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.179
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2044
0
2044
651272
39
2021
318.6
36.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.056 ± 0.054
1.28 ± 0.025
4.5 ± 0.035
6.842 ± 0.062
5.315 ± 0.05
6.29 ± 0.051
1.706 ± 0.021
8.612 ± 0.055
9.393 ± 0.063
11.282 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.893 ± 0.026
4.766 ± 0.041
3.983 ± 0.037
3.464 ± 0.032
3.82 ± 0.041
5.717 ± 0.042
4.382 ± 0.036
6.089 ± 0.056
1.086 ± 0.019
3.525 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here