Gordonia phage Utz
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160DFT8|A0A160DFT8_9CAUD Uncharacterized protein OS=Gordonia phage Utz OX=1838081 GN=23 PE=4 SV=1
MM1 pKa = 7.92 ADD3 pKa = 3.27 TTFSLDD9 pKa = 3.53 EE10 pKa = 4.57 LPTVVFTASFGSGGQPGLPSNTISIRR36 pKa = 11.84 GTMANPHH43 pKa = 6.03 YY44 pKa = 10.89 NPDD47 pKa = 3.27 YY48 pKa = 10.86 DD49 pKa = 4.06 YY50 pKa = 11.71 SGNANGLNASNDD62 pKa = 2.68 PWKK65 pKa = 10.66 RR66 pKa = 11.84 HH67 pKa = 4.59 TEE69 pKa = 4.16 TLVVTVMSFEE79 pKa = 4.57 GPNAPVPADD88 pKa = 4.52 PPPADD93 pKa = 4.58 PDD95 pKa = 3.68 PTPGQEE101 pKa = 4.13 PTDD104 pKa = 3.76 GG105 pKa = 3.69
Molecular weight: 11.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.029
IPC2_protein 3.884
IPC_protein 3.821
Toseland 3.617
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 3.083
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|A0A160DIG2|A0A160DIG2_9CAUD Uncharacterized protein OS=Gordonia phage Utz OX=1838081 GN=57 PE=4 SV=1
MM1 pKa = 6.98 NTTAADD7 pKa = 3.43 QPTTEE12 pKa = 3.98 RR13 pKa = 11.84 SQFAEE18 pKa = 3.48 RR19 pKa = 11.84 DD20 pKa = 3.5 NFRR23 pKa = 11.84 DD24 pKa = 3.38 LTPYY28 pKa = 10.68 EE29 pKa = 3.99 EE30 pKa = 5.72 AILHH34 pKa = 6.09 GMTKK38 pKa = 9.99 PAYY41 pKa = 9.28 FPGRR45 pKa = 11.84 GYY47 pKa = 11.45 FMVQGVYY54 pKa = 9.65 QGYY57 pKa = 10.84 ADD59 pKa = 4.44 DD60 pKa = 4.87 RR61 pKa = 11.84 VITHH65 pKa = 7.31 RR66 pKa = 11.84 DD67 pKa = 3.01 GTIEE71 pKa = 4.09 VVPDD75 pKa = 3.37 PRR77 pKa = 11.84 IARR80 pKa = 11.84 TDD82 pKa = 2.97 RR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 ARR87 pKa = 11.84 NKK89 pKa = 9.81 AGRR92 pKa = 11.84 KK93 pKa = 6.62 SRR95 pKa = 11.84 RR96 pKa = 11.84 INRR99 pKa = 11.84 IRR101 pKa = 11.84 ARR103 pKa = 11.84 RR104 pKa = 3.47
Molecular weight: 12.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.838
IPC_protein 10.965
Toseland 10.818
ProMoST 11.228
Dawson 10.921
Bjellqvist 10.789
Wikipedia 11.272
Rodwell 10.818
Grimsley 10.994
Solomon 11.199
Lehninger 11.125
Nozaki 10.804
DTASelect 10.789
Thurlkill 10.847
EMBOSS 11.272
Sillero 10.877
Patrickios 10.526
IPC_peptide 11.199
IPC2_peptide 10.028
IPC2.peptide.svr19 8.77
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
71
0
71
16136
54
1847
227.3
24.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.626 ± 0.48
0.806 ± 0.138
7.071 ± 0.32
5.385 ± 0.298
2.683 ± 0.159
8.497 ± 0.339
2.058 ± 0.161
4.462 ± 0.15
3.291 ± 0.269
7.623 ± 0.201
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.088 ± 0.205
2.857 ± 0.137
6.018 ± 0.224
3.384 ± 0.126
7.542 ± 0.486
5.392 ± 0.223
7.009 ± 0.264
7.753 ± 0.215
2.15 ± 0.127
2.305 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here