Modestobacter versicolor
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4717 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A323VIT2|A0A323VIT2_9ACTN ATP-dependent DNA ligase OS=Modestobacter versicolor OX=429133 GN=DMO24_02595 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.64 LSTTTRR8 pKa = 11.84 AAAVSASLAAVLALAACGASNEE30 pKa = 4.52 DD31 pKa = 3.64 SGSDD35 pKa = 3.33 SGSGGGSDD43 pKa = 4.13 SAAEE47 pKa = 4.0 LSGSLVGAGASSQQSAMEE65 pKa = 4.09 AWQAGFEE72 pKa = 4.25 GEE74 pKa = 4.28 YY75 pKa = 10.32 PDD77 pKa = 4.36 VQFSYY82 pKa = 11.11 APEE85 pKa = 4.41 GSGAGRR91 pKa = 11.84 EE92 pKa = 3.98 QFINGSADD100 pKa = 3.34 FAGSDD105 pKa = 3.21 AALDD109 pKa = 4.03 EE110 pKa = 4.83 EE111 pKa = 4.79 EE112 pKa = 4.35 LTAASEE118 pKa = 4.26 RR119 pKa = 11.84 CAGGEE124 pKa = 4.12 VFEE127 pKa = 4.74 LPNYY131 pKa = 9.76 ISPIAVAFNLEE142 pKa = 4.38 GVDD145 pKa = 4.06 EE146 pKa = 5.03 LNLTPATIANIFNGTITTWNDD167 pKa = 2.9 PAIAADD173 pKa = 3.83 NADD176 pKa = 3.42 ATLPATAITPVHH188 pKa = 6.42 RR189 pKa = 11.84 ADD191 pKa = 5.34 DD192 pKa = 3.86 SGTTEE197 pKa = 3.92 NFTDD201 pKa = 4.15 YY202 pKa = 10.94 LHH204 pKa = 6.51 QAAADD209 pKa = 3.52 VWTAEE214 pKa = 4.25 PDD216 pKa = 4.15 GVWPLEE222 pKa = 4.37 GGEE225 pKa = 4.36 SAQGTSGVIEE235 pKa = 4.25 VVTNTVGAITYY246 pKa = 10.35 ADD248 pKa = 3.63 ASRR251 pKa = 11.84 AGDD254 pKa = 3.48 LGVANVAVGSDD265 pKa = 4.03 FVAPSPEE272 pKa = 3.37 AAAAVVEE279 pKa = 4.11 NSPAVEE285 pKa = 4.06 GRR287 pKa = 11.84 GEE289 pKa = 3.91 YY290 pKa = 10.87 DD291 pKa = 3.38 FAIEE295 pKa = 4.24 VNRR298 pKa = 11.84 LTEE301 pKa = 4.05 GTGEE305 pKa = 4.2 YY306 pKa = 10.05 PVVLVSYY313 pKa = 10.52 HH314 pKa = 6.26 IGCVEE319 pKa = 4.0 YY320 pKa = 10.69 DD321 pKa = 3.86 DD322 pKa = 5.42 QEE324 pKa = 4.23 KK325 pKa = 10.99 ADD327 pKa = 3.8 NVKK330 pKa = 11.06 AFMNYY335 pKa = 9.44 VISEE339 pKa = 4.37 DD340 pKa = 3.85 GQATAAEE347 pKa = 4.34 NAGNAQISDD356 pKa = 3.95 TLRR359 pKa = 11.84 EE360 pKa = 4.06 QAQGAVDD367 pKa = 5.53 AITAAGG373 pKa = 3.34
Molecular weight: 37.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.427
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.516
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.554
Nozaki 3.719
DTASelect 3.91
Thurlkill 3.465
EMBOSS 3.528
Sillero 3.757
Patrickios 1.151
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.688
Protein with the highest isoelectric point:
>tr|A0A323V9V3|A0A323V9V3_9ACTN Uncharacterized protein OS=Modestobacter versicolor OX=429133 GN=DMO24_13190 PE=4 SV=1
MM1 pKa = 7.56 SILSKK6 pKa = 11.12 LMGTAQQTAGRR17 pKa = 11.84 GRR19 pKa = 11.84 RR20 pKa = 11.84 VGGRR24 pKa = 11.84 PATGRR29 pKa = 11.84 RR30 pKa = 11.84 TTGTGRR36 pKa = 11.84 TAGGTARR43 pKa = 11.84 MARR46 pKa = 11.84 GTTSRR51 pKa = 11.84 GRR53 pKa = 11.84 AGAPAAGGLGKK64 pKa = 10.47 LLSSFAKK71 pKa = 10.16 RR72 pKa = 11.84 RR73 pKa = 3.58
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.676
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.398
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4717
0
4717
1332854
24
1538
282.6
29.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.34 ± 0.05
0.65 ± 0.01
6.233 ± 0.031
5.442 ± 0.034
2.596 ± 0.024
9.663 ± 0.03
2.001 ± 0.02
2.698 ± 0.03
1.353 ± 0.025
10.806 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.578 ± 0.013
1.43 ± 0.016
6.154 ± 0.026
2.921 ± 0.021
7.75 ± 0.039
4.916 ± 0.024
6.197 ± 0.029
10.093 ± 0.04
1.459 ± 0.016
1.72 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here