Levilactobacillus suantsaii
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2163 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q0VJ24|A0A4Q0VJ24_9LACO Uncharacterized protein OS=Levilactobacillus suantsaii OX=2292255 GN=DXH47_08810 PE=4 SV=1
MM1 pKa = 6.99 ITAQVVFATITGNNEE16 pKa = 4.06 DD17 pKa = 3.59 IADD20 pKa = 4.54 IITEE24 pKa = 4.05 KK25 pKa = 11.02 LEE27 pKa = 4.12 SLGCQVTEE35 pKa = 4.39 TEE37 pKa = 4.4 ISQADD42 pKa = 3.37 ATDD45 pKa = 3.7 FQTADD50 pKa = 3.06 ICVVCPYY57 pKa = 10.11 TYY59 pKa = 10.8 DD60 pKa = 3.36 EE61 pKa = 4.91 GALPDD66 pKa = 4.03 EE67 pKa = 4.92 GLDD70 pKa = 4.28 FYY72 pKa = 11.73 EE73 pKa = 5.14 DD74 pKa = 3.75 LQGLDD79 pKa = 4.01 LTGKK83 pKa = 10.13 LYY85 pKa = 10.95 GVAGSGDD92 pKa = 3.63 TFYY95 pKa = 11.39 GQYY98 pKa = 11.22 YY99 pKa = 9.54 NISVDD104 pKa = 3.1 KK105 pKa = 10.32 FAAAFQQAHH114 pKa = 5.85 ATPGAPDD121 pKa = 3.48 VKK123 pKa = 10.78 INLAPDD129 pKa = 3.61 KK130 pKa = 11.21 DD131 pKa = 5.04 DD132 pKa = 4.55 IRR134 pKa = 11.84 QLDD137 pKa = 3.71 AFAQQLVSSHH147 pKa = 5.75 QDD149 pKa = 2.97 QKK151 pKa = 11.97
Molecular weight: 16.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.516
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.872
DTASelect 4.126
Thurlkill 3.579
EMBOSS 3.719
Sillero 3.872
Patrickios 0.693
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A4Q0VID4|A0A4Q0VID4_9LACO DNA repair ATPase OS=Levilactobacillus suantsaii OX=2292255 GN=DXH47_03850 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.59 KK9 pKa = 7.38 RR10 pKa = 11.84 HH11 pKa = 5.81 RR12 pKa = 11.84 SRR14 pKa = 11.84 VHH16 pKa = 6.12 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 HH29 pKa = 3.58 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.39 GRR39 pKa = 11.84 KK40 pKa = 8.52 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2163
0
2163
659888
24
2765
305.1
33.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.514 ± 0.062
0.438 ± 0.011
5.85 ± 0.049
4.31 ± 0.05
3.857 ± 0.041
6.935 ± 0.049
2.545 ± 0.031
5.812 ± 0.052
5.128 ± 0.065
10.234 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.455 ± 0.026
4.03 ± 0.038
4.066 ± 0.039
5.265 ± 0.049
4.646 ± 0.051
5.54 ± 0.103
7.311 ± 0.056
7.444 ± 0.042
1.209 ± 0.023
3.41 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here