Klebsiella phage ST437-OXA245phi4.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Felsduovirus; Klebsiella virus ST437OXA245phi4-1

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482IMP6|A0A482IMP6_9CAUD Tail protein OS=Klebsiella phage ST437-OXA245phi4.1 OX=2510486 PE=4 SV=1
MM1 pKa = 7.42AVIDD5 pKa = 4.87LSQLPPPQIVDD16 pKa = 3.52EE17 pKa = 4.79PDD19 pKa = 3.3FEE21 pKa = 4.61TLLTEE26 pKa = 4.69RR27 pKa = 11.84KK28 pKa = 9.86AEE30 pKa = 4.22FVALYY35 pKa = 9.26PAEE38 pKa = 4.11EE39 pKa = 4.24QEE41 pKa = 4.27AVARR45 pKa = 11.84TLTLEE50 pKa = 3.98SEE52 pKa = 4.93PIVKK56 pKa = 8.7TLQEE60 pKa = 3.77NVYY63 pKa = 10.59RR64 pKa = 11.84EE65 pKa = 3.9LLLRR69 pKa = 11.84QRR71 pKa = 11.84INEE74 pKa = 3.94AARR77 pKa = 11.84AVMVAYY83 pKa = 10.37SGSDD87 pKa = 3.34DD88 pKa = 5.21LDD90 pKa = 3.54NLGANNNVQRR100 pKa = 11.84RR101 pKa = 11.84VITPADD107 pKa = 3.79DD108 pKa = 3.51TTTPPTEE115 pKa = 4.3AEE117 pKa = 4.23MEE119 pKa = 3.94SDD121 pKa = 2.65ADD123 pKa = 3.49YY124 pKa = 10.68RR125 pKa = 11.84QRR127 pKa = 11.84IPAAFEE133 pKa = 4.07GMSVAGPVGAYY144 pKa = 8.33EE145 pKa = 4.06FHH147 pKa = 7.02ALSADD152 pKa = 3.18GRR154 pKa = 11.84VADD157 pKa = 5.22ASAFSPAPAEE167 pKa = 4.16VVVTILARR175 pKa = 11.84DD176 pKa = 3.81GDD178 pKa = 4.25GTAPDD183 pKa = 4.95DD184 pKa = 3.91LLQVVGVALNDD195 pKa = 3.39EE196 pKa = 4.53AVRR199 pKa = 11.84PVADD203 pKa = 3.34RR204 pKa = 11.84VSVRR208 pKa = 11.84SAEE211 pKa = 3.96IVRR214 pKa = 11.84YY215 pKa = 9.67EE216 pKa = 3.88IDD218 pKa = 3.18AVLYY222 pKa = 8.8VYY224 pKa = 9.63PGPAKK229 pKa = 10.45EE230 pKa = 4.92PILAAAKK237 pKa = 9.51EE238 pKa = 4.01QGTAYY243 pKa = 9.86INEE246 pKa = 3.94QRR248 pKa = 11.84RR249 pKa = 11.84LGRR252 pKa = 11.84DD253 pKa = 3.05VRR255 pKa = 11.84LSAIYY260 pKa = 9.96AALHH264 pKa = 4.63VQGVQRR270 pKa = 11.84VEE272 pKa = 4.02LMQPLADD279 pKa = 3.66MVIDD283 pKa = 3.8KK284 pKa = 8.59TQASYY289 pKa = 9.0CTDD292 pKa = 3.96FKK294 pKa = 11.81AEE296 pKa = 3.95IGGSDD301 pKa = 3.47EE302 pKa = 4.06

Molecular weight: 32.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482IM29|A0A482IM29_9CAUD Capsid scaffolding protein OS=Klebsiella phage ST437-OXA245phi4.1 OX=2510486 PE=4 SV=1
MM1 pKa = 7.95AEE3 pKa = 4.03LQEE6 pKa = 3.83VDD8 pKa = 3.12AWLEE12 pKa = 3.72ALLAGLEE19 pKa = 3.9PAARR23 pKa = 11.84KK24 pKa = 10.25RR25 pKa = 11.84MMRR28 pKa = 11.84EE29 pKa = 3.45LAQQLRR35 pKa = 11.84RR36 pKa = 11.84SQQKK40 pKa = 9.63NIRR43 pKa = 11.84MQRR46 pKa = 11.84NPDD49 pKa = 2.92GTAYY53 pKa = 10.1EE54 pKa = 4.13PRR56 pKa = 11.84RR57 pKa = 11.84VTARR61 pKa = 11.84TKK63 pKa = 8.63TGRR66 pKa = 11.84IRR68 pKa = 11.84RR69 pKa = 11.84QMFAKK74 pKa = 10.34LRR76 pKa = 11.84TAKK79 pKa = 10.07YY80 pKa = 9.37LKK82 pKa = 10.42AAASPDD88 pKa = 3.6SASVEE93 pKa = 4.08FEE95 pKa = 3.66GRR97 pKa = 11.84VQRR100 pKa = 11.84IARR103 pKa = 11.84VHH105 pKa = 6.27HH106 pKa = 5.92YY107 pKa = 10.35GLRR110 pKa = 11.84DD111 pKa = 3.17RR112 pKa = 11.84VSRR115 pKa = 11.84RR116 pKa = 11.84GPEE119 pKa = 3.66VQYY122 pKa = 10.0SQRR125 pKa = 11.84RR126 pKa = 11.84LLGINDD132 pKa = 3.72EE133 pKa = 4.51VEE135 pKa = 4.89DD136 pKa = 3.74ITRR139 pKa = 11.84EE140 pKa = 3.94TLLDD144 pKa = 4.71WIQKK148 pKa = 9.41

Molecular weight: 17.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12309

37

876

232.2

26.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.27 ± 0.552

1.105 ± 0.14

5.809 ± 0.249

6.272 ± 0.354

3.558 ± 0.22

6.556 ± 0.38

2.08 ± 0.195

5.565 ± 0.295

5.427 ± 0.324

9.538 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.163

4.29 ± 0.217

4.192 ± 0.236

4.233 ± 0.233

6.442 ± 0.424

6.207 ± 0.226

5.833 ± 0.333

6.207 ± 0.301

1.535 ± 0.141

3.112 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski