Streptococcus satellite phage Javan620
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZX62|A0A4D5ZX62_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan620 OX=2558788 GN=JavanS620_0012 PE=4 SV=1
MM1 pKa = 7.13 YY2 pKa = 10.29 NEE4 pKa = 5.34 EE5 pKa = 4.08 IAKK8 pKa = 9.49 MILSFEE14 pKa = 4.41 KK15 pKa = 10.46 DD16 pKa = 2.98 DD17 pKa = 4.29 CRR19 pKa = 11.84 PEE21 pKa = 4.37 GFDD24 pKa = 3.15 WDD26 pKa = 4.22 DD27 pKa = 4.43 FEE29 pKa = 4.85 VTAKK33 pKa = 10.49 QLIASGQIYY42 pKa = 10.61 ASLKK46 pKa = 9.6 VDD48 pKa = 3.52 YY49 pKa = 10.3 LSPYY53 pKa = 10.53 VDD55 pKa = 5.63 FEE57 pKa = 4.31
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.063
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.719
ProMoST 4.012
Dawson 3.897
Bjellqvist 4.139
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.024
Patrickios 3.083
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|A0A4D5ZUS7|A0A4D5ZUS7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan620 OX=2558788 GN=JavanS620_0007 PE=4 SV=1
MM1 pKa = 7.9 KK2 pKa = 9.36 ITEE5 pKa = 4.1 HH6 pKa = 6.75 KK7 pKa = 10.33 KK8 pKa = 9.62 RR9 pKa = 11.84 DD10 pKa = 3.23 GSTVYY15 pKa = 10.32 RR16 pKa = 11.84 ASIYY20 pKa = 10.8 LGVDD24 pKa = 3.53 KK25 pKa = 10.47 ITGKK29 pKa = 10.69 KK30 pKa = 7.7 VTTKK34 pKa = 9.54 VTGRR38 pKa = 11.84 TKK40 pKa = 10.78 KK41 pKa = 9.78 EE42 pKa = 3.6 LKK44 pKa = 10.82 NNIKK48 pKa = 10.37 LAEE51 pKa = 4.18 SDD53 pKa = 4.05 FKK55 pKa = 11.68 NKK57 pKa = 10.06 GGSRR61 pKa = 11.84 HH62 pKa = 5.28 KK63 pKa = 10.59 AISITKK69 pKa = 9.04 YY70 pKa = 10.78 EE71 pKa = 4.12 EE72 pKa = 4.72 LALLWWDD79 pKa = 3.88 SYY81 pKa = 11.33 KK82 pKa = 9.87 HH83 pKa = 4.51 TVKK86 pKa = 10.63 PNSQDD91 pKa = 3.07 NTRR94 pKa = 11.84 KK95 pKa = 9.94 LLYY98 pKa = 10.52 NHH100 pKa = 7.38 ILPLFGDD107 pKa = 3.92 YY108 pKa = 10.57 KK109 pKa = 10.67 LSKK112 pKa = 10.53 LSTPLIQSIINKK124 pKa = 9.55 LADD127 pKa = 3.69 RR128 pKa = 11.84 TNKK131 pKa = 9.96 GEE133 pKa = 4.0 KK134 pKa = 10.02 GSYY137 pKa = 9.15 LHH139 pKa = 6.58 YY140 pKa = 11.08 DD141 pKa = 4.4 KK142 pKa = 11.26 INALNKK148 pKa = 10.2 RR149 pKa = 11.84 ILQYY153 pKa = 10.84 GVVMQAIPFNPARR166 pKa = 11.84 EE167 pKa = 4.24 VILPRR172 pKa = 11.84 NTQKK176 pKa = 10.83 SKK178 pKa = 9.4 RR179 pKa = 11.84 QKK181 pKa = 9.23 VKK183 pKa = 10.27 HH184 pKa = 5.68 FEE186 pKa = 3.98 NHH188 pKa = 5.49 EE189 pKa = 3.79 LRR191 pKa = 11.84 QFLDD195 pKa = 3.14 YY196 pKa = 10.92 MNNLDD201 pKa = 4.1 LDD203 pKa = 4.33 RR204 pKa = 11.84 YY205 pKa = 9.06 RR206 pKa = 11.84 YY207 pKa = 10.09 SYY209 pKa = 11.27 EE210 pKa = 3.44 LTLYY214 pKa = 10.68 KK215 pKa = 10.39 FLLATGCRR223 pKa = 11.84 INEE226 pKa = 3.75 ALALNWEE233 pKa = 5.27 DD234 pKa = 4.0 IDD236 pKa = 4.51 LDD238 pKa = 3.63 NAIIHH243 pKa = 5.55 ITKK246 pKa = 8.66 TLNYY250 pKa = 8.48 KK251 pKa = 9.4 QEE253 pKa = 4.1 INSPKK258 pKa = 10.43 SKK260 pKa = 10.66 ASYY263 pKa = 10.28 RR264 pKa = 11.84 DD265 pKa = 3.18 ISIDD269 pKa = 3.58 SQTVNMLKK277 pKa = 10.15 AYY279 pKa = 10.44 QRR281 pKa = 11.84 MQIQEE286 pKa = 3.55 AWKK289 pKa = 9.83 LGRR292 pKa = 11.84 SEE294 pKa = 4.74 KK295 pKa = 10.79 VVFSDD300 pKa = 6.4 FIHH303 pKa = 7.08 EE304 pKa = 4.28 YY305 pKa = 9.74 PNNRR309 pKa = 11.84 TLQTRR314 pKa = 11.84 LRR316 pKa = 11.84 THH318 pKa = 7.1 FKK320 pKa = 10.55 NAGVPNIGFHH330 pKa = 6.22 GFRR333 pKa = 11.84 HH334 pKa = 4.87 THH336 pKa = 6.58 ASLLLNSGIPYY347 pKa = 10.01 KK348 pKa = 10.42 EE349 pKa = 3.75 LQTRR353 pKa = 11.84 LGHH356 pKa = 5.26 STISMTMDD364 pKa = 3.2 IYY366 pKa = 11.65 SHH368 pKa = 6.95 LSKK371 pKa = 10.86 EE372 pKa = 4.26 NEE374 pKa = 4.16 KK375 pKa = 10.85 KK376 pKa = 10.42 AVSYY380 pKa = 9.92 FEE382 pKa = 4.51 KK383 pKa = 10.47 AISSII388 pKa = 3.71
Molecular weight: 45.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.068
IPC2_protein 9.297
IPC_protein 9.194
Toseland 9.955
ProMoST 9.619
Dawson 10.16
Bjellqvist 9.809
Wikipedia 10.321
Rodwell 10.657
Grimsley 10.233
Solomon 10.175
Lehninger 10.131
Nozaki 9.926
DTASelect 9.809
Thurlkill 9.999
EMBOSS 10.35
Sillero 10.058
Patrickios 10.116
IPC_peptide 10.175
IPC2_peptide 8.273
IPC2.peptide.svr19 8.268
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
2890
57
413
152.1
17.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.121 ± 0.517
0.588 ± 0.153
6.332 ± 0.574
8.512 ± 0.574
4.291 ± 0.334
3.772 ± 0.387
1.626 ± 0.439
8.408 ± 0.604
9.204 ± 0.553
10.346 ± 0.462
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.491 ± 0.212
6.955 ± 0.55
2.422 ± 0.211
4.118 ± 0.387
4.81 ± 0.412
5.813 ± 0.392
5.952 ± 0.405
3.737 ± 0.22
0.865 ± 0.162
4.637 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here