Desulfoglaeba alkanexedens ALDC
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2850 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8L1K2|A0A4P8L1K2_9DELT MarR family transcriptional regulator OS=Desulfoglaeba alkanexedens ALDC OX=980445 GN=FDQ92_00270 PE=4 SV=1
MM1 pKa = 7.81 DD2 pKa = 5.74 RR3 pKa = 11.84 YY4 pKa = 10.37 VCQICGYY11 pKa = 10.7 VYY13 pKa = 10.83 DD14 pKa = 5.34 PDD16 pKa = 5.05 QGDD19 pKa = 4.12 PDD21 pKa = 3.91 NGIAPGTPFEE31 pKa = 4.86 KK32 pKa = 10.92 LPDD35 pKa = 3.7 DD36 pKa = 3.82 WTCPVCGASKK46 pKa = 11.02 SEE48 pKa = 4.15 FEE50 pKa = 4.7 KK51 pKa = 10.98 EE52 pKa = 3.79
Molecular weight: 5.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.822
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.617
ProMoST 3.948
Dawson 3.821
Bjellqvist 4.075
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.541
Solomon 3.795
Lehninger 3.757
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.694
EMBOSS 3.795
Sillero 3.948
Patrickios 0.401
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.915
Protein with the highest isoelectric point:
>tr|A0A4P8L2D2|A0A4P8L2D2_9DELT ABC_transp_aux domain-containing protein OS=Desulfoglaeba alkanexedens ALDC OX=980445 GN=FDQ92_07635 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.2 RR12 pKa = 11.84 KK13 pKa = 7.55 RR14 pKa = 11.84 THH16 pKa = 5.91 GFLVRR21 pKa = 11.84 MATRR25 pKa = 11.84 SGRR28 pKa = 11.84 AVIRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.59 GRR39 pKa = 11.84 KK40 pKa = 8.66 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2850
0
2850
914423
22
2062
320.9
35.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.424 ± 0.053
1.298 ± 0.022
5.304 ± 0.032
7.289 ± 0.046
4.047 ± 0.029
7.911 ± 0.036
2.249 ± 0.018
5.216 ± 0.036
4.17 ± 0.038
10.504 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.325 ± 0.019
2.567 ± 0.022
5.116 ± 0.029
3.062 ± 0.029
8.013 ± 0.046
5.193 ± 0.031
4.684 ± 0.028
7.771 ± 0.043
1.281 ± 0.019
2.578 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here