Aliishimia ponticola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Aliishimia

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S4NC01|A0A4S4NC01_9RHOB Autoinducer 2-binding protein LsrB OS=Aliishimia ponticola OX=2499833 GN=rhaS PE=3 SV=1
MM1 pKa = 7.13IRR3 pKa = 11.84SAIFMPLLLLPTLVAADD20 pKa = 4.17APQLQNSGPIIYY32 pKa = 10.51LSDD35 pKa = 3.74NLDD38 pKa = 3.64EE39 pKa = 5.74PDD41 pKa = 3.42MLGYY45 pKa = 10.75CIDD48 pKa = 3.76TQDD51 pKa = 3.12RR52 pKa = 11.84GQTEE56 pKa = 4.81RR57 pKa = 11.84IQLHH61 pKa = 4.98SCKK64 pKa = 9.69PDD66 pKa = 3.23TDD68 pKa = 4.1DD69 pKa = 3.3ATGRR73 pKa = 11.84DD74 pKa = 3.64VLFSLNADD82 pKa = 3.53TGRR85 pKa = 11.84IEE87 pKa = 4.02HH88 pKa = 5.9QEE90 pKa = 3.79YY91 pKa = 10.98AGFCLTADD99 pKa = 3.94PDD101 pKa = 3.5AAATVFGLVTCSDD114 pKa = 3.87AEE116 pKa = 4.19DD117 pKa = 3.27QKK119 pKa = 11.16FTFDD123 pKa = 3.48SQSGQIHH130 pKa = 6.7PADD133 pKa = 5.48DD134 pKa = 3.7DD135 pKa = 4.57TMCLTAAAEE144 pKa = 4.29STQAGPFYY152 pKa = 11.0SRR154 pKa = 11.84ALGLDD159 pKa = 2.7ACGAVDD165 pKa = 4.74AALQSFTMQQ174 pKa = 3.12

Molecular weight: 18.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S4NEL1|A0A4S4NEL1_9RHOB 50S ribosomal protein L5 OS=Aliishimia ponticola OX=2499833 GN=rplE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3826

0

3826

1230905

33

4060

321.7

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.211 ± 0.056

0.906 ± 0.012

6.396 ± 0.04

5.79 ± 0.042

3.761 ± 0.025

8.757 ± 0.044

2.051 ± 0.022

5.177 ± 0.028

3.123 ± 0.034

9.895 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.806 ± 0.021

2.623 ± 0.025

5.059 ± 0.03

3.338 ± 0.018

6.427 ± 0.052

5.183 ± 0.028

5.63 ± 0.056

7.241 ± 0.037

1.382 ± 0.02

2.242 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski