Klebsiella phage ST512-KPC3phi13.5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6DBX8|A0A5J6DBX8_9CAUD Uncharacterized protein OS=Klebsiella phage ST512-KPC3phi13.5 OX=2555916 PE=4 SV=1
MM1 pKa = 8.13RR2 pKa = 11.84NGQHH6 pKa = 5.8YY7 pKa = 10.15AYY9 pKa = 8.81PNPSNATPGGMTYY22 pKa = 10.25RR23 pKa = 11.84QYY25 pKa = 11.41LIAKK29 pKa = 7.75IAPVMITNFFSNDD42 pKa = 2.17AWTDD46 pKa = 3.37YY47 pKa = 11.74DD48 pKa = 4.25DD49 pKa = 4.75LARR52 pKa = 11.84TLMMAVDD59 pKa = 5.04AIIEE63 pKa = 4.31AEE65 pKa = 4.11QEE67 pKa = 3.95TAEE70 pKa = 4.09

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6DB44|A0A5J6DB44_9CAUD Uncharacterized protein OS=Klebsiella phage ST512-KPC3phi13.5 OX=2555916 PE=4 SV=1
MM1 pKa = 7.7IDD3 pKa = 3.41NRR5 pKa = 11.84TVSAIDD11 pKa = 3.79LALQKK16 pKa = 10.72HH17 pKa = 5.26PTPVGDD23 pKa = 4.01LFAAIRR29 pKa = 11.84HH30 pKa = 5.09GRR32 pKa = 11.84MKK34 pKa = 10.47RR35 pKa = 11.84CFSRR39 pKa = 11.84DD40 pKa = 2.83TAIRR44 pKa = 11.84YY45 pKa = 7.74LAFFMTSRR53 pKa = 11.84AFGRR57 pKa = 11.84SGFKK61 pKa = 9.87QRR63 pKa = 11.84YY64 pKa = 7.55PDD66 pKa = 3.42VQVIHH71 pKa = 6.88PLNPEE76 pKa = 3.9LSSWQRR82 pKa = 11.84GAVTTEE88 pKa = 3.68YY89 pKa = 10.8FNAHH93 pKa = 4.66QRR95 pKa = 11.84TVRR98 pKa = 11.84RR99 pKa = 11.84LRR101 pKa = 11.84RR102 pKa = 11.84ILARR106 pKa = 11.84KK107 pKa = 9.79RR108 pKa = 11.84EE109 pKa = 3.96MQKK112 pKa = 9.07WCKK115 pKa = 10.08KK116 pKa = 6.96WDD118 pKa = 3.66AMHH121 pKa = 7.06DD122 pKa = 3.58RR123 pKa = 11.84YY124 pKa = 11.06VKK126 pKa = 10.27EE127 pKa = 4.09RR128 pKa = 11.84EE129 pKa = 3.79EE130 pKa = 4.02LQACKK135 pKa = 10.13PGGLSRR141 pKa = 4.61

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

7693

32

525

178.9

19.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.255 ± 0.548

1.235 ± 0.2

5.888 ± 0.31

6.031 ± 0.391

3.562 ± 0.212

7.422 ± 0.307

1.677 ± 0.181

6.226 ± 0.266

5.706 ± 0.349

8.319 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.184

4.212 ± 0.254

4.485 ± 0.287

3.9 ± 0.235

6.265 ± 0.406

6.083 ± 0.256

5.914 ± 0.284

6.421 ± 0.28

1.599 ± 0.176

3.133 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski