Amino acid dipepetide frequency for Enterobacter phage ATCEA85

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.21AlaAla: 11.21 ± 1.165
0.857AlaCys: 0.857 ± 0.334
7.783AlaAsp: 7.783 ± 0.774
5.998AlaGlu: 5.998 ± 0.777
2.356AlaPhe: 2.356 ± 0.417
8.283AlaGly: 8.283 ± 0.619
1.428AlaHis: 1.428 ± 0.311
6.069AlaIle: 6.069 ± 0.799
5.141AlaLys: 5.141 ± 0.993
6.712AlaLeu: 6.712 ± 0.786
3.642AlaMet: 3.642 ± 0.517
5.998AlaAsn: 5.998 ± 0.825
3.285AlaPro: 3.285 ± 0.595
4.213AlaGln: 4.213 ± 0.942
3.856AlaArg: 3.856 ± 0.511
5.284AlaSer: 5.284 ± 0.505
6.712AlaThr: 6.712 ± 0.95
7.712AlaVal: 7.712 ± 0.777
1.214AlaTrp: 1.214 ± 0.264
2.571AlaTyr: 2.571 ± 0.484
0.0AlaXaa: 0.0 ± 0.0
Cys
1.0CysAla: 1.0 ± 0.241
0.071CysCys: 0.071 ± 0.074
1.071CysAsp: 1.071 ± 0.383
0.286CysGlu: 0.286 ± 0.14
0.214CysPhe: 0.214 ± 0.114
1.285CysGly: 1.285 ± 0.456
0.0CysHis: 0.0 ± 0.0
0.357CysIle: 0.357 ± 0.182
0.643CysLys: 0.643 ± 0.298
0.571CysLeu: 0.571 ± 0.276
0.071CysMet: 0.071 ± 0.086
0.357CysAsn: 0.357 ± 0.166
0.428CysPro: 0.428 ± 0.193
0.428CysGln: 0.428 ± 0.199
0.928CysArg: 0.928 ± 0.235
0.857CysSer: 0.857 ± 0.426
0.357CysThr: 0.357 ± 0.163
0.571CysVal: 0.571 ± 0.193
0.143CysTrp: 0.143 ± 0.095
0.428CysTyr: 0.428 ± 0.142
0.0CysXaa: 0.0 ± 0.0
Asp
7.712AspAla: 7.712 ± 0.699
0.643AspCys: 0.643 ± 0.192
4.713AspAsp: 4.713 ± 0.753
4.284AspGlu: 4.284 ± 0.8
3.07AspPhe: 3.07 ± 0.414
5.712AspGly: 5.712 ± 1.076
0.928AspHis: 0.928 ± 0.268
3.356AspIle: 3.356 ± 0.453
2.356AspLys: 2.356 ± 0.301
5.641AspLeu: 5.641 ± 0.603
2.071AspMet: 2.071 ± 0.292
2.428AspAsn: 2.428 ± 0.456
2.356AspPro: 2.356 ± 0.44
2.356AspGln: 2.356 ± 0.442
3.213AspArg: 3.213 ± 0.622
3.784AspSer: 3.784 ± 0.575
2.928AspThr: 2.928 ± 0.477
3.713AspVal: 3.713 ± 0.354
0.785AspTrp: 0.785 ± 0.251
2.142AspTyr: 2.142 ± 0.463
0.0AspXaa: 0.0 ± 0.0
Glu
6.569GluAla: 6.569 ± 0.777
0.785GluCys: 0.785 ± 0.329
3.499GluAsp: 3.499 ± 0.593
3.356GluGlu: 3.356 ± 0.738
2.071GluPhe: 2.071 ± 0.413
3.999GluGly: 3.999 ± 0.555
0.928GluHis: 0.928 ± 0.272
3.784GluIle: 3.784 ± 0.645
3.213GluLys: 3.213 ± 0.419
5.784GluLeu: 5.784 ± 0.863
1.142GluMet: 1.142 ± 0.251
3.213GluAsn: 3.213 ± 0.389
2.071GluPro: 2.071 ± 0.519
3.784GluGln: 3.784 ± 0.633
3.499GluArg: 3.499 ± 0.457
1.499GluSer: 1.499 ± 0.359
2.856GluThr: 2.856 ± 0.473
3.356GluVal: 3.356 ± 0.351
0.785GluTrp: 0.785 ± 0.214
2.285GluTyr: 2.285 ± 0.33
0.0GluXaa: 0.0 ± 0.0
Phe
2.571PheAla: 2.571 ± 0.545
0.5PheCys: 0.5 ± 0.2
2.356PheAsp: 2.356 ± 0.391
1.785PheGlu: 1.785 ± 0.354
1.285PhePhe: 1.285 ± 0.406
2.785PheGly: 2.785 ± 0.386
0.571PheHis: 0.571 ± 0.156
1.999PheIle: 1.999 ± 0.369
2.642PheLys: 2.642 ± 0.549
2.856PheLeu: 2.856 ± 0.468
0.571PheMet: 0.571 ± 0.209
2.499PheAsn: 2.499 ± 0.419
1.0PhePro: 1.0 ± 0.299
1.142PheGln: 1.142 ± 0.219
2.428PheArg: 2.428 ± 0.555
2.213PheSer: 2.213 ± 0.427
2.356PheThr: 2.356 ± 0.376
2.499PheVal: 2.499 ± 0.39
0.214PheTrp: 0.214 ± 0.159
1.0PheTyr: 1.0 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
6.712GlyAla: 6.712 ± 0.692
0.785GlyCys: 0.785 ± 0.263
4.356GlyAsp: 4.356 ± 0.607
5.284GlyGlu: 5.284 ± 0.593
3.07GlyPhe: 3.07 ± 0.374
6.783GlyGly: 6.783 ± 0.893
0.857GlyHis: 0.857 ± 0.294
5.212GlyIle: 5.212 ± 0.538
5.141GlyLys: 5.141 ± 0.609
4.784GlyLeu: 4.784 ± 0.65
2.571GlyMet: 2.571 ± 0.391
4.427GlyAsn: 4.427 ± 0.669
1.714GlyPro: 1.714 ± 0.263
3.999GlyGln: 3.999 ± 0.696
4.784GlyArg: 4.784 ± 0.504
4.356GlySer: 4.356 ± 0.617
4.784GlyThr: 4.784 ± 0.816
5.355GlyVal: 5.355 ± 0.746
1.0GlyTrp: 1.0 ± 0.42
2.856GlyTyr: 2.856 ± 0.656
0.0GlyXaa: 0.0 ± 0.0
His
1.357HisAla: 1.357 ± 0.341
0.357HisCys: 0.357 ± 0.168
0.5HisAsp: 0.5 ± 0.232
1.071HisGlu: 1.071 ± 0.465
0.643HisPhe: 0.643 ± 0.226
0.857HisGly: 0.857 ± 0.252
0.428HisHis: 0.428 ± 0.172
0.785HisIle: 0.785 ± 0.301
0.5HisLys: 0.5 ± 0.194
1.357HisLeu: 1.357 ± 0.359
0.214HisMet: 0.214 ± 0.123
0.714HisAsn: 0.714 ± 0.256
0.714HisPro: 0.714 ± 0.319
0.5HisGln: 0.5 ± 0.187
1.285HisArg: 1.285 ± 0.351
0.714HisSer: 0.714 ± 0.193
0.643HisThr: 0.643 ± 0.201
1.785HisVal: 1.785 ± 0.342
0.214HisTrp: 0.214 ± 0.133
0.5HisTyr: 0.5 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
5.212IleAla: 5.212 ± 0.58
1.0IleCys: 1.0 ± 0.349
4.356IleAsp: 4.356 ± 0.673
3.642IleGlu: 3.642 ± 0.466
1.642IlePhe: 1.642 ± 0.425
3.927IleGly: 3.927 ± 0.442
0.5IleHis: 0.5 ± 0.21
2.713IleIle: 2.713 ± 0.578
2.642IleLys: 2.642 ± 0.359
3.285IleLeu: 3.285 ± 0.558
1.428IleMet: 1.428 ± 0.346
3.499IleAsn: 3.499 ± 0.491
3.427IlePro: 3.427 ± 0.501
3.356IleGln: 3.356 ± 0.438
3.57IleArg: 3.57 ± 0.59
3.427IleSer: 3.427 ± 0.372
4.998IleThr: 4.998 ± 0.715
3.499IleVal: 3.499 ± 0.488
0.5IleTrp: 0.5 ± 0.213
1.214IleTyr: 1.214 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
6.426LysAla: 6.426 ± 0.836
0.5LysCys: 0.5 ± 0.235
3.356LysAsp: 3.356 ± 0.492
2.713LysGlu: 2.713 ± 0.617
1.285LysPhe: 1.285 ± 0.254
4.498LysGly: 4.498 ± 0.56
1.499LysHis: 1.499 ± 0.447
2.428LysIle: 2.428 ± 0.34
3.213LysLys: 3.213 ± 0.482
3.142LysLeu: 3.142 ± 0.403
1.785LysMet: 1.785 ± 0.537
2.356LysAsn: 2.356 ± 0.521
3.142LysPro: 3.142 ± 0.682
2.785LysGln: 2.785 ± 0.503
3.713LysArg: 3.713 ± 0.527
2.785LysSer: 2.785 ± 0.392
3.57LysThr: 3.57 ± 0.5
2.928LysVal: 2.928 ± 0.44
0.643LysTrp: 0.643 ± 0.155
1.856LysTyr: 1.856 ± 0.273
0.0LysXaa: 0.0 ± 0.0
Leu
8.354LeuAla: 8.354 ± 1.123
0.571LeuCys: 0.571 ± 0.221
4.784LeuAsp: 4.784 ± 0.691
3.642LeuGlu: 3.642 ± 0.386
2.285LeuPhe: 2.285 ± 0.398
4.141LeuGly: 4.141 ± 0.546
1.142LeuHis: 1.142 ± 0.362
4.427LeuIle: 4.427 ± 0.576
4.784LeuLys: 4.784 ± 0.719
4.713LeuLeu: 4.713 ± 0.81
1.999LeuMet: 1.999 ± 0.474
4.07LeuAsn: 4.07 ± 0.375
2.642LeuPro: 2.642 ± 0.474
2.642LeuGln: 2.642 ± 0.347
4.07LeuArg: 4.07 ± 0.539
4.07LeuSer: 4.07 ± 0.495
4.713LeuThr: 4.713 ± 0.557
5.07LeuVal: 5.07 ± 0.552
1.642LeuTrp: 1.642 ± 0.476
2.428LeuTyr: 2.428 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
2.285MetAla: 2.285 ± 0.343
0.214MetCys: 0.214 ± 0.138
1.428MetAsp: 1.428 ± 0.382
0.928MetGlu: 0.928 ± 0.302
1.142MetPhe: 1.142 ± 0.331
1.999MetGly: 1.999 ± 0.395
0.143MetHis: 0.143 ± 0.106
2.499MetIle: 2.499 ± 0.432
2.356MetLys: 2.356 ± 0.426
1.999MetLeu: 1.999 ± 0.327
1.0MetMet: 1.0 ± 0.289
1.856MetAsn: 1.856 ± 0.432
1.142MetPro: 1.142 ± 0.268
1.071MetGln: 1.071 ± 0.236
1.642MetArg: 1.642 ± 0.314
2.285MetSer: 2.285 ± 0.452
1.571MetThr: 1.571 ± 0.395
1.428MetVal: 1.428 ± 0.269
0.357MetTrp: 0.357 ± 0.158
1.142MetTyr: 1.142 ± 0.295
0.0MetXaa: 0.0 ± 0.0
Asn
5.284AsnAla: 5.284 ± 0.734
0.714AsnCys: 0.714 ± 0.256
3.07AsnAsp: 3.07 ± 0.509
3.213AsnGlu: 3.213 ± 0.504
1.714AsnPhe: 1.714 ± 0.295
5.498AsnGly: 5.498 ± 0.468
0.928AsnHis: 0.928 ± 0.261
2.642AsnIle: 2.642 ± 0.415
3.213AsnLys: 3.213 ± 0.328
2.428AsnLeu: 2.428 ± 0.437
1.428AsnMet: 1.428 ± 0.288
2.285AsnAsn: 2.285 ± 0.438
2.928AsnPro: 2.928 ± 0.509
1.785AsnGln: 1.785 ± 0.336
2.785AsnArg: 2.785 ± 0.545
2.928AsnSer: 2.928 ± 0.526
2.928AsnThr: 2.928 ± 0.373
2.928AsnVal: 2.928 ± 0.365
0.571AsnTrp: 0.571 ± 0.188
1.999AsnTyr: 1.999 ± 0.429
0.0AsnXaa: 0.0 ± 0.0
Pro
5.07ProAla: 5.07 ± 0.717
0.286ProCys: 0.286 ± 0.153
3.213ProAsp: 3.213 ± 0.512
3.142ProGlu: 3.142 ± 0.66
1.714ProPhe: 1.714 ± 0.409
3.285ProGly: 3.285 ± 0.62
0.643ProHis: 0.643 ± 0.273
2.285ProIle: 2.285 ± 0.339
1.499ProLys: 1.499 ± 0.32
1.571ProLeu: 1.571 ± 0.423
0.714ProMet: 0.714 ± 0.235
1.642ProAsn: 1.642 ± 0.517
1.357ProPro: 1.357 ± 0.341
1.285ProGln: 1.285 ± 0.293
1.142ProArg: 1.142 ± 0.3
1.928ProSer: 1.928 ± 0.396
3.07ProThr: 3.07 ± 0.616
2.928ProVal: 2.928 ± 0.643
1.357ProTrp: 1.357 ± 0.361
1.571ProTyr: 1.571 ± 0.416
0.0ProXaa: 0.0 ± 0.0
Gln
5.855GlnAla: 5.855 ± 0.981
0.214GlnCys: 0.214 ± 0.139
2.071GlnAsp: 2.071 ± 0.363
1.928GlnGlu: 1.928 ± 0.499
2.142GlnPhe: 2.142 ± 0.445
3.713GlnGly: 3.713 ± 0.802
0.571GlnHis: 0.571 ± 0.239
3.427GlnIle: 3.427 ± 0.622
2.071GlnLys: 2.071 ± 0.376
4.855GlnLeu: 4.855 ± 0.626
1.642GlnMet: 1.642 ± 0.28
1.785GlnAsn: 1.785 ± 0.318
1.357GlnPro: 1.357 ± 0.466
3.427GlnGln: 3.427 ± 0.46
2.571GlnArg: 2.571 ± 0.426
1.999GlnSer: 1.999 ± 0.372
2.499GlnThr: 2.499 ± 0.641
1.928GlnVal: 1.928 ± 0.366
1.071GlnTrp: 1.071 ± 0.281
1.428GlnTyr: 1.428 ± 0.3
0.0GlnXaa: 0.0 ± 0.0
Arg
4.356ArgAla: 4.356 ± 0.556
0.714ArgCys: 0.714 ± 0.278
3.356ArgAsp: 3.356 ± 0.535
3.356ArgGlu: 3.356 ± 0.767
1.856ArgPhe: 1.856 ± 0.388
3.285ArgGly: 3.285 ± 0.472
0.928ArgHis: 0.928 ± 0.277
3.784ArgIle: 3.784 ± 0.458
3.07ArgLys: 3.07 ± 0.585
5.212ArgLeu: 5.212 ± 0.602
1.642ArgMet: 1.642 ± 0.342
2.356ArgAsn: 2.356 ± 0.456
1.999ArgPro: 1.999 ± 0.504
3.07ArgGln: 3.07 ± 0.487
3.499ArgArg: 3.499 ± 0.581
2.571ArgSer: 2.571 ± 0.452
2.499ArgThr: 2.499 ± 0.466
4.427ArgVal: 4.427 ± 0.986
1.0ArgTrp: 1.0 ± 0.282
2.213ArgTyr: 2.213 ± 0.405
0.0ArgXaa: 0.0 ± 0.0
Ser
5.641SerAla: 5.641 ± 0.939
0.643SerCys: 0.643 ± 0.221
3.142SerAsp: 3.142 ± 0.46
3.142SerGlu: 3.142 ± 0.419
2.356SerPhe: 2.356 ± 0.461
4.427SerGly: 4.427 ± 0.468
1.357SerHis: 1.357 ± 0.415
3.285SerIle: 3.285 ± 0.63
2.642SerLys: 2.642 ± 0.478
4.07SerLeu: 4.07 ± 0.444
1.714SerMet: 1.714 ± 0.299
2.285SerAsn: 2.285 ± 0.436
2.428SerPro: 2.428 ± 0.39
2.571SerGln: 2.571 ± 0.602
2.499SerArg: 2.499 ± 0.436
2.571SerSer: 2.571 ± 0.366
3.213SerThr: 3.213 ± 0.468
3.856SerVal: 3.856 ± 0.478
0.785SerTrp: 0.785 ± 0.234
1.856SerTyr: 1.856 ± 0.427
0.0SerXaa: 0.0 ± 0.0
Thr
5.926ThrAla: 5.926 ± 0.932
0.5ThrCys: 0.5 ± 0.302
4.07ThrAsp: 4.07 ± 0.738
3.142ThrGlu: 3.142 ± 0.559
2.285ThrPhe: 2.285 ± 0.434
5.212ThrGly: 5.212 ± 1.076
0.928ThrHis: 0.928 ± 0.22
3.213ThrIle: 3.213 ± 0.474
2.428ThrLys: 2.428 ± 0.379
5.284ThrLeu: 5.284 ± 0.58
1.499ThrMet: 1.499 ± 0.252
2.928ThrAsn: 2.928 ± 0.829
3.427ThrPro: 3.427 ± 0.534
2.856ThrGln: 2.856 ± 0.641
2.856ThrArg: 2.856 ± 0.46
3.856ThrSer: 3.856 ± 0.798
5.07ThrThr: 5.07 ± 0.776
5.284ThrVal: 5.284 ± 0.693
1.142ThrTrp: 1.142 ± 0.221
1.856ThrTyr: 1.856 ± 0.37
0.0ThrXaa: 0.0 ± 0.0
Val
5.07ValAla: 5.07 ± 0.592
0.286ValCys: 0.286 ± 0.121
3.999ValAsp: 3.999 ± 0.589
5.284ValGlu: 5.284 ± 0.43
2.571ValPhe: 2.571 ± 0.547
4.998ValGly: 4.998 ± 0.81
0.785ValHis: 0.785 ± 0.359
3.213ValIle: 3.213 ± 0.615
4.141ValLys: 4.141 ± 0.632
3.999ValLeu: 3.999 ± 0.583
1.999ValMet: 1.999 ± 0.517
3.713ValAsn: 3.713 ± 0.495
1.928ValPro: 1.928 ± 0.305
2.642ValGln: 2.642 ± 0.492
4.07ValArg: 4.07 ± 0.459
4.855ValSer: 4.855 ± 0.828
5.427ValThr: 5.427 ± 1.317
3.499ValVal: 3.499 ± 0.464
0.643ValTrp: 0.643 ± 0.241
2.356ValTyr: 2.356 ± 0.47
0.0ValXaa: 0.0 ± 0.0
Trp
1.071TrpAla: 1.071 ± 0.29
0.214TrpCys: 0.214 ± 0.173
0.643TrpAsp: 0.643 ± 0.208
0.571TrpGlu: 0.571 ± 0.211
0.857TrpPhe: 0.857 ± 0.232
1.214TrpGly: 1.214 ± 0.293
0.357TrpHis: 0.357 ± 0.125
0.785TrpIle: 0.785 ± 0.359
0.5TrpLys: 0.5 ± 0.183
1.357TrpLeu: 1.357 ± 0.325
0.286TrpMet: 0.286 ± 0.155
0.571TrpAsn: 0.571 ± 0.275
0.643TrpPro: 0.643 ± 0.284
1.0TrpGln: 1.0 ± 0.276
1.357TrpArg: 1.357 ± 0.276
0.785TrpSer: 0.785 ± 0.261
1.071TrpThr: 1.071 ± 0.299
1.071TrpVal: 1.071 ± 0.265
0.357TrpTrp: 0.357 ± 0.177
0.357TrpTyr: 0.357 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.642TyrAla: 2.642 ± 0.49
0.214TyrCys: 0.214 ± 0.158
2.428TyrAsp: 2.428 ± 0.538
1.714TyrGlu: 1.714 ± 0.236
0.785TyrPhe: 0.785 ± 0.312
2.928TyrGly: 2.928 ± 0.382
0.214TyrHis: 0.214 ± 0.131
1.571TyrIle: 1.571 ± 0.315
2.428TyrLys: 2.428 ± 0.337
2.571TyrLeu: 2.571 ± 0.469
1.0TyrMet: 1.0 ± 0.216
2.285TyrAsn: 2.285 ± 0.456
1.714TyrPro: 1.714 ± 0.378
1.642TyrGln: 1.642 ± 0.288
1.428TyrArg: 1.428 ± 0.309
1.714TyrSer: 1.714 ± 0.311
2.499TyrThr: 2.499 ± 0.459
1.642TyrVal: 1.642 ± 0.406
0.643TyrTrp: 0.643 ± 0.221
1.0TyrTyr: 1.0 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (14006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski