Amino acid dipepetide frequency for Clostridium magnum DSM 2767

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.022AlaAla: 5.022 ± 0.068
0.785AlaCys: 0.785 ± 0.022
2.951AlaAsp: 2.951 ± 0.046
3.957AlaGlu: 3.957 ± 0.046
2.611AlaPhe: 2.611 ± 0.046
4.178AlaGly: 4.178 ± 0.063
0.877AlaHis: 0.877 ± 0.026
5.796AlaIle: 5.796 ± 0.062
4.828AlaLys: 4.828 ± 0.065
6.053AlaLeu: 6.053 ± 0.072
1.797AlaMet: 1.797 ± 0.036
2.788AlaAsn: 2.788 ± 0.045
1.614AlaPro: 1.614 ± 0.041
1.667AlaGln: 1.667 ± 0.037
1.988AlaArg: 1.988 ± 0.04
3.836AlaSer: 3.836 ± 0.061
2.938AlaThr: 2.938 ± 0.043
4.887AlaVal: 4.887 ± 0.058
0.391AlaTrp: 0.391 ± 0.015
2.162AlaTyr: 2.162 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
0.693CysAla: 0.693 ± 0.021
0.235CysCys: 0.235 ± 0.012
0.686CysAsp: 0.686 ± 0.024
0.805CysGlu: 0.805 ± 0.024
0.526CysPhe: 0.526 ± 0.018
1.255CysGly: 1.255 ± 0.032
0.255CysHis: 0.255 ± 0.013
1.192CysIle: 1.192 ± 0.026
1.025CysLys: 1.025 ± 0.023
0.934CysLeu: 0.934 ± 0.024
0.312CysMet: 0.312 ± 0.013
0.715CysAsn: 0.715 ± 0.02
0.559CysPro: 0.559 ± 0.023
0.268CysGln: 0.268 ± 0.013
0.479CysArg: 0.479 ± 0.017
0.866CysSer: 0.866 ± 0.024
0.65CysThr: 0.65 ± 0.02
0.743CysVal: 0.743 ± 0.022
0.077CysTrp: 0.077 ± 0.006
0.47CysTyr: 0.47 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
2.894AspAla: 2.894 ± 0.044
0.649AspCys: 0.649 ± 0.022
2.462AspAsp: 2.462 ± 0.04
4.207AspGlu: 4.207 ± 0.059
2.581AspPhe: 2.581 ± 0.038
3.35AspGly: 3.35 ± 0.067
0.577AspHis: 0.577 ± 0.018
6.059AspIle: 6.059 ± 0.06
5.161AspLys: 5.161 ± 0.059
4.736AspLeu: 4.736 ± 0.048
1.501AspMet: 1.501 ± 0.032
3.118AspAsn: 3.118 ± 0.05
1.438AspPro: 1.438 ± 0.033
1.013AspGln: 1.013 ± 0.025
1.917AspArg: 1.917 ± 0.038
3.329AspSer: 3.329 ± 0.055
2.779AspThr: 2.779 ± 0.048
3.577AspVal: 3.577 ± 0.044
0.385AspTrp: 0.385 ± 0.016
2.404AspTyr: 2.404 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
4.349GluAla: 4.349 ± 0.058
0.769GluCys: 0.769 ± 0.023
4.134GluAsp: 4.134 ± 0.059
6.458GluGlu: 6.458 ± 0.078
2.757GluPhe: 2.757 ± 0.041
4.092GluGly: 4.092 ± 0.053
1.003GluHis: 1.003 ± 0.024
6.492GluIle: 6.492 ± 0.074
7.283GluLys: 7.283 ± 0.071
6.818GluLeu: 6.818 ± 0.068
1.834GluMet: 1.834 ± 0.033
4.885GluAsn: 4.885 ± 0.053
1.406GluPro: 1.406 ± 0.028
2.117GluGln: 2.117 ± 0.046
2.553GluArg: 2.553 ± 0.042
3.603GluSer: 3.603 ± 0.046
3.206GluThr: 3.206 ± 0.045
4.757GluVal: 4.757 ± 0.054
0.442GluTrp: 0.442 ± 0.016
2.839GluTyr: 2.839 ± 0.044
0.0GluXaa: 0.0 ± 0.0
Phe
2.354PheAla: 2.354 ± 0.044
0.582PheCys: 0.582 ± 0.018
2.359PheAsp: 2.359 ± 0.037
2.531PheGlu: 2.531 ± 0.039
1.771PhePhe: 1.771 ± 0.037
2.8PheGly: 2.8 ± 0.047
0.598PheHis: 0.598 ± 0.019
4.322PheIle: 4.322 ± 0.061
3.507PheLys: 3.507 ± 0.045
3.694PheLeu: 3.694 ± 0.054
1.139PheMet: 1.139 ± 0.028
2.723PheAsn: 2.723 ± 0.041
1.221PhePro: 1.221 ± 0.028
1.007PheGln: 1.007 ± 0.022
1.27PheArg: 1.27 ± 0.028
3.188PheSer: 3.188 ± 0.052
2.376PheThr: 2.376 ± 0.038
2.677PheVal: 2.677 ± 0.04
0.322PheTrp: 0.322 ± 0.012
1.689PheTyr: 1.689 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
4.263GlyAla: 4.263 ± 0.066
1.0GlyCys: 1.0 ± 0.029
3.247GlyAsp: 3.247 ± 0.047
4.159GlyGlu: 4.159 ± 0.054
3.098GlyPhe: 3.098 ± 0.048
4.489GlyGly: 4.489 ± 0.061
1.027GlyHis: 1.027 ± 0.023
6.765GlyIle: 6.765 ± 0.069
5.655GlyLys: 5.655 ± 0.06
5.39GlyLeu: 5.39 ± 0.064
1.886GlyMet: 1.886 ± 0.036
3.28GlyAsn: 3.28 ± 0.049
1.273GlyPro: 1.273 ± 0.029
1.66GlyGln: 1.66 ± 0.031
2.288GlyArg: 2.288 ± 0.04
3.914GlySer: 3.914 ± 0.059
3.792GlyThr: 3.792 ± 0.085
4.75GlyVal: 4.75 ± 0.06
0.543GlyTrp: 0.543 ± 0.018
2.949GlyTyr: 2.949 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
0.743HisAla: 0.743 ± 0.023
0.257HisCys: 0.257 ± 0.014
0.68HisAsp: 0.68 ± 0.021
0.862HisGlu: 0.862 ± 0.021
0.634HisPhe: 0.634 ± 0.019
1.018HisGly: 1.018 ± 0.026
0.304HisHis: 0.304 ± 0.015
1.359HisIle: 1.359 ± 0.03
1.101HisLys: 1.101 ± 0.026
1.212HisLeu: 1.212 ± 0.028
0.404HisMet: 0.404 ± 0.015
0.806HisAsn: 0.806 ± 0.024
0.658HisPro: 0.658 ± 0.02
0.382HisGln: 0.382 ± 0.016
0.564HisArg: 0.564 ± 0.017
0.897HisSer: 0.897 ± 0.026
0.742HisThr: 0.742 ± 0.021
0.824HisVal: 0.824 ± 0.019
0.13HisTrp: 0.13 ± 0.01
0.548HisTyr: 0.548 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.995IleAla: 5.995 ± 0.053
1.221IleCys: 1.221 ± 0.026
5.62IleAsp: 5.62 ± 0.057
6.814IleGlu: 6.814 ± 0.072
3.888IlePhe: 3.888 ± 0.06
6.231IleGly: 6.231 ± 0.069
1.313IleHis: 1.313 ± 0.03
8.641IleIle: 8.641 ± 0.093
8.222IleLys: 8.222 ± 0.086
8.516IleLeu: 8.516 ± 0.082
2.31IleMet: 2.31 ± 0.041
5.783IleAsn: 5.783 ± 0.062
3.352IlePro: 3.352 ± 0.044
2.354IleGln: 2.354 ± 0.041
3.169IleArg: 3.169 ± 0.049
6.854IleSer: 6.854 ± 0.07
5.012IleThr: 5.012 ± 0.051
6.236IleVal: 6.236 ± 0.055
0.56IleTrp: 0.56 ± 0.019
3.294IleTyr: 3.294 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.102LysAla: 5.102 ± 0.062
0.903LysCys: 0.903 ± 0.026
5.57LysAsp: 5.57 ± 0.057
7.879LysGlu: 7.879 ± 0.074
3.114LysPhe: 3.114 ± 0.044
5.135LysGly: 5.135 ± 0.064
1.185LysHis: 1.185 ± 0.028
7.942LysIle: 7.942 ± 0.076
7.572LysLys: 7.572 ± 0.075
7.511LysLeu: 7.511 ± 0.067
2.43LysMet: 2.43 ± 0.04
5.9LysAsn: 5.9 ± 0.066
2.169LysPro: 2.169 ± 0.04
2.361LysGln: 2.361 ± 0.041
2.917LysArg: 2.917 ± 0.038
5.079LysSer: 5.079 ± 0.06
4.078LysThr: 4.078 ± 0.053
6.173LysVal: 6.173 ± 0.063
0.64LysTrp: 0.64 ± 0.018
3.767LysTyr: 3.767 ± 0.049
0.0LysXaa: 0.0 ± 0.0
Leu
5.473LeuAla: 5.473 ± 0.067
1.141LeuCys: 1.141 ± 0.027
4.828LeuAsp: 4.828 ± 0.056
6.134LeuGlu: 6.134 ± 0.065
3.506LeuPhe: 3.506 ± 0.048
6.024LeuGly: 6.024 ± 0.068
1.145LeuHis: 1.145 ± 0.025
7.804LeuIle: 7.804 ± 0.078
8.47LeuLys: 8.47 ± 0.064
7.751LeuLeu: 7.751 ± 0.077
2.323LeuMet: 2.323 ± 0.036
5.488LeuAsn: 5.488 ± 0.054
2.882LeuPro: 2.882 ± 0.042
2.453LeuGln: 2.453 ± 0.042
3.186LeuArg: 3.186 ± 0.046
6.755LeuSer: 6.755 ± 0.069
4.588LeuThr: 4.588 ± 0.049
5.5LeuVal: 5.5 ± 0.061
0.611LeuTrp: 0.611 ± 0.021
3.105LeuTyr: 3.105 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
1.849MetAla: 1.849 ± 0.037
0.299MetCys: 0.299 ± 0.013
1.584MetAsp: 1.584 ± 0.031
1.959MetGlu: 1.959 ± 0.033
1.041MetPhe: 1.041 ± 0.029
1.82MetGly: 1.82 ± 0.036
0.359MetHis: 0.359 ± 0.016
2.195MetIle: 2.195 ± 0.039
2.397MetLys: 2.397 ± 0.033
2.341MetLeu: 2.341 ± 0.038
0.709MetMet: 0.709 ± 0.024
1.683MetAsn: 1.683 ± 0.035
0.962MetPro: 0.962 ± 0.026
0.694MetGln: 0.694 ± 0.02
0.881MetArg: 0.881 ± 0.022
1.779MetSer: 1.779 ± 0.034
1.28MetThr: 1.28 ± 0.027
1.716MetVal: 1.716 ± 0.03
0.159MetTrp: 0.159 ± 0.009
0.85MetTyr: 0.85 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.09AsnAla: 3.09 ± 0.045
0.771AsnCys: 0.771 ± 0.024
2.694AsnAsp: 2.694 ± 0.044
3.828AsnGlu: 3.828 ± 0.054
2.429AsnPhe: 2.429 ± 0.035
3.514AsnGly: 3.514 ± 0.063
0.801AsnHis: 0.801 ± 0.023
6.497AsnIle: 6.497 ± 0.075
5.524AsnLys: 5.524 ± 0.056
5.48AsnLeu: 5.48 ± 0.068
1.612AsnMet: 1.612 ± 0.034
3.966AsnAsn: 3.966 ± 0.056
2.127AsnPro: 2.127 ± 0.04
1.422AsnGln: 1.422 ± 0.027
2.03AsnArg: 2.03 ± 0.034
4.144AsnSer: 4.144 ± 0.059
2.976AsnThr: 2.976 ± 0.042
3.699AsnVal: 3.699 ± 0.047
0.487AsnTrp: 0.487 ± 0.017
2.502AsnTyr: 2.502 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
1.684ProAla: 1.684 ± 0.037
0.369ProCys: 0.369 ± 0.016
1.575ProAsp: 1.575 ± 0.03
2.346ProGlu: 2.346 ± 0.036
1.313ProPhe: 1.313 ± 0.027
1.871ProGly: 1.871 ± 0.037
0.506ProHis: 0.506 ± 0.016
2.787ProIle: 2.787 ± 0.046
2.205ProLys: 2.205 ± 0.036
2.536ProLeu: 2.536 ± 0.033
0.775ProMet: 0.775 ± 0.024
1.532ProAsn: 1.532 ± 0.032
0.709ProPro: 0.709 ± 0.02
0.885ProGln: 0.885 ± 0.025
0.908ProArg: 0.908 ± 0.026
1.812ProSer: 1.812 ± 0.029
1.598ProThr: 1.598 ± 0.035
2.289ProVal: 2.289 ± 0.04
0.242ProTrp: 0.242 ± 0.013
1.296ProTyr: 1.296 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
1.592GlnAla: 1.592 ± 0.035
0.322GlnCys: 0.322 ± 0.014
1.293GlnAsp: 1.293 ± 0.029
1.704GlnGlu: 1.704 ± 0.034
1.039GlnPhe: 1.039 ± 0.024
1.687GlnGly: 1.687 ± 0.034
0.39GlnHis: 0.39 ± 0.014
2.33GlnIle: 2.33 ± 0.037
2.199GlnLys: 2.199 ± 0.035
2.41GlnLeu: 2.41 ± 0.039
0.773GlnMet: 0.773 ± 0.023
1.555GlnAsn: 1.555 ± 0.033
0.68GlnPro: 0.68 ± 0.022
0.907GlnGln: 0.907 ± 0.028
1.019GlnArg: 1.019 ± 0.023
1.66GlnSer: 1.66 ± 0.031
1.269GlnThr: 1.269 ± 0.03
1.726GlnVal: 1.726 ± 0.037
0.258GlnTrp: 0.258 ± 0.013
1.099GlnTyr: 1.099 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
1.937ArgAla: 1.937 ± 0.036
0.445ArgCys: 0.445 ± 0.016
1.88ArgAsp: 1.88 ± 0.033
2.86ArgGlu: 2.86 ± 0.046
1.521ArgPhe: 1.521 ± 0.03
2.082ArgGly: 2.082 ± 0.034
0.505ArgHis: 0.505 ± 0.018
3.146ArgIle: 3.146 ± 0.049
3.115ArgLys: 3.115 ± 0.046
2.993ArgLeu: 2.993 ± 0.044
1.009ArgMet: 1.009 ± 0.025
1.986ArgAsn: 1.986 ± 0.035
0.933ArgPro: 0.933 ± 0.026
1.002ArgGln: 1.002 ± 0.025
1.501ArgArg: 1.501 ± 0.033
1.789ArgSer: 1.789 ± 0.032
1.683ArgThr: 1.683 ± 0.03
2.23ArgVal: 2.23 ± 0.04
0.268ArgTrp: 0.268 ± 0.012
1.441ArgTyr: 1.441 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
3.67SerAla: 3.67 ± 0.057
0.795SerCys: 0.795 ± 0.023
3.432SerAsp: 3.432 ± 0.061
4.286SerGlu: 4.286 ± 0.053
3.014SerPhe: 3.014 ± 0.045
4.568SerGly: 4.568 ± 0.061
0.963SerHis: 0.963 ± 0.025
6.642SerIle: 6.642 ± 0.07
5.688SerLys: 5.688 ± 0.064
5.875SerLeu: 5.875 ± 0.059
1.731SerMet: 1.731 ± 0.032
3.752SerAsn: 3.752 ± 0.055
1.791SerPro: 1.791 ± 0.036
1.715SerGln: 1.715 ± 0.031
2.195SerArg: 2.195 ± 0.04
4.728SerSer: 4.728 ± 0.08
3.463SerThr: 3.463 ± 0.056
4.191SerVal: 4.191 ± 0.057
0.464SerTrp: 0.464 ± 0.016
2.534SerTyr: 2.534 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
3.567ThrAla: 3.567 ± 0.058
0.585ThrCys: 0.585 ± 0.021
2.611ThrAsp: 2.611 ± 0.049
3.235ThrGlu: 3.235 ± 0.049
2.192ThrPhe: 2.192 ± 0.036
3.903ThrGly: 3.903 ± 0.05
0.744ThrHis: 0.744 ± 0.023
4.828ThrIle: 4.828 ± 0.054
3.781ThrLys: 3.781 ± 0.044
4.852ThrLeu: 4.852 ± 0.054
1.199ThrMet: 1.199 ± 0.03
2.774ThrAsn: 2.774 ± 0.046
1.946ThrPro: 1.946 ± 0.042
1.194ThrGln: 1.194 ± 0.028
1.593ThrArg: 1.593 ± 0.033
3.357ThrSer: 3.357 ± 0.054
2.88ThrThr: 2.88 ± 0.055
3.856ThrVal: 3.856 ± 0.114
0.408ThrTrp: 0.408 ± 0.017
1.923ThrTyr: 1.923 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
4.398ValAla: 4.398 ± 0.066
0.929ValCys: 0.929 ± 0.023
3.871ValAsp: 3.871 ± 0.055
4.634ValGlu: 4.634 ± 0.06
2.849ValPhe: 2.849 ± 0.04
4.389ValGly: 4.389 ± 0.062
0.909ValHis: 0.909 ± 0.023
6.212ValIle: 6.212 ± 0.063
5.687ValLys: 5.687 ± 0.064
6.002ValLeu: 6.002 ± 0.058
1.664ValMet: 1.664 ± 0.029
3.796ValAsn: 3.796 ± 0.063
2.11ValPro: 2.11 ± 0.043
1.718ValGln: 1.718 ± 0.033
2.158ValArg: 2.158 ± 0.033
4.69ValSer: 4.69 ± 0.06
3.758ValThr: 3.758 ± 0.073
4.908ValVal: 4.908 ± 0.065
0.424ValTrp: 0.424 ± 0.017
2.402ValTyr: 2.402 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
0.411TrpAla: 0.411 ± 0.015
0.113TrpCys: 0.113 ± 0.007
0.399TrpAsp: 0.399 ± 0.017
0.453TrpGlu: 0.453 ± 0.015
0.33TrpPhe: 0.33 ± 0.014
0.5TrpGly: 0.5 ± 0.019
0.122TrpHis: 0.122 ± 0.009
0.67TrpIle: 0.67 ± 0.02
0.538TrpLys: 0.538 ± 0.017
0.642TrpLeu: 0.642 ± 0.022
0.188TrpMet: 0.188 ± 0.011
0.501TrpAsn: 0.501 ± 0.018
0.173TrpPro: 0.173 ± 0.009
0.249TrpGln: 0.249 ± 0.012
0.267TrpArg: 0.267 ± 0.013
0.485TrpSer: 0.485 ± 0.019
0.338TrpThr: 0.338 ± 0.014
0.431TrpVal: 0.431 ± 0.016
0.074TrpTrp: 0.074 ± 0.007
0.29TrpTyr: 0.29 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.04TyrAla: 2.04 ± 0.036
0.577TyrCys: 0.577 ± 0.018
2.26TyrAsp: 2.26 ± 0.043
2.684TyrGlu: 2.684 ± 0.041
1.918TyrPhe: 1.918 ± 0.034
2.521TyrGly: 2.521 ± 0.037
0.541TyrHis: 0.541 ± 0.017
3.704TyrIle: 3.704 ± 0.056
3.473TyrLys: 3.473 ± 0.046
3.422TyrLeu: 3.422 ± 0.058
0.962TyrMet: 0.962 ± 0.023
2.562TyrAsn: 2.562 ± 0.044
1.233TyrPro: 1.233 ± 0.025
0.843TyrGln: 0.843 ± 0.023
1.441TyrArg: 1.441 ± 0.028
2.733TyrSer: 2.733 ± 0.042
2.022TyrThr: 2.022 ± 0.058
2.339TyrVal: 2.339 ± 0.036
0.287TyrTrp: 0.287 ± 0.014
1.744TyrTyr: 1.744 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6083 proteins (1781211 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski