Elephant endotheliotropic herpesvirus 1A
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 120 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S1NI02|A0A0S1NI02_ELHV1 Protein E22A OS=Elephant endotheliotropic herpesvirus 1A OX=759753 GN=E22A PE=4 SV=1
MM1 pKa = 7.61 ARR3 pKa = 11.84 TLYY6 pKa = 10.71 LVLGLATLFCVTEE19 pKa = 4.39 SYY21 pKa = 10.82 PVTNEE26 pKa = 3.82 DD27 pKa = 3.54 TDD29 pKa = 4.4 DD30 pKa = 4.91 LEE32 pKa = 4.4 TTYY35 pKa = 11.13 TSVEE39 pKa = 4.3 TNISSSSDD47 pKa = 2.89 KK48 pKa = 10.75 HH49 pKa = 6.22 GKK51 pKa = 10.34 LNVTCLAKK59 pKa = 10.61 ALPPASNVTWIGLSNIDD76 pKa = 3.66 NSTSITNNTDD86 pKa = 2.4 GTLTVRR92 pKa = 11.84 STLHH96 pKa = 5.92 IADD99 pKa = 4.65 EE100 pKa = 4.25 KK101 pKa = 10.44 RR102 pKa = 11.84 QVNGSAYY109 pKa = 10.22 CRR111 pKa = 11.84 VSHH114 pKa = 5.46 QVSVTYY120 pKa = 10.43 LAAPWNSEE128 pKa = 3.5 DD129 pKa = 4.57 DD130 pKa = 3.45 MGIFGLIMEE139 pKa = 5.14 EE140 pKa = 4.35 LDD142 pKa = 4.63 LSDD145 pKa = 4.56 SYY147 pKa = 12.0 EE148 pKa = 4.06 LVFNDD153 pKa = 4.35 GDD155 pKa = 4.47 DD156 pKa = 3.86 GGEE159 pKa = 3.97 DD160 pKa = 5.01 DD161 pKa = 6.07 EE162 pKa = 6.83 YY163 pKa = 11.69 DD164 pKa = 4.77 DD165 pKa = 5.97 DD166 pKa = 6.81 DD167 pKa = 4.41 EE168 pKa = 7.2 GEE170 pKa = 4.63 DD171 pKa = 4.02 YY172 pKa = 11.5 EE173 pKa = 4.61 EE174 pKa = 5.15 DD175 pKa = 3.53 KK176 pKa = 11.58
Molecular weight: 19.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.439
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 1.1
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.769
Protein with the highest isoelectric point:
>tr|M1RFX5|M1RFX5_ELHV1 ORF-C OS=Elephant endotheliotropic herpesvirus 1A OX=759753 GN=E34 PE=4 SV=1
MM1 pKa = 7.1 YY2 pKa = 10.79 LLVRR6 pKa = 11.84 LFGAGAMIMVVPVSKK21 pKa = 10.7 QDD23 pKa = 3.26 VVDD26 pKa = 4.19 PSRR29 pKa = 11.84 RR30 pKa = 11.84 SSDD33 pKa = 3.09 TLLNRR38 pKa = 11.84 LFSIPSVRR46 pKa = 11.84 STSPKK51 pKa = 9.45 PSHH54 pKa = 6.8 ILQNLLFVSCKK65 pKa = 10.29 SSLKK69 pKa = 10.23 KK70 pKa = 10.01 IYY72 pKa = 10.28 KK73 pKa = 8.38 ITSKK77 pKa = 10.82 CEE79 pKa = 3.74 RR80 pKa = 11.84 KK81 pKa = 10.22 KK82 pKa = 10.96 LDD84 pKa = 3.65 SDD86 pKa = 4.19 HH87 pKa = 6.36 STQHH91 pKa = 7.04 LICTHH96 pKa = 5.97 CTGQPRR102 pKa = 11.84 CLMSSLKK109 pKa = 10.29 RR110 pKa = 11.84 YY111 pKa = 9.87 VRR113 pKa = 11.84 VKK115 pKa = 10.44 CKK117 pKa = 10.24 KK118 pKa = 9.82
Molecular weight: 13.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.232
IPC2_protein 9.428
IPC_protein 9.443
Toseland 10.409
ProMoST 9.984
Dawson 10.511
Bjellqvist 10.145
Wikipedia 10.628
Rodwell 11.096
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.438
DTASelect 10.116
Thurlkill 10.409
EMBOSS 10.789
Sillero 10.438
Patrickios 10.847
IPC_peptide 10.555
IPC2_peptide 9.121
IPC2.peptide.svr19 8.335
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
120
0
120
49069
66
2237
408.9
46.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.03 ± 0.156
2.688 ± 0.147
5.376 ± 0.171
4.718 ± 0.133
4.742 ± 0.192
4.763 ± 0.221
2.761 ± 0.088
6.544 ± 0.227
4.905 ± 0.174
9.731 ± 0.291
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.519 ± 0.093
5.348 ± 0.174
4.675 ± 0.226
3.395 ± 0.234
5.054 ± 0.219
8.38 ± 0.31
7.373 ± 0.238
6.845 ± 0.151
0.927 ± 0.057
4.225 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here