Amino acid dipepetide frequency for Desulfopila aestuarii DSM 18488

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.538AlaAla: 8.538 ± 0.102	1.073AlaCys: 1.073 ± 0.028	4.472AlaAsp: 4.472 ± 0.053	5.951AlaGlu: 5.951 ± 0.072	3.138AlaPhe: 3.138 ± 0.048	6.994AlaGly: 6.994 ± 0.074	1.549AlaHis: 1.549 ± 0.027	6.074AlaIle: 6.074 ± 0.066	4.226AlaLys: 4.226 ± 0.063	8.363AlaLeu: 8.363 ± 0.09	2.723AlaMet: 2.723 ± 0.039	2.821AlaAsn: 2.821 ± 0.045	2.758AlaPro: 2.758 ± 0.047	2.612AlaGln: 2.612 ± 0.043	4.464AlaArg: 4.464 ± 0.062	4.6AlaSer: 4.6 ± 0.06	4.694AlaThr: 4.694 ± 0.066	6.128AlaVal: 6.128 ± 0.066	0.808AlaTrp: 0.808 ± 0.024	2.109AlaTyr: 2.109 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	0.939CysAla: 0.939 ± 0.026	0.289CysCys: 0.289 ± 0.015	0.626CysAsp: 0.626 ± 0.022	0.642CysGlu: 0.642 ± 0.02	0.499CysPhe: 0.499 ± 0.017	1.292CysGly: 1.292 ± 0.032	0.424CysHis: 0.424 ± 0.023	0.755CysIle: 0.755 ± 0.021	0.487CysLys: 0.487 ± 0.015	1.302CysLeu: 1.302 ± 0.031	0.326CysMet: 0.326 ± 0.015	0.486CysAsn: 0.486 ± 0.014	0.748CysPro: 0.748 ± 0.025	0.452CysGln: 0.452 ± 0.016	0.869CysArg: 0.869 ± 0.021	0.888CysSer: 0.888 ± 0.021	0.696CysThr: 0.696 ± 0.019	0.765CysVal: 0.765 ± 0.026	0.152CysTrp: 0.152 ± 0.01	0.386CysTyr: 0.386 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.744AspAla: 3.744 ± 0.051	0.674AspCys: 0.674 ± 0.02	2.797AspAsp: 2.797 ± 0.049	3.644AspGlu: 3.644 ± 0.047	2.4AspPhe: 2.4 ± 0.034	3.94AspGly: 3.94 ± 0.057	1.113AspHis: 1.113 ± 0.026	4.167AspIle: 4.167 ± 0.055	2.556AspLys: 2.556 ± 0.038	5.531AspLeu: 5.531 ± 0.062	1.394AspMet: 1.394 ± 0.032	2.053AspAsn: 2.053 ± 0.036	2.502AspPro: 2.502 ± 0.042	1.889AspGln: 1.889 ± 0.033	2.898AspArg: 2.898 ± 0.042	3.007AspSer: 3.007 ± 0.047	2.727AspThr: 2.727 ± 0.043	3.361AspVal: 3.361 ± 0.043	0.653AspTrp: 0.653 ± 0.023	1.746AspTyr: 1.746 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	5.34GluAla: 5.34 ± 0.055	0.609GluCys: 0.609 ± 0.021	3.02GluAsp: 3.02 ± 0.044	4.91GluGlu: 4.91 ± 0.068	2.181GluPhe: 2.181 ± 0.039	3.927GluGly: 3.927 ± 0.056	1.392GluHis: 1.392 ± 0.032	4.803GluIle: 4.803 ± 0.064	4.902GluLys: 4.902 ± 0.06	6.941GluLeu: 6.941 ± 0.072	2.146GluMet: 2.146 ± 0.039	2.84GluAsn: 2.84 ± 0.043	2.235GluPro: 2.235 ± 0.041	2.953GluGln: 2.953 ± 0.042	3.803GluArg: 3.803 ± 0.052	3.4GluSer: 3.4 ± 0.044	3.404GluThr: 3.404 ± 0.046	4.386GluVal: 4.386 ± 0.052	0.624GluTrp: 0.624 ± 0.022	1.844GluTyr: 1.844 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.389PheAla: 3.389 ± 0.048	0.647PheCys: 0.647 ± 0.022	2.383PheAsp: 2.383 ± 0.034	2.133PheGlu: 2.133 ± 0.041	2.171PhePhe: 2.171 ± 0.045	3.364PheGly: 3.364 ± 0.056	0.901PheHis: 0.901 ± 0.024	2.645PheIle: 2.645 ± 0.045	1.618PheLys: 1.618 ± 0.033	4.222PheLeu: 4.222 ± 0.063	1.038PheMet: 1.038 ± 0.028	1.584PheAsn: 1.584 ± 0.03	1.746PhePro: 1.746 ± 0.037	1.41PheGln: 1.41 ± 0.032	2.029PheArg: 2.029 ± 0.033	3.234PheSer: 3.234 ± 0.048	2.523PheThr: 2.523 ± 0.035	2.711PheVal: 2.711 ± 0.04	0.493PheTrp: 0.493 ± 0.016	1.228PheTyr: 1.228 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	5.688GlyAla: 5.688 ± 0.073	1.254GlyCys: 1.254 ± 0.033	3.602GlyAsp: 3.602 ± 0.05	4.536GlyGlu: 4.536 ± 0.061	3.262GlyPhe: 3.262 ± 0.047	5.654GlyGly: 5.654 ± 0.093	1.553GlyHis: 1.553 ± 0.028	5.456GlyIle: 5.456 ± 0.06	4.621GlyLys: 4.621 ± 0.05	7.33GlyLeu: 7.33 ± 0.076	2.35GlyMet: 2.35 ± 0.038	2.694GlyAsn: 2.694 ± 0.043	2.254GlyPro: 2.254 ± 0.04	2.508GlyGln: 2.508 ± 0.041	4.073GlyArg: 4.073 ± 0.05	4.492GlySer: 4.492 ± 0.063	4.159GlyThr: 4.159 ± 0.051	5.408GlyVal: 5.408 ± 0.064	0.927GlyTrp: 0.927 ± 0.026	2.791GlyTyr: 2.791 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	1.484HisAla: 1.484 ± 0.029	0.345HisCys: 0.345 ± 0.015	1.251HisAsp: 1.251 ± 0.027	1.271HisGlu: 1.271 ± 0.032	0.987HisPhe: 0.987 ± 0.025	1.692HisGly: 1.692 ± 0.037	0.626HisHis: 0.626 ± 0.021	1.359HisIle: 1.359 ± 0.027	0.858HisLys: 0.858 ± 0.024	2.304HisLeu: 2.304 ± 0.04	0.48HisMet: 0.48 ± 0.016	0.788HisAsn: 0.788 ± 0.022	1.221HisPro: 1.221 ± 0.028	0.85HisGln: 0.85 ± 0.021	1.116HisArg: 1.116 ± 0.026	1.327HisSer: 1.327 ± 0.029	1.032HisThr: 1.032 ± 0.026	1.232HisVal: 1.232 ± 0.027	0.239HisTrp: 0.239 ± 0.012	0.725HisTyr: 0.725 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.03IleAla: 6.03 ± 0.053	0.972IleCys: 0.972 ± 0.022	4.065IleAsp: 4.065 ± 0.055	4.401IleGlu: 4.401 ± 0.055	2.935IlePhe: 2.935 ± 0.042	5.222IleGly: 5.222 ± 0.062	1.485IleHis: 1.485 ± 0.028	4.483IleIle: 4.483 ± 0.059	2.838IleLys: 2.838 ± 0.046	6.776IleLeu: 6.776 ± 0.07	1.635IleMet: 1.635 ± 0.031	2.624IleAsn: 2.624 ± 0.041	3.184IlePro: 3.184 ± 0.049	2.107IleGln: 2.107 ± 0.038	3.814IleArg: 3.814 ± 0.046	4.683IleSer: 4.683 ± 0.052	4.035IleThr: 4.035 ± 0.048	4.534IleVal: 4.534 ± 0.051	0.619IleTrp: 0.619 ± 0.021	1.829IleTyr: 1.829 ± 0.037	0.0IleXaa: 0.0 ± 0.0
Lys	4.218LysAla: 4.218 ± 0.064	0.452LysCys: 0.452 ± 0.017	2.673LysAsp: 2.673 ± 0.045	3.858LysGlu: 3.858 ± 0.053	1.52LysPhe: 1.52 ± 0.031	3.655LysGly: 3.655 ± 0.048	0.93LysHis: 0.93 ± 0.025	3.697LysIle: 3.697 ± 0.048	3.73LysLys: 3.73 ± 0.061	4.502LysLeu: 4.502 ± 0.051	1.708LysMet: 1.708 ± 0.034	2.298LysAsn: 2.298 ± 0.038	2.039LysPro: 2.039 ± 0.031	1.902LysGln: 1.902 ± 0.038	2.76LysArg: 2.76 ± 0.038	2.954LysSer: 2.954 ± 0.043	2.989LysThr: 2.989 ± 0.039	3.6LysVal: 3.6 ± 0.053	0.505LysTrp: 0.505 ± 0.017	1.493LysTyr: 1.493 ± 0.028	0.0LysXaa: 0.0 ± 0.0
Leu	10.097LeuAla: 10.097 ± 0.078	1.247LeuCys: 1.247 ± 0.025	5.274LeuAsp: 5.274 ± 0.061	6.364LeuGlu: 6.364 ± 0.074	4.444LeuPhe: 4.444 ± 0.072	7.265LeuGly: 7.265 ± 0.075	2.122LeuHis: 2.122 ± 0.037	6.129LeuIle: 6.129 ± 0.072	5.148LeuLys: 5.148 ± 0.059	11.237LeuLeu: 11.237 ± 0.11	2.483LeuMet: 2.483 ± 0.04	3.508LeuAsn: 3.508 ± 0.048	5.012LeuPro: 5.012 ± 0.063	4.312LeuGln: 4.312 ± 0.052	5.276LeuArg: 5.276 ± 0.064	6.754LeuSer: 6.754 ± 0.066	5.77LeuThr: 5.77 ± 0.051	7.055LeuVal: 7.055 ± 0.079	0.912LeuTrp: 0.912 ± 0.024	2.709LeuTyr: 2.709 ± 0.042	0.0LeuXaa: 0.0 ± 0.0
Met	2.794MetAla: 2.794 ± 0.045	0.243MetCys: 0.243 ± 0.012	1.383MetAsp: 1.383 ± 0.029	1.757MetGlu: 1.757 ± 0.032	0.908MetPhe: 0.908 ± 0.025	1.949MetGly: 1.949 ± 0.039	0.519MetHis: 0.519 ± 0.017	1.676MetIle: 1.676 ± 0.032	1.761MetLys: 1.761 ± 0.036	2.845MetLeu: 2.845 ± 0.044	0.785MetMet: 0.785 ± 0.024	1.169MetAsn: 1.169 ± 0.029	1.24MetPro: 1.24 ± 0.025	1.071MetGln: 1.071 ± 0.022	1.424MetArg: 1.424 ± 0.027	1.693MetSer: 1.693 ± 0.031	1.709MetThr: 1.709 ± 0.034	2.161MetVal: 2.161 ± 0.036	0.188MetTrp: 0.188 ± 0.01	0.558MetTyr: 0.558 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.573AsnAla: 2.573 ± 0.043	0.575AsnCys: 0.575 ± 0.019	1.924AsnAsp: 1.924 ± 0.033	2.071AsnGlu: 2.071 ± 0.039	1.423AsnPhe: 1.423 ± 0.028	2.816AsnGly: 2.816 ± 0.046	0.825AsnHis: 0.825 ± 0.022	2.97AsnIle: 2.97 ± 0.039	1.542AsnLys: 1.542 ± 0.028	3.837AsnLeu: 3.837 ± 0.056	1.027AsnMet: 1.027 ± 0.024	1.565AsnAsn: 1.565 ± 0.037	2.033AsnPro: 2.033 ± 0.035	1.246AsnGln: 1.246 ± 0.032	2.269AsnArg: 2.269 ± 0.038	2.288AsnSer: 2.288 ± 0.035	2.002AsnThr: 2.002 ± 0.036	2.403AsnVal: 2.403 ± 0.041	0.466AsnTrp: 0.466 ± 0.016	1.152AsnTyr: 1.152 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	3.685ProAla: 3.685 ± 0.049	0.461ProCys: 0.461 ± 0.016	2.748ProAsp: 2.748 ± 0.039	3.733ProGlu: 3.733 ± 0.051	1.957ProPhe: 1.957 ± 0.034	3.385ProGly: 3.385 ± 0.051	0.879ProHis: 0.879 ± 0.025	2.41ProIle: 2.41 ± 0.036	1.862ProLys: 1.862 ± 0.035	4.161ProLeu: 4.161 ± 0.052	1.076ProMet: 1.076 ± 0.023	1.258ProAsn: 1.258 ± 0.029	1.835ProPro: 1.835 ± 0.035	1.544ProGln: 1.544 ± 0.029	1.742ProArg: 1.742 ± 0.036	2.309ProSer: 2.309 ± 0.038	2.056ProThr: 2.056 ± 0.043	3.559ProVal: 3.559 ± 0.045	0.499ProTrp: 0.499 ± 0.022	1.275ProTyr: 1.275 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.271GlnAla: 3.271 ± 0.048	0.396GlnCys: 0.396 ± 0.016	1.694GlnAsp: 1.694 ± 0.033	2.631GlnGlu: 2.631 ± 0.042	1.345GlnPhe: 1.345 ± 0.028	2.451GlnGly: 2.451 ± 0.036	0.906GlnHis: 0.906 ± 0.024	2.314GlnIle: 2.314 ± 0.039	2.273GlnLys: 2.273 ± 0.043	4.134GlnLeu: 4.134 ± 0.06	1.045GlnMet: 1.045 ± 0.026	1.356GlnAsn: 1.356 ± 0.031	1.557GlnPro: 1.557 ± 0.035	1.886GlnGln: 1.886 ± 0.042	2.027GlnArg: 2.027 ± 0.035	2.012GlnSer: 2.012 ± 0.032	1.803GlnThr: 1.803 ± 0.034	2.597GlnVal: 2.597 ± 0.039	0.386GlnTrp: 0.386 ± 0.016	1.02GlnTyr: 1.02 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	3.65ArgAla: 3.65 ± 0.049	0.662ArgCys: 0.662 ± 0.02	2.884ArgAsp: 2.884 ± 0.043	4.0ArgGlu: 4.0 ± 0.052	2.409ArgPhe: 2.409 ± 0.04	3.134ArgGly: 3.134 ± 0.048	1.227ArgHis: 1.227 ± 0.025	3.896ArgIle: 3.896 ± 0.054	3.28ArgLys: 3.28 ± 0.044	5.828ArgLeu: 5.828 ± 0.07	1.568ArgMet: 1.568 ± 0.028	2.133ArgAsn: 2.133 ± 0.037	2.089ArgPro: 2.089 ± 0.036	2.473ArgGln: 2.473 ± 0.041	3.132ArgArg: 3.132 ± 0.052	3.119ArgSer: 3.119 ± 0.045	2.668ArgThr: 2.668 ± 0.039	3.478ArgVal: 3.478 ± 0.046	0.588ArgTrp: 0.588 ± 0.021	1.891ArgTyr: 1.891 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	4.806SerAla: 4.806 ± 0.056	0.944SerCys: 0.944 ± 0.027	2.998SerAsp: 2.998 ± 0.038	3.666SerGlu: 3.666 ± 0.048	2.84SerPhe: 2.84 ± 0.045	5.373SerGly: 5.373 ± 0.069	1.35SerHis: 1.35 ± 0.025	4.069SerIle: 4.069 ± 0.051	2.591SerLys: 2.591 ± 0.044	6.5SerLeu: 6.5 ± 0.068	1.743SerMet: 1.743 ± 0.031	1.915SerAsn: 1.915 ± 0.037	2.692SerPro: 2.692 ± 0.046	2.195SerGln: 2.195 ± 0.039	3.523SerArg: 3.523 ± 0.051	4.145SerSer: 4.145 ± 0.053	3.333SerThr: 3.333 ± 0.046	4.128SerVal: 4.128 ± 0.051	0.803SerTrp: 0.803 ± 0.024	1.832SerTyr: 1.832 ± 0.036	0.0SerXaa: 0.0 ± 0.0
Thr	4.78ThrAla: 4.78 ± 0.059	0.681ThrCys: 0.681 ± 0.023	2.853ThrAsp: 2.853 ± 0.048	3.213ThrGlu: 3.213 ± 0.046	2.352ThrPhe: 2.352 ± 0.038	4.86ThrGly: 4.86 ± 0.062	1.058ThrHis: 1.058 ± 0.026	4.264ThrIle: 4.264 ± 0.057	2.153ThrLys: 2.153 ± 0.034	5.746ThrLeu: 5.746 ± 0.067	1.457ThrMet: 1.457 ± 0.029	1.791ThrAsn: 1.791 ± 0.033	2.723ThrPro: 2.723 ± 0.045	1.505ThrGln: 1.505 ± 0.028	2.708ThrArg: 2.708 ± 0.042	3.395ThrSer: 3.395 ± 0.05	3.327ThrThr: 3.327 ± 0.046	4.195ThrVal: 4.195 ± 0.048	0.535ThrTrp: 0.535 ± 0.019	1.412ThrTyr: 1.412 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	6.288ValAla: 6.288 ± 0.075	0.908ValCys: 0.908 ± 0.029	4.024ValAsp: 4.024 ± 0.049	4.679ValGlu: 4.679 ± 0.054	2.783ValPhe: 2.783 ± 0.046	4.688ValGly: 4.688 ± 0.061	1.373ValHis: 1.373 ± 0.03	4.799ValIle: 4.799 ± 0.051	3.369ValLys: 3.369 ± 0.048	6.969ValLeu: 6.969 ± 0.068	1.947ValMet: 1.947 ± 0.036	2.594ValAsn: 2.594 ± 0.042	2.778ValPro: 2.778 ± 0.042	2.336ValGln: 2.336 ± 0.037	3.607ValArg: 3.607 ± 0.05	4.483ValSer: 4.483 ± 0.052	3.975ValThr: 3.975 ± 0.058	5.47ValVal: 5.47 ± 0.065	0.642ValTrp: 0.642 ± 0.02	1.884ValTyr: 1.884 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.745TrpAla: 0.745 ± 0.023	0.147TrpCys: 0.147 ± 0.01	0.498TrpAsp: 0.498 ± 0.018	0.587TrpGlu: 0.587 ± 0.019	0.504TrpPhe: 0.504 ± 0.021	0.668TrpGly: 0.668 ± 0.023	0.233TrpHis: 0.233 ± 0.01	0.643TrpIle: 0.643 ± 0.02	0.54TrpLys: 0.54 ± 0.018	1.288TrpLeu: 1.288 ± 0.03	0.284TrpMet: 0.284 ± 0.012	0.435TrpAsn: 0.435 ± 0.015	0.422TrpPro: 0.422 ± 0.016	0.651TrpGln: 0.651 ± 0.019	0.615TrpArg: 0.615 ± 0.017	0.644TrpSer: 0.644 ± 0.021	0.464TrpThr: 0.464 ± 0.018	0.682TrpVal: 0.682 ± 0.02	0.148TrpTrp: 0.148 ± 0.009	0.33TrpTyr: 0.33 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.037TyrAla: 2.037 ± 0.035	0.443TyrCys: 0.443 ± 0.016	1.625TyrAsp: 1.625 ± 0.031	1.616TyrGlu: 1.616 ± 0.029	1.406TyrPhe: 1.406 ± 0.033	2.283TyrGly: 2.283 ± 0.039	0.72TyrHis: 0.72 ± 0.021	1.66TyrIle: 1.66 ± 0.033	1.128TyrLys: 1.128 ± 0.029	3.302TyrLeu: 3.302 ± 0.047	0.62TyrMet: 0.62 ± 0.017	1.095TyrAsn: 1.095 ± 0.027	1.367TyrPro: 1.367 ± 0.029	1.205TyrGln: 1.205 ± 0.026	1.959TyrArg: 1.959 ± 0.033	2.021TyrSer: 2.021 ± 0.036	1.639TyrThr: 1.639 ± 0.029	1.744TyrVal: 1.744 ± 0.032	0.343TyrTrp: 0.343 ± 0.013	1.001TyrTyr: 1.001 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5269 proteins (1731742 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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