Novibacillus thermophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Novibacillus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9K710|A0A1U9K710_9BACL Methionine import ATP-binding protein MetN OS=Novibacillus thermophilus OX=1471761 GN=metN PE=3 SV=1
MM1 pKa = 7.26EE2 pKa = 5.5KK3 pKa = 10.32KK4 pKa = 10.84GEE6 pKa = 4.07FYY8 pKa = 10.91LKK10 pKa = 10.08YY11 pKa = 10.96VKK13 pKa = 9.15ITGLLFIIFALALVGCGSSQDD34 pKa = 4.31DD35 pKa = 3.69SGADD39 pKa = 3.39SNEE42 pKa = 3.88GAEE45 pKa = 4.22EE46 pKa = 3.89TDD48 pKa = 3.57AGSEE52 pKa = 4.14DD53 pKa = 4.12VVEE56 pKa = 4.97GDD58 pKa = 3.68TPDD61 pKa = 3.79FLSMLTGGTSGTYY74 pKa = 9.52YY75 pKa = 10.48PLGGEE80 pKa = 4.04MASIITQEE88 pKa = 4.04TGIQTDD94 pKa = 3.66AVSSNASADD103 pKa = 3.58NIMAVHH109 pKa = 6.29NGEE112 pKa = 4.39AEE114 pKa = 4.21LAFTQTDD121 pKa = 3.36VMANAAEE128 pKa = 4.27GVNAFDD134 pKa = 5.07EE135 pKa = 5.2PIEE138 pKa = 4.35DD139 pKa = 3.59VLAIGSLYY147 pKa = 10.17PEE149 pKa = 5.24TIQIVTTADD158 pKa = 3.0SGINSVEE165 pKa = 4.0DD166 pKa = 3.58LAGKK170 pKa = 7.19TVSVGAPGSGTYY182 pKa = 10.16INAEE186 pKa = 3.98QILEE190 pKa = 4.15VHH192 pKa = 6.3GMTMDD197 pKa = 6.51DD198 pKa = 4.57IDD200 pKa = 4.42AQNLDD205 pKa = 3.86FGEE208 pKa = 4.51STGGIQDD215 pKa = 3.77GNIDD219 pKa = 3.72AAFITAGTPTGAVEE233 pKa = 4.36GLTATTDD240 pKa = 3.11ISIVPVAADD249 pKa = 3.23KK250 pKa = 11.59AEE252 pKa = 3.95ALIEE256 pKa = 4.26KK257 pKa = 9.31YY258 pKa = 9.84PYY260 pKa = 9.8YY261 pKa = 10.49ATDD264 pKa = 3.3VVSAGTYY271 pKa = 9.97GLEE274 pKa = 3.95EE275 pKa = 4.48DD276 pKa = 4.3VEE278 pKa = 4.96TVAVLAMLAVTDD290 pKa = 4.37SLSEE294 pKa = 4.03DD295 pKa = 3.38LVYY298 pKa = 10.85DD299 pKa = 3.37ITKK302 pKa = 10.51AIYY305 pKa = 10.16EE306 pKa = 4.13NTEE309 pKa = 3.87NMSHH313 pKa = 6.53AKK315 pKa = 10.57AEE317 pKa = 4.62FIQVEE322 pKa = 4.27TALDD326 pKa = 4.51GIGIDD331 pKa = 3.64LHH333 pKa = 6.76PGAEE337 pKa = 4.26KK338 pKa = 10.85YY339 pKa = 8.47FTEE342 pKa = 5.04AGVLEE347 pKa = 4.37

Molecular weight: 36.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9K5F7|A0A1U9K5F7_9BACL Uncharacterized protein OS=Novibacillus thermophilus OX=1471761 GN=B0W44_05315 PE=4 SV=1
MM1 pKa = 7.4NGRR4 pKa = 11.84RR5 pKa = 11.84MLWTTLIGAAITVIARR21 pKa = 11.84AKK23 pKa = 9.86RR24 pKa = 11.84HH25 pKa = 5.23RR26 pKa = 11.84QAPLARR32 pKa = 11.84LFRR35 pKa = 11.84GAGRR39 pKa = 11.84GNKK42 pKa = 7.74MLWMRR47 pKa = 11.84YY48 pKa = 4.68TQPVMHH54 pKa = 6.72MVRR57 pKa = 11.84RR58 pKa = 11.84MARR61 pKa = 3.08

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3169

0

3169

951385

27

1459

300.2

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.918 ± 0.041

0.793 ± 0.013

5.445 ± 0.032

7.241 ± 0.05

4.111 ± 0.033

7.362 ± 0.04

2.362 ± 0.022

6.143 ± 0.038

5.253 ± 0.037

9.835 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.019

3.386 ± 0.027

4.152 ± 0.028

3.74 ± 0.024

5.858 ± 0.045

5.493 ± 0.028

5.419 ± 0.028

8.307 ± 0.042

1.293 ± 0.019

3.247 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski