Aquimonas voraii
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3593 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6X711|A0A1G6X711_9GAMM DNA polymerase IV OS=Aquimonas voraii OX=265719 GN=dinB PE=3 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 RR3 pKa = 11.84 QSPRR7 pKa = 11.84 KK8 pKa = 9.59 SLVWLCGSLIGGLGAWSALPAQNAAALEE36 pKa = 4.19 YY37 pKa = 10.14 EE38 pKa = 4.21 FAEE41 pKa = 4.0 IHH43 pKa = 5.61 EE44 pKa = 4.98 AEE46 pKa = 4.46 ALAPPGKK53 pKa = 9.61 PGAPSMGSPLGAAPALLGMGFDD75 pKa = 5.88 GEE77 pKa = 4.4 IEE79 pKa = 4.41 PNNSSATASPLAEE92 pKa = 3.79 RR93 pKa = 11.84 QGVVRR98 pKa = 11.84 AKK100 pKa = 9.98 LWPAADD106 pKa = 3.18 IDD108 pKa = 4.18 VFAIAAQAGDD118 pKa = 3.59 RR119 pKa = 11.84 LYY121 pKa = 11.35 AGVMTSASAGSGTDD135 pKa = 2.95 SLLQVIAPDD144 pKa = 3.47 GTTVIEE150 pKa = 4.57 ADD152 pKa = 4.01 DD153 pKa = 4.17 NDD155 pKa = 3.8 GSFAGTSSGVAGTTLTSAGTYY176 pKa = 8.59 YY177 pKa = 10.78 LQVSSPSATATVRR190 pKa = 11.84 PYY192 pKa = 9.98 EE193 pKa = 3.76 LHH195 pKa = 6.25 YY196 pKa = 10.44 RR197 pKa = 11.84 LQAGSPTAEE206 pKa = 3.94 VEE208 pKa = 4.42 PNDD211 pKa = 3.74 TAATANPLPASGWVSGARR229 pKa = 11.84 GIAAATEE236 pKa = 3.99 QDD238 pKa = 3.84 WYY240 pKa = 11.44 SLSLNAGDD248 pKa = 4.3 TVFLSLDD255 pKa = 3.83 LDD257 pKa = 3.91 PEE259 pKa = 4.41 RR260 pKa = 11.84 DD261 pKa = 3.49 NVQWDD266 pKa = 3.41 GRR268 pKa = 11.84 LGLGLFGDD276 pKa = 4.81 ASNQILVANDD286 pKa = 3.25 TSTGSVANPLSEE298 pKa = 4.16 TLFITVKK305 pKa = 10.42 EE306 pKa = 4.01 AGTYY310 pKa = 9.58 FAFVDD315 pKa = 3.87 SANAAVGGPTATYY328 pKa = 9.03 QLSVSVQPAANEE340 pKa = 4.06 GLNCTTYY347 pKa = 10.54 TSTDD351 pKa = 3.28 VPVAIGPNPGLATSTITIPGNPRR374 pKa = 11.84 IADD377 pKa = 3.85 LDD379 pKa = 3.66 VSLQLNHH386 pKa = 6.79 AFMNDD391 pKa = 1.87 IDD393 pKa = 4.19 AQLVSPAGNSVGLFTDD409 pKa = 3.63 IGATSAGGQAQMDD422 pKa = 4.0 TVFDD426 pKa = 4.41 DD427 pKa = 4.27 EE428 pKa = 4.82 AAIPPSFTALRR439 pKa = 11.84 GLALKK444 pKa = 10.61 PEE446 pKa = 4.43 LNYY449 pKa = 10.71 RR450 pKa = 11.84 LAWFDD455 pKa = 3.36 GTDD458 pKa = 4.27 AGGTWTLEE466 pKa = 3.94 LRR468 pKa = 11.84 DD469 pKa = 4.61 DD470 pKa = 4.72 ANTDD474 pKa = 3.35 GGNLTGWSLRR484 pKa = 11.84 VCEE487 pKa = 4.86 APPPPTCAAGFTPVTVYY504 pKa = 9.84 STDD507 pKa = 3.8 FEE509 pKa = 5.68 AGAAGFTSSGIANEE523 pKa = 4.2 WEE525 pKa = 4.18 LGLPATAGTTTTNPIAAFSSCASGSSCWKK554 pKa = 8.72 TDD556 pKa = 3.52 LDD558 pKa = 3.8 GTYY561 pKa = 10.6 DD562 pKa = 3.63 LSSSQDD568 pKa = 3.64 LLSPNIDD575 pKa = 3.25 LSGLQPPVLVRR586 pKa = 11.84 WSQRR590 pKa = 11.84 YY591 pKa = 7.35 QMEE594 pKa = 4.17 SANFDD599 pKa = 3.66 RR600 pKa = 11.84 YY601 pKa = 10.35 HH602 pKa = 7.46 VDD604 pKa = 3.66 FRR606 pKa = 11.84 QVGSGSGLRR615 pKa = 11.84 LFEE618 pKa = 4.15 WADD621 pKa = 3.07 ATMANAVGNPTLQIPAAAGWSQKK644 pKa = 8.78 LVRR647 pKa = 11.84 ADD649 pKa = 3.66 ALAGQNTEE657 pKa = 4.76 LLFHH661 pKa = 7.19 LDD663 pKa = 2.91 SDD665 pKa = 4.17 TTVNFAGVAIDD676 pKa = 4.18 DD677 pKa = 4.24 VSVTACRR684 pKa = 11.84 ALSADD689 pKa = 3.89 LAITKK694 pKa = 9.44 TNGSTTSVPGGSTTYY709 pKa = 10.52 TITSSNAGPDD719 pKa = 3.49 AVTGATVTDD728 pKa = 4.17 TFPAALTCSWTCVGAGGGTCTAASSGNLNDD758 pKa = 4.78 SVNLPAGASVTHH770 pKa = 6.77 TATCAIAAAATGTLSNTATISSTITDD796 pKa = 3.73 PTPANNSATDD806 pKa = 3.56 SDD808 pKa = 4.4 TLSPQADD815 pKa = 3.63 LSITKK820 pKa = 9.51 TDD822 pKa = 3.24 GQTAVPAGGSTVYY835 pKa = 10.62 TITASNAGPSDD846 pKa = 3.86 APGSTVADD854 pKa = 4.09 TLPAACTSVSWTCVAAGGGTCTAAGSGNIGDD885 pKa = 4.64 AANLPAGASVSYY897 pKa = 9.05 TASCAIAAAASGTLSNTATVSSAVTDD923 pKa = 3.68 PTPGNNAATDD933 pKa = 3.82 SSSVLPPGALGASPSTLSFGTVSVGATSASQTVTLSNSGGSPLTIASLGAAAAPFALVGGSCGAVPITLAAGASCTLAYY1012 pKa = 10.48 SFSPSAAGLATQSLTVNAGSAGSASLALSGIGGVGSLGLLSSSLDD1057 pKa = 3.5 FGRR1060 pKa = 11.84 VQVGAGGQLSLTLTNTGSGGLDD1082 pKa = 3.18 VTGLSAVSAPFAQITGGSCGVVPFSLAAGTSCTVLYY1118 pKa = 10.48 RR1119 pKa = 11.84 FAPTATGSFNQTVTVSSTAGSATANLVGIGFVVTAVDD1156 pKa = 3.75 AMDD1159 pKa = 4.05 ARR1161 pKa = 11.84 SLSLLALILGLIGWVTLRR1179 pKa = 11.84 RR1180 pKa = 11.84 RR1181 pKa = 11.84 GG1182 pKa = 3.37
Molecular weight: 117.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.935
IPC_protein 3.986
Toseland 3.757
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.923
Rodwell 3.808
Grimsley 3.656
Solomon 3.973
Lehninger 3.935
Nozaki 4.088
DTASelect 4.368
Thurlkill 3.795
EMBOSS 3.923
Sillero 4.101
Patrickios 1.939
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 3.999
Protein with the highest isoelectric point:
>tr|A0A1G6YWL0|A0A1G6YWL0_9GAMM Uncharacterized protein OS=Aquimonas voraii OX=265719 GN=SAMN04488509_11168 PE=4 SV=1
MM1 pKa = 7.69 LIAKK5 pKa = 9.04 PVGRR9 pKa = 11.84 VFARR13 pKa = 11.84 EE14 pKa = 3.4 RR15 pKa = 11.84 GRR17 pKa = 11.84 RR18 pKa = 11.84 HH19 pKa = 5.25 HH20 pKa = 6.81 HH21 pKa = 5.92 PLRR24 pKa = 11.84 LRR26 pKa = 11.84 RR27 pKa = 11.84 PPPRR31 pKa = 11.84 HH32 pKa = 5.34 RR33 pKa = 11.84 HH34 pKa = 5.01 RR35 pKa = 11.84 PPDD38 pKa = 3.08 RR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 GLCLQRR48 pKa = 11.84 RR49 pKa = 11.84 GRR51 pKa = 11.84 AARR54 pKa = 11.84 AHH56 pKa = 6.39 RR57 pKa = 11.84 PRR59 pKa = 11.84 RR60 pKa = 11.84 PAHH63 pKa = 6.79 RR64 pKa = 11.84 DD65 pKa = 2.86 RR66 pKa = 11.84 ARR68 pKa = 11.84 RR69 pKa = 11.84 PGSHH73 pKa = 6.11 HH74 pKa = 6.75 RR75 pKa = 11.84 APLRR79 pKa = 11.84 RR80 pKa = 11.84 SPKK83 pKa = 9.72 RR84 pKa = 11.84 RR85 pKa = 11.84 GQPRR89 pKa = 11.84 GLYY92 pKa = 10.17 AGWRR96 pKa = 11.84 RR97 pKa = 11.84 PANHH101 pKa = 7.13 AEE103 pKa = 3.83 PVRR106 pKa = 11.84 RR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 RR110 pKa = 11.84 PAPRR114 pKa = 11.84 AAPLRR119 pKa = 11.84 RR120 pKa = 11.84 PGPPDD125 pKa = 3.12 RR126 pKa = 11.84 RR127 pKa = 11.84 RR128 pKa = 11.84 RR129 pKa = 11.84 PLQPAQHH136 pKa = 6.64 LDD138 pKa = 3.46 LRR140 pKa = 11.84 RR141 pKa = 11.84 SRR143 pKa = 11.84 QPPEE147 pKa = 3.48 RR148 pKa = 11.84 DD149 pKa = 3.34 GPGRR153 pKa = 11.84 HH154 pKa = 5.08 HH155 pKa = 6.76 HH156 pKa = 6.49 HH157 pKa = 7.03 RR158 pKa = 11.84 AGSPQPHH165 pKa = 6.83 RR166 pKa = 11.84 PPDD169 pKa = 3.53 HH170 pKa = 6.77 GRR172 pKa = 11.84 GQHH175 pKa = 6.22 GAQLQQRR182 pKa = 11.84 RR183 pKa = 11.84 PPATDD188 pKa = 3.27 HH189 pKa = 6.1 PPEE192 pKa = 4.51 RR193 pKa = 11.84 RR194 pKa = 11.84 AQRR197 pKa = 11.84 NPLRR201 pKa = 11.84 RR202 pKa = 11.84 RR203 pKa = 11.84 RR204 pKa = 11.84 PHH206 pKa = 6.72 RR207 pKa = 11.84 AHH209 pKa = 6.87 HH210 pKa = 6.45 PPPGQQRR217 pKa = 11.84 SRR219 pKa = 11.84 ALRR222 pKa = 11.84 IRR224 pKa = 11.84 LRR226 pKa = 11.84 RR227 pKa = 11.84 ARR229 pKa = 11.84 QPTT232 pKa = 3.36
Molecular weight: 27.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 10.862
IPC_protein 12.457
Toseland 12.618
ProMoST 13.115
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.12
Grimsley 12.647
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 11.828
IPC_peptide 13.115
IPC2_peptide 12.106
IPC2.peptide.svr19 9.159
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3593
0
3593
1297485
39
3779
361.1
39.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.453 ± 0.058
0.906 ± 0.013
5.336 ± 0.029
6.103 ± 0.044
3.407 ± 0.025
8.641 ± 0.044
2.028 ± 0.019
3.95 ± 0.029
2.126 ± 0.029
11.743 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.842 ± 0.017
2.318 ± 0.028
5.483 ± 0.031
3.76 ± 0.027
8.237 ± 0.054
5.699 ± 0.04
4.462 ± 0.049
7.043 ± 0.036
1.442 ± 0.017
2.022 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here