Klebsiella phage vB_KpnM_KpS110
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9VAH8|A0A2K9VAH8_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_KpS110 OX=2079262 GN=kps110_093 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.23 LNKK5 pKa = 9.49 ILLVCALAFSTTACSTLLDD24 pKa = 4.01 VPDD27 pKa = 4.4 LSGPDD32 pKa = 3.58 FTQDD36 pKa = 3.04 QAQTKK41 pKa = 8.7 MDD43 pKa = 4.05 DD44 pKa = 3.43 MVKK47 pKa = 10.46 AHH49 pKa = 7.14 AALQGTTPGPIQTVCNYY66 pKa = 10.46 DD67 pKa = 3.73 DD68 pKa = 4.68 SDD70 pKa = 3.91 PTTEE74 pKa = 5.24 LYY76 pKa = 10.56 HH77 pKa = 6.97 CSTFVKK83 pKa = 10.55 DD84 pKa = 4.05 SMVVLYY90 pKa = 10.14 GDD92 pKa = 4.43 CQADD96 pKa = 3.94 GCKK99 pKa = 9.49 ATGYY103 pKa = 11.13 DD104 pKa = 4.02 KK105 pKa = 11.61 VEE107 pKa = 3.94 EE108 pKa = 4.53 DD109 pKa = 3.61 KK110 pKa = 11.58
Molecular weight: 11.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.895
IPC2_protein 4.151
IPC_protein 4.126
Toseland 3.897
ProMoST 4.317
Dawson 4.139
Bjellqvist 4.291
Wikipedia 4.113
Rodwell 3.948
Grimsley 3.808
Solomon 4.126
Lehninger 4.088
Nozaki 4.253
DTASelect 4.558
Thurlkill 3.961
EMBOSS 4.113
Sillero 4.253
Patrickios 1.036
IPC_peptide 4.113
IPC2_peptide 4.228
IPC2.peptide.svr19 4.117
Protein with the highest isoelectric point:
>tr|A0A2K9VAF4|A0A2K9VAF4_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnM_KpS110 OX=2079262 GN=kps110_073 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 YY3 pKa = 9.1 VDD5 pKa = 5.12 RR6 pKa = 11.84 MLGQNEE12 pKa = 4.03 HH13 pKa = 6.68 VIGFTRR19 pKa = 11.84 PTWWSGFWVYY29 pKa = 9.63 FWVAVFLVPTFGISLFFLIPTVIRR53 pKa = 11.84 NLTTEE58 pKa = 4.07 FAVTNKK64 pKa = 10.01 RR65 pKa = 11.84 VIFKK69 pKa = 9.78 TGFIRR74 pKa = 11.84 RR75 pKa = 11.84 DD76 pKa = 3.26 ADD78 pKa = 3.45 EE79 pKa = 4.56 LRR81 pKa = 11.84 LGKK84 pKa = 10.36 VEE86 pKa = 4.17 TVKK89 pKa = 10.36 VDD91 pKa = 3.01 QSITGRR97 pKa = 11.84 VLRR100 pKa = 11.84 FSTISVIGTGGTRR113 pKa = 11.84 LVAKK117 pKa = 10.44 GCAKK121 pKa = 10.68 GNDD124 pKa = 3.86 FRR126 pKa = 11.84 RR127 pKa = 11.84 VIYY130 pKa = 10.25 DD131 pKa = 3.17 QLDD134 pKa = 3.3 NN135 pKa = 4.17
Molecular weight: 15.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 9.516
IPC_protein 10.058
Toseland 10.452
ProMoST 10.101
Dawson 10.584
Bjellqvist 10.262
Wikipedia 10.76
Rodwell 10.847
Grimsley 10.628
Solomon 10.657
Lehninger 10.628
Nozaki 10.438
DTASelect 10.262
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.496
Patrickios 10.599
IPC_peptide 10.657
IPC2_peptide 9.077
IPC2.peptide.svr19 8.662
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
201
0
201
48268
38
1612
240.1
27.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.644 ± 0.193
1.218 ± 0.07
6.487 ± 0.122
6.516 ± 0.192
4.303 ± 0.12
6.655 ± 0.163
1.821 ± 0.105
6.335 ± 0.12
6.491 ± 0.192
7.96 ± 0.14
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.867 ± 0.101
5.248 ± 0.114
3.829 ± 0.123
3.779 ± 0.113
4.887 ± 0.118
6.514 ± 0.188
5.95 ± 0.228
6.998 ± 0.16
1.49 ± 0.07
4.009 ± 0.107
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here