Amino acid dipepetide frequency for Staphylococcus phage phiSA_BS2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.487AlaAla: 0.487 ± 0.126
0.146AlaCys: 0.146 ± 0.059
1.973AlaAsp: 1.973 ± 0.205
3.07AlaGlu: 3.07 ± 0.315
1.876AlaPhe: 1.876 ± 0.232
1.876AlaGly: 1.876 ± 0.331
0.707AlaHis: 0.707 ± 0.129
3.021AlaIle: 3.021 ± 0.279
4.215AlaLys: 4.215 ± 0.327
3.045AlaLeu: 3.045 ± 0.318
0.901AlaMet: 0.901 ± 0.192
1.754AlaAsn: 1.754 ± 0.211
1.34AlaPro: 1.34 ± 0.247
1.511AlaGln: 1.511 ± 0.222
1.608AlaArg: 1.608 ± 0.185
2.753AlaSer: 2.753 ± 0.302
2.314AlaThr: 2.314 ± 0.222
2.314AlaVal: 2.314 ± 0.243
0.414AlaTrp: 0.414 ± 0.1
2.046AlaTyr: 2.046 ± 0.197
0.0AlaXaa: 0.0 ± 0.0
Cys
0.171CysAla: 0.171 ± 0.061
0.024CysCys: 0.024 ± 0.025
0.195CysAsp: 0.195 ± 0.071
0.317CysGlu: 0.317 ± 0.087
0.268CysPhe: 0.268 ± 0.084
0.658CysGly: 0.658 ± 0.129
0.049CysHis: 0.049 ± 0.037
0.341CysIle: 0.341 ± 0.093
0.585CysLys: 0.585 ± 0.138
0.56CysLeu: 0.56 ± 0.103
0.097CysMet: 0.097 ± 0.046
0.414CysAsn: 0.414 ± 0.107
0.414CysPro: 0.414 ± 0.115
0.171CysGln: 0.171 ± 0.063
0.292CysArg: 0.292 ± 0.1
0.39CysSer: 0.39 ± 0.103
0.414CysThr: 0.414 ± 0.113
0.268CysVal: 0.268 ± 0.077
0.073CysTrp: 0.073 ± 0.041
0.414CysTyr: 0.414 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
2.241AspAla: 2.241 ± 0.296
0.317AspCys: 0.317 ± 0.089
3.874AspAsp: 3.874 ± 0.352
5.409AspGlu: 5.409 ± 0.383
3.557AspPhe: 3.557 ± 0.321
3.192AspGly: 3.192 ± 0.227
0.682AspHis: 0.682 ± 0.137
7.309AspIle: 7.309 ± 0.45
7.163AspLys: 7.163 ± 0.395
5.871AspLeu: 5.871 ± 0.368
1.852AspMet: 1.852 ± 0.215
4.897AspAsn: 4.897 ± 0.395
1.827AspPro: 1.827 ± 0.239
1.73AspGln: 1.73 ± 0.204
2.631AspArg: 2.631 ± 0.263
4.361AspSer: 4.361 ± 0.313
4.069AspThr: 4.069 ± 0.325
3.947AspVal: 3.947 ± 0.335
0.536AspTrp: 0.536 ± 0.117
4.264AspTyr: 4.264 ± 0.337
0.0AspXaa: 0.0 ± 0.0
Glu
3.581GluAla: 3.581 ± 0.354
0.463GluCys: 0.463 ± 0.13
7.406GluAsp: 7.406 ± 0.429
10.598GluGlu: 10.598 ± 0.884
3.362GluPhe: 3.362 ± 0.287
5.067GluGly: 5.067 ± 0.363
1.437GluHis: 1.437 ± 0.172
6.286GluIle: 6.286 ± 0.466
7.406GluLys: 7.406 ± 0.574
7.479GluLeu: 7.479 ± 0.514
2.534GluMet: 2.534 ± 0.271
5.969GluAsn: 5.969 ± 0.401
2.193GluPro: 2.193 ± 0.308
4.629GluGln: 4.629 ± 0.385
3.265GluArg: 3.265 ± 0.282
4.873GluSer: 4.873 ± 0.306
4.288GluThr: 4.288 ± 0.327
5.579GluVal: 5.579 ± 0.448
0.877GluTrp: 0.877 ± 0.144
5.067GluTyr: 5.067 ± 0.343
0.0GluXaa: 0.0 ± 0.0
Phe
1.194PheAla: 1.194 ± 0.154
0.365PheCys: 0.365 ± 0.088
3.289PheAsp: 3.289 ± 0.252
3.216PheGlu: 3.216 ± 0.271
1.267PhePhe: 1.267 ± 0.149
2.193PheGly: 2.193 ± 0.287
0.658PheHis: 0.658 ± 0.131
3.021PheIle: 3.021 ± 0.3
3.703PheLys: 3.703 ± 0.273
3.094PheLeu: 3.094 ± 0.291
1.169PheMet: 1.169 ± 0.149
3.776PheAsn: 3.776 ± 0.318
0.853PhePro: 0.853 ± 0.118
1.364PheGln: 1.364 ± 0.202
1.535PheArg: 1.535 ± 0.203
1.998PheSer: 1.998 ± 0.255
2.729PheThr: 2.729 ± 0.269
2.485PheVal: 2.485 ± 0.272
0.292PheTrp: 0.292 ± 0.08
2.144PheTyr: 2.144 ± 0.229
0.0PheXaa: 0.0 ± 0.0
Gly
2.168GlyAla: 2.168 ± 0.36
0.39GlyCys: 0.39 ± 0.093
3.947GlyAsp: 3.947 ± 0.412
4.556GlyGlu: 4.556 ± 0.302
2.607GlyPhe: 2.607 ± 0.325
3.996GlyGly: 3.996 ± 0.833
1.121GlyHis: 1.121 ± 0.188
4.239GlyIle: 4.239 ± 0.378
4.921GlyLys: 4.921 ± 0.468
4.458GlyLeu: 4.458 ± 0.4
1.632GlyMet: 1.632 ± 0.312
4.166GlyAsn: 4.166 ± 0.423
0.024GlyPro: 0.024 ± 0.022
2.193GlyGln: 2.193 ± 0.249
1.876GlyArg: 1.876 ± 0.229
3.63GlySer: 3.63 ± 0.354
3.606GlyThr: 3.606 ± 0.394
3.752GlyVal: 3.752 ± 0.349
0.78GlyTrp: 0.78 ± 0.142
3.338GlyTyr: 3.338 ± 0.319
0.0GlyXaa: 0.0 ± 0.0
His
0.633HisAla: 0.633 ± 0.098
0.244HisCys: 0.244 ± 0.091
0.828HisAsp: 0.828 ± 0.144
0.877HisGlu: 0.877 ± 0.167
0.585HisPhe: 0.585 ± 0.098
0.95HisGly: 0.95 ± 0.165
0.268HisHis: 0.268 ± 0.086
1.218HisIle: 1.218 ± 0.162
1.169HisLys: 1.169 ± 0.216
1.584HisLeu: 1.584 ± 0.233
0.341HisMet: 0.341 ± 0.087
0.999HisAsn: 0.999 ± 0.176
0.439HisPro: 0.439 ± 0.096
0.658HisGln: 0.658 ± 0.123
0.463HisArg: 0.463 ± 0.098
1.048HisSer: 1.048 ± 0.152
0.877HisThr: 0.877 ± 0.134
0.95HisVal: 0.95 ± 0.162
0.195HisTrp: 0.195 ± 0.079
0.877HisTyr: 0.877 ± 0.13
0.0HisXaa: 0.0 ± 0.0
Ile
2.826IleAla: 2.826 ± 0.264
0.365IleCys: 0.365 ± 0.089
6.066IleAsp: 6.066 ± 0.409
6.992IleGlu: 6.992 ± 0.524
2.095IlePhe: 2.095 ± 0.184
4.093IleGly: 4.093 ± 0.295
1.291IleHis: 1.291 ± 0.185
4.897IleIle: 4.897 ± 0.448
7.211IleLys: 7.211 ± 0.437
5.287IleLeu: 5.287 ± 0.444
1.584IleMet: 1.584 ± 0.232
5.409IleAsn: 5.409 ± 0.38
2.461IlePro: 2.461 ± 0.252
2.729IleGln: 2.729 ± 0.208
2.924IleArg: 2.924 ± 0.276
3.898IleSer: 3.898 ± 0.325
4.678IleThr: 4.678 ± 0.335
4.507IleVal: 4.507 ± 0.415
0.487IleTrp: 0.487 ± 0.113
3.313IleTyr: 3.313 ± 0.308
0.0IleXaa: 0.0 ± 0.0
Lys
3.898LysAla: 3.898 ± 0.35
0.487LysCys: 0.487 ± 0.11
7.333LysAsp: 7.333 ± 0.421
11.061LysGlu: 11.061 ± 0.682
2.729LysPhe: 2.729 ± 0.264
5.652LysGly: 5.652 ± 0.508
1.486LysHis: 1.486 ± 0.169
5.482LysIle: 5.482 ± 0.356
7.406LysLys: 7.406 ± 0.512
6.578LysLeu: 6.578 ± 0.372
2.314LysMet: 2.314 ± 0.262
5.433LysAsn: 5.433 ± 0.372
2.656LysPro: 2.656 ± 0.287
3.874LysGln: 3.874 ± 0.354
3.24LysArg: 3.24 ± 0.257
4.921LysSer: 4.921 ± 0.4
5.019LysThr: 5.019 ± 0.36
6.066LysVal: 6.066 ± 0.405
0.463LysTrp: 0.463 ± 0.097
5.214LysTyr: 5.214 ± 0.345
0.0LysXaa: 0.0 ± 0.0
Leu
3.07LeuAla: 3.07 ± 0.29
0.56LeuCys: 0.56 ± 0.119
6.407LeuAsp: 6.407 ± 0.474
7.894LeuGlu: 7.894 ± 0.477
2.704LeuPhe: 2.704 ± 0.232
4.58LeuGly: 4.58 ± 0.433
0.926LeuHis: 0.926 ± 0.143
4.921LeuIle: 4.921 ± 0.403
7.553LeuLys: 7.553 ± 0.477
5.774LeuLeu: 5.774 ± 0.454
1.998LeuMet: 1.998 ± 0.199
6.115LeuAsn: 6.115 ± 0.412
2.144LeuPro: 2.144 ± 0.209
2.753LeuGln: 2.753 ± 0.284
3.484LeuArg: 3.484 ± 0.25
5.311LeuSer: 5.311 ± 0.36
4.483LeuThr: 4.483 ± 0.334
3.533LeuVal: 3.533 ± 0.274
0.56LeuTrp: 0.56 ± 0.108
3.679LeuTyr: 3.679 ± 0.376
0.0LeuXaa: 0.0 ± 0.0
Met
1.291MetAla: 1.291 ± 0.209
0.073MetCys: 0.073 ± 0.037
1.437MetAsp: 1.437 ± 0.177
2.022MetGlu: 2.022 ± 0.24
1.023MetPhe: 1.023 ± 0.176
1.267MetGly: 1.267 ± 0.363
0.219MetHis: 0.219 ± 0.082
1.778MetIle: 1.778 ± 0.262
2.461MetLys: 2.461 ± 0.304
1.973MetLeu: 1.973 ± 0.186
0.731MetMet: 0.731 ± 0.267
1.657MetAsn: 1.657 ± 0.223
0.414MetPro: 0.414 ± 0.096
0.78MetGln: 0.78 ± 0.113
1.096MetArg: 1.096 ± 0.184
1.778MetSer: 1.778 ± 0.218
1.462MetThr: 1.462 ± 0.186
1.145MetVal: 1.145 ± 0.154
0.244MetTrp: 0.244 ± 0.079
1.096MetTyr: 1.096 ± 0.176
0.0MetXaa: 0.0 ± 0.0
Asn
2.436AsnAla: 2.436 ± 0.239
0.487AsnCys: 0.487 ± 0.117
3.922AsnAsp: 3.922 ± 0.31
5.75AsnGlu: 5.75 ± 0.363
3.167AsnPhe: 3.167 ± 0.28
4.41AsnGly: 4.41 ± 0.4
1.145AsnHis: 1.145 ± 0.179
5.384AsnIle: 5.384 ± 0.433
7.187AsnLys: 7.187 ± 0.378
5.409AsnLeu: 5.409 ± 0.383
1.462AsnMet: 1.462 ± 0.179
5.287AsnAsn: 5.287 ± 0.449
2.339AsnPro: 2.339 ± 0.271
2.168AsnGln: 2.168 ± 0.23
2.631AsnArg: 2.631 ± 0.302
4.044AsnSer: 4.044 ± 0.305
4.629AsnThr: 4.629 ± 0.315
3.776AsnVal: 3.776 ± 0.296
0.682AsnTrp: 0.682 ± 0.135
3.46AsnTyr: 3.46 ± 0.302
0.0AsnXaa: 0.0 ± 0.0
Pro
0.731ProAla: 0.731 ± 0.159
0.171ProCys: 0.171 ± 0.064
1.072ProAsp: 1.072 ± 0.188
2.753ProGlu: 2.753 ± 0.367
1.413ProPhe: 1.413 ± 0.2
0.707ProGly: 0.707 ± 0.104
0.487ProHis: 0.487 ± 0.094
1.803ProIle: 1.803 ± 0.229
2.485ProLys: 2.485 ± 0.241
1.973ProLeu: 1.973 ± 0.189
0.39ProMet: 0.39 ± 0.085
1.998ProAsn: 1.998 ± 0.237
0.439ProPro: 0.439 ± 0.106
1.681ProGln: 1.681 ± 0.281
1.048ProArg: 1.048 ± 0.153
1.9ProSer: 1.9 ± 0.225
2.12ProThr: 2.12 ± 0.242
1.34ProVal: 1.34 ± 0.177
0.146ProTrp: 0.146 ± 0.059
1.364ProTyr: 1.364 ± 0.187
0.0ProXaa: 0.0 ± 0.0
Gln
2.436GlnAla: 2.436 ± 0.281
0.146GlnCys: 0.146 ± 0.054
2.485GlnAsp: 2.485 ± 0.265
3.606GlnGlu: 3.606 ± 0.362
1.584GlnPhe: 1.584 ± 0.177
2.802GlnGly: 2.802 ± 0.304
0.56GlnHis: 0.56 ± 0.115
2.29GlnIle: 2.29 ± 0.236
2.753GlnLys: 2.753 ± 0.294
2.924GlnLeu: 2.924 ± 0.279
0.853GlnMet: 0.853 ± 0.135
2.412GlnAsn: 2.412 ± 0.235
1.389GlnPro: 1.389 ± 0.32
2.363GlnGln: 2.363 ± 0.513
1.462GlnArg: 1.462 ± 0.227
2.388GlnSer: 2.388 ± 0.234
1.998GlnThr: 1.998 ± 0.208
2.558GlnVal: 2.558 ± 0.271
0.317GlnTrp: 0.317 ± 0.078
1.535GlnTyr: 1.535 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
1.584ArgAla: 1.584 ± 0.242
0.219ArgCys: 0.219 ± 0.08
2.704ArgAsp: 2.704 ± 0.27
3.533ArgGlu: 3.533 ± 0.32
1.827ArgPhe: 1.827 ± 0.219
2.046ArgGly: 2.046 ± 0.248
0.536ArgHis: 0.536 ± 0.134
2.485ArgIle: 2.485 ± 0.25
3.581ArgLys: 3.581 ± 0.35
3.362ArgLeu: 3.362 ± 0.349
1.121ArgMet: 1.121 ± 0.208
2.412ArgAsn: 2.412 ± 0.229
0.853ArgPro: 0.853 ± 0.147
1.364ArgGln: 1.364 ± 0.177
1.559ArgArg: 1.559 ± 0.205
1.803ArgSer: 1.803 ± 0.189
2.168ArgThr: 2.168 ± 0.297
2.729ArgVal: 2.729 ± 0.264
0.414ArgTrp: 0.414 ± 0.088
2.217ArgTyr: 2.217 ± 0.236
0.0ArgXaa: 0.0 ± 0.0
Ser
2.217SerAla: 2.217 ± 0.288
0.268SerCys: 0.268 ± 0.08
4.58SerAsp: 4.58 ± 0.324
4.702SerGlu: 4.702 ± 0.321
2.363SerPhe: 2.363 ± 0.219
3.971SerGly: 3.971 ± 0.393
0.78SerHis: 0.78 ± 0.151
4.653SerIle: 4.653 ± 0.385
5.433SerLys: 5.433 ± 0.385
4.97SerLeu: 4.97 ± 0.416
1.316SerMet: 1.316 ± 0.28
4.312SerAsn: 4.312 ± 0.319
1.437SerPro: 1.437 ± 0.185
2.266SerGln: 2.266 ± 0.235
1.973SerArg: 1.973 ± 0.195
4.312SerSer: 4.312 ± 0.513
3.484SerThr: 3.484 ± 0.322
3.143SerVal: 3.143 ± 0.296
0.463SerTrp: 0.463 ± 0.114
3.654SerTyr: 3.654 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
2.241ThrAla: 2.241 ± 0.251
0.292ThrCys: 0.292 ± 0.087
3.435ThrAsp: 3.435 ± 0.256
4.824ThrGlu: 4.824 ± 0.403
2.68ThrPhe: 2.68 ± 0.303
3.874ThrGly: 3.874 ± 0.355
1.023ThrHis: 1.023 ± 0.159
5.019ThrIle: 5.019 ± 0.359
5.092ThrLys: 5.092 ± 0.388
4.873ThrLeu: 4.873 ± 0.329
1.194ThrMet: 1.194 ± 0.217
3.971ThrAsn: 3.971 ± 0.296
2.022ThrPro: 2.022 ± 0.253
2.217ThrGln: 2.217 ± 0.268
2.68ThrArg: 2.68 ± 0.282
3.167ThrSer: 3.167 ± 0.31
3.533ThrThr: 3.533 ± 0.399
3.971ThrVal: 3.971 ± 0.352
0.609ThrTrp: 0.609 ± 0.127
2.875ThrTyr: 2.875 ± 0.281
0.0ThrXaa: 0.0 ± 0.0
Val
2.266ValAla: 2.266 ± 0.215
0.609ValCys: 0.609 ± 0.143
4.483ValAsp: 4.483 ± 0.401
5.969ValGlu: 5.969 ± 0.42
2.509ValPhe: 2.509 ± 0.268
2.534ValGly: 2.534 ± 0.202
0.926ValHis: 0.926 ± 0.167
3.898ValIle: 3.898 ± 0.324
5.555ValLys: 5.555 ± 0.458
4.556ValLeu: 4.556 ± 0.35
0.999ValMet: 0.999 ± 0.145
3.996ValAsn: 3.996 ± 0.327
1.608ValPro: 1.608 ± 0.243
2.095ValGln: 2.095 ± 0.311
2.753ValArg: 2.753 ± 0.241
4.117ValSer: 4.117 ± 0.355
3.752ValThr: 3.752 ± 0.335
3.386ValVal: 3.386 ± 0.288
0.39ValTrp: 0.39 ± 0.076
3.167ValTyr: 3.167 ± 0.295
0.0ValXaa: 0.0 ± 0.0
Trp
0.292TrpAla: 0.292 ± 0.098
0.049TrpCys: 0.049 ± 0.035
0.585TrpAsp: 0.585 ± 0.133
0.999TrpGlu: 0.999 ± 0.155
0.292TrpPhe: 0.292 ± 0.092
0.39TrpGly: 0.39 ± 0.099
0.122TrpHis: 0.122 ± 0.055
0.633TrpIle: 0.633 ± 0.127
0.828TrpLys: 0.828 ± 0.15
0.512TrpLeu: 0.512 ± 0.109
0.171TrpMet: 0.171 ± 0.066
0.463TrpAsn: 0.463 ± 0.109
0.0TrpPro: 0.0 ± 0.0
0.439TrpGln: 0.439 ± 0.115
0.317TrpArg: 0.317 ± 0.095
0.633TrpSer: 0.633 ± 0.132
0.365TrpThr: 0.365 ± 0.099
0.609TrpVal: 0.609 ± 0.118
0.171TrpTrp: 0.171 ± 0.067
0.609TrpTyr: 0.609 ± 0.133
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.608TyrAla: 1.608 ± 0.19
0.439TyrCys: 0.439 ± 0.104
3.703TyrAsp: 3.703 ± 0.286
4.215TyrGlu: 4.215 ± 0.327
2.436TyrPhe: 2.436 ± 0.315
3.143TyrGly: 3.143 ± 0.292
0.78TyrHis: 0.78 ± 0.139
4.434TyrIle: 4.434 ± 0.385
4.605TyrLys: 4.605 ± 0.385
4.166TyrLeu: 4.166 ± 0.316
1.267TyrMet: 1.267 ± 0.193
4.337TyrAsn: 4.337 ± 0.391
1.169TyrPro: 1.169 ± 0.199
1.876TyrGln: 1.876 ± 0.209
1.681TyrArg: 1.681 ± 0.193
2.972TyrSer: 2.972 ± 0.265
3.606TyrThr: 3.606 ± 0.32
3.508TyrVal: 3.508 ± 0.274
0.365TyrTrp: 0.365 ± 0.108
3.24TyrTyr: 3.24 ± 0.269
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 206 proteins (41047 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski