Mycobacterium phage Chris

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SZ02|A0A6M3SZ02_9CAUD Uncharacterized protein OS=Mycobacterium phage Chris OX=2725626 GN=48 PE=4 SV=1
MM1 pKa = 7.15SAAARR6 pKa = 11.84AALAAWQAMPSTVVCGPEE24 pKa = 3.46AAEE27 pKa = 4.66AGRR30 pKa = 11.84AMAAALSEE38 pKa = 4.79LLAQPGEE45 pKa = 4.23VWVLDD50 pKa = 3.96VEE52 pKa = 4.79AEE54 pKa = 4.25GPEE57 pKa = 4.2GGDD60 pKa = 3.21YY61 pKa = 11.22DD62 pKa = 4.08GWQSVHH68 pKa = 6.72ASRR71 pKa = 11.84EE72 pKa = 4.11SAIGRR77 pKa = 11.84LVEE80 pKa = 4.07KK81 pKa = 10.4LVEE84 pKa = 4.1YY85 pKa = 10.84GLGDD89 pKa = 3.73VEE91 pKa = 4.52PQGSATADD99 pKa = 3.26NGSISGDD106 pKa = 3.69YY107 pKa = 10.19EE108 pKa = 4.13VGGVSVSYY116 pKa = 10.15GVHH119 pKa = 6.18RR120 pKa = 11.84MPVEE124 pKa = 3.76PP125 pKa = 5.14

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SZA7|A0A6M3SZA7_9CAUD 3'-phosphate/5'-hydroxy nucleic acid ligase OS=Mycobacterium phage Chris OX=2725626 GN=86 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 8.81RR3 pKa = 11.84TKK5 pKa = 10.24VFRR8 pKa = 11.84GPVVEE13 pKa = 4.14VEE15 pKa = 4.15RR16 pKa = 11.84PEE18 pKa = 4.15VVVNGRR24 pKa = 11.84VLEE27 pKa = 4.57PGTEE31 pKa = 3.84VSIAGEE37 pKa = 3.76RR38 pKa = 11.84GRR40 pKa = 11.84YY41 pKa = 9.07RR42 pKa = 11.84FVKK45 pKa = 9.92SARR48 pKa = 11.84ASSGRR53 pKa = 11.84ITCDD57 pKa = 4.24FIGPDD62 pKa = 4.12DD63 pKa = 3.76QTKK66 pKa = 9.74CWRR69 pKa = 11.84SFYY72 pKa = 9.91PEE74 pKa = 4.32RR75 pKa = 11.84IKK77 pKa = 9.98TVHH80 pKa = 6.33RR81 pKa = 11.84LNRR84 pKa = 11.84TRR86 pKa = 11.84ANVAAA91 pKa = 4.93

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

19099

39

1355

191.0

20.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.037 ± 0.472

1.183 ± 0.143

6.54 ± 0.262

5.597 ± 0.335

2.686 ± 0.174

8.828 ± 0.494

2.236 ± 0.143

3.738 ± 0.168

3.22 ± 0.183

8.294 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.104

2.696 ± 0.168

5.917 ± 0.269

3.173 ± 0.118

6.812 ± 0.377

5.194 ± 0.217

5.843 ± 0.18

8.027 ± 0.3

2.094 ± 0.104

2.539 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski