Mycobacterium phage Gail

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D5JV30|A0A7D5JV30_9CAUD Uncharacterized protein OS=Mycobacterium phage Gail OX=2743994 GN=8 PE=4 SV=1
MM1 pKa = 7.48SNSTYY6 pKa = 10.1IIYY9 pKa = 9.47EE10 pKa = 3.86ALEE13 pKa = 4.02NAVYY17 pKa = 9.96EE18 pKa = 4.85AIDD21 pKa = 3.67AGKK24 pKa = 10.25LDD26 pKa = 4.13PEE28 pKa = 4.71ALDD31 pKa = 4.05GLPTSTVFYY40 pKa = 9.74PEE42 pKa = 4.77PGEE45 pKa = 4.08PWDD48 pKa = 4.35EE49 pKa = 4.38DD50 pKa = 3.75DD51 pKa = 3.89VAAIHH56 pKa = 6.09AVGMSDD62 pKa = 4.46LEE64 pKa = 4.29AKK66 pKa = 10.22IGFYY70 pKa = 10.78RR71 pKa = 11.84NLLDD75 pKa = 5.43RR76 pKa = 11.84IGGG79 pKa = 3.69

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D5FUS4|A0A7D5FUS4_9CAUD Uncharacterized protein OS=Mycobacterium phage Gail OX=2743994 GN=57 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 3.5VSFHH6 pKa = 5.76GSARR10 pKa = 11.84LRR12 pKa = 11.84QAVAATQNRR21 pKa = 11.84DD22 pKa = 2.84AFRR25 pKa = 11.84NWLEE29 pKa = 4.09RR30 pKa = 11.84NSASAAPRR38 pKa = 11.84WLSRR42 pKa = 11.84EE43 pKa = 3.67RR44 pKa = 11.84WLHH47 pKa = 7.17DD48 pKa = 3.26VADD51 pKa = 3.92WSTGEE56 pKa = 4.02GFRR59 pKa = 11.84AARR62 pKa = 11.84AASGISMTAPTFMAIMRR79 pKa = 11.84VMADD83 pKa = 3.46HH84 pKa = 7.56ADD86 pKa = 3.74HH87 pKa = 7.64ADD89 pKa = 3.64GRR91 pKa = 11.84CVAVSRR97 pKa = 11.84ARR99 pKa = 11.84IAAAVGCSPRR109 pKa = 11.84TVTNAWRR116 pKa = 11.84LLRR119 pKa = 11.84LSGYY123 pKa = 9.22GLEE126 pKa = 4.38VQRR129 pKa = 11.84GHH131 pKa = 6.9GSEE134 pKa = 3.87QTPSQGCRR142 pKa = 11.84PSIYY146 pKa = 9.97HH147 pKa = 6.37LVSKK151 pKa = 10.34RR152 pKa = 11.84RR153 pKa = 11.84PVVEE157 pKa = 4.73FCYY160 pKa = 10.42LPPKK164 pKa = 10.32AGVGSSSPVDD174 pKa = 3.34QFSLNGAPRR183 pKa = 11.84RR184 pKa = 11.84QKK186 pKa = 8.81MPRR189 pKa = 11.84RR190 pKa = 11.84EE191 pKa = 4.08RR192 pKa = 11.84QPHH195 pKa = 6.74PIAVHH200 pKa = 6.31KK201 pKa = 10.4LAAQIARR208 pKa = 11.84DD209 pKa = 3.45WVGLKK214 pKa = 9.97RR215 pKa = 11.84CHH217 pKa = 6.59TGQICNAIVNAGIDD231 pKa = 3.7PGEE234 pKa = 3.84WTYY237 pKa = 11.98ARR239 pKa = 11.84LKK241 pKa = 10.69RR242 pKa = 11.84AVEE245 pKa = 3.94DD246 pKa = 3.68DD247 pKa = 3.34MRR249 pKa = 11.84ATGAVWPDD257 pKa = 3.16QVAYY261 pKa = 8.92PQGFLYY267 pKa = 10.78SRR269 pKa = 11.84LRR271 pKa = 11.84RR272 pKa = 11.84VSSQQ276 pKa = 2.7

Molecular weight: 30.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

16621

23

814

164.6

18.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.783 ± 0.377

0.884 ± 0.118

6.744 ± 0.203

6.696 ± 0.294

3.453 ± 0.183

8.243 ± 0.361

1.997 ± 0.144

5.138 ± 0.252

4.891 ± 0.217

7.912 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.419 ± 0.133

3.411 ± 0.174

5.162 ± 0.223

3.333 ± 0.178

6.311 ± 0.342

5.409 ± 0.206

6.119 ± 0.254

7.081 ± 0.245

2.148 ± 0.109

2.864 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski