candidate division Zixibacteria bacterium RBG-1

Taxonomy: cellular organisms; Bacteria; FCB group; candidate division Zixibacteria; unclassified candidate division Zixibacteria

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1906 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0MRB1|T0MRB1_9BACT Uncharacterized protein OS=candidate division Zixibacteria bacterium RBG-1 OX=1379698 GN=RBG1_1C00001G1274 PE=4 SV=1
MM1 pKa = 7.42GRR3 pKa = 11.84LDD5 pKa = 3.51LSLRR9 pKa = 11.84HH10 pKa = 6.16PPSLTSGIPQGDD22 pKa = 3.53NVNLLKK28 pKa = 10.82RR29 pKa = 11.84GVKK32 pKa = 9.64KK33 pKa = 10.61LKK35 pKa = 9.87KK36 pKa = 9.33SAMGLVVILIFLLASFQIAFSQNPDD61 pKa = 3.91SIPFAPAVDD70 pKa = 3.7YY71 pKa = 11.21AVGNNPDD78 pKa = 4.92DD79 pKa = 4.39NPDD82 pKa = 4.17CIFVSDD88 pKa = 4.82LDD90 pKa = 4.04GDD92 pKa = 4.11GDD94 pKa = 3.76IDD96 pKa = 4.72LAVAMSWNDD105 pKa = 3.63SISILMNNGDD115 pKa = 4.07GTFATQISYY124 pKa = 8.77PAGSYY129 pKa = 9.09PRR131 pKa = 11.84EE132 pKa = 4.05VFGSDD137 pKa = 3.25FDD139 pKa = 5.24SDD141 pKa = 3.7GDD143 pKa = 3.65TDD145 pKa = 4.2LAIANWYY152 pKa = 8.91GNSVSILKK160 pKa = 10.62NNGDD164 pKa = 3.49GTFYY168 pKa = 11.5AKK170 pKa = 10.16VDD172 pKa = 3.66YY173 pKa = 10.46DD174 pKa = 3.72AGVAPVSVFASDD186 pKa = 4.92LDD188 pKa = 3.81FDD190 pKa = 4.8GNQDD194 pKa = 3.61FVVSKK199 pKa = 10.23QSEE202 pKa = 4.24VGVFMNKK209 pKa = 9.08GDD211 pKa = 3.57ATFEE215 pKa = 4.02PRR217 pKa = 11.84VEE219 pKa = 4.19YY220 pKa = 9.36PAGGVSVYY228 pKa = 10.53ISDD231 pKa = 4.67LDD233 pKa = 3.94GDD235 pKa = 4.33GDD237 pKa = 3.99SDD239 pKa = 4.7LAVSSTGSDD248 pKa = 3.34EE249 pKa = 5.01ISILRR254 pKa = 11.84NNGDD258 pKa = 3.22GTFFPKK264 pKa = 10.34VNYY267 pKa = 8.42LTGHH271 pKa = 5.72VPTSIFVCDD280 pKa = 3.64IDD282 pKa = 5.74GDD284 pKa = 3.99TDD286 pKa = 3.28QDD288 pKa = 3.63IIVPNFSASTISVLKK303 pKa = 10.91NNGDD307 pKa = 3.46GTFATKK313 pKa = 10.27VDD315 pKa = 3.87YY316 pKa = 8.66PTEE319 pKa = 3.97YY320 pKa = 10.67SAVKK324 pKa = 10.22IIAIDD329 pKa = 3.8LDD331 pKa = 4.02LDD333 pKa = 3.89GDD335 pKa = 3.61QDD337 pKa = 4.23IIVSHH342 pKa = 6.54EE343 pKa = 4.14NNPIISIFKK352 pKa = 10.39NNGDD356 pKa = 3.42GTFPSRR362 pKa = 11.84LNYY365 pKa = 10.23SVGAGGVTHH374 pKa = 6.71WVVAADD380 pKa = 4.02LDD382 pKa = 4.35GDD384 pKa = 4.01GDD386 pKa = 4.21YY387 pKa = 11.67DD388 pKa = 3.93LAAANYY394 pKa = 8.25YY395 pKa = 10.49NSTVSVLMNLSPVCPLGYY413 pKa = 8.8STIQSLSSKK422 pKa = 10.3NINEE426 pKa = 4.07NEE428 pKa = 4.04SLTFKK433 pKa = 10.84VSVVKK438 pKa = 10.74GCTQIPVLSALNLPPNSSFFDD459 pKa = 3.49SGNGSGVFTFTPSYY473 pKa = 8.19TQSGTYY479 pKa = 9.88YY480 pKa = 9.56VTFTACDD487 pKa = 3.38TVACDD492 pKa = 3.85SEE494 pKa = 5.01TVQITVNCGTTKK506 pKa = 10.65RR507 pKa = 11.84GDD509 pKa = 3.61PNGNGQITLPDD520 pKa = 3.22VVYY523 pKa = 10.59LVNYY527 pKa = 7.69VFKK530 pKa = 10.75GGPAPVPEE538 pKa = 4.96KK539 pKa = 10.92CDD541 pKa = 3.14GDD543 pKa = 4.18ANNDD547 pKa = 3.62NQVGLIDD554 pKa = 4.93IIYY557 pKa = 8.87LANYY561 pKa = 8.62IFKK564 pKa = 10.3DD565 pKa = 3.73SPPPQPATCCLL576 pKa = 3.45

Molecular weight: 61.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0LED2|T0LED2_9BACT Purine nucleoside phosphorylase OS=candidate division Zixibacteria bacterium RBG-1 OX=1379698 GN=RBG1_1C00001G0102 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.98QPHH8 pKa = 4.95NRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84IKK14 pKa = 10.66DD15 pKa = 2.99HH16 pKa = 6.24GFRR19 pKa = 11.84KK20 pKa = 9.8RR21 pKa = 11.84MKK23 pKa = 8.65TKK25 pKa = 10.23GGRR28 pKa = 11.84KK29 pKa = 7.58VLRR32 pKa = 11.84KK33 pKa = 9.02RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.15GRR39 pKa = 11.84HH40 pKa = 5.44RR41 pKa = 11.84LTVTVPKK48 pKa = 10.72AA49 pKa = 3.08

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1906

0

1906

641823

29

5949

336.7

37.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.815 ± 0.05

0.954 ± 0.028

4.93 ± 0.048

6.452 ± 0.078

4.947 ± 0.051

7.099 ± 0.063

1.55 ± 0.024

7.301 ± 0.061

8.082 ± 0.099

10.486 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.897 ± 0.032

4.595 ± 0.058

4.211 ± 0.037

3.559 ± 0.038

3.992 ± 0.038

6.435 ± 0.056

5.193 ± 0.073

6.861 ± 0.051

1.17 ± 0.021

3.473 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski