Murine pneumonia virus (strain 15) (MPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Pneumoviridae; Orthopneumovirus; Murine orthopneumovirus

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5MKM7|PHOSP_MPV15 Phosphoprotein OS=Murine pneumonia virus (strain 15) OX=296738 GN=P PE=3 SV=1
MM1 pKa = 7.59EE2 pKa = 4.81KK3 pKa = 10.16FAPEE7 pKa = 4.1FVGEE11 pKa = 4.24DD12 pKa = 3.32ANKK15 pKa = 10.21KK16 pKa = 10.09AEE18 pKa = 4.2EE19 pKa = 4.03FLKK22 pKa = 10.78HH23 pKa = 6.57RR24 pKa = 11.84SFPSEE29 pKa = 4.14KK30 pKa = 9.72PLAGIPNTATHH41 pKa = 4.6VTKK44 pKa = 11.22YY45 pKa = 10.91NMPPILRR52 pKa = 11.84SSFKK56 pKa = 10.82LPSPRR61 pKa = 11.84VAANLTEE68 pKa = 4.54PSAPPTTPPPTPPQNKK84 pKa = 9.02EE85 pKa = 4.0EE86 pKa = 4.08QPKK89 pKa = 10.61EE90 pKa = 3.97SDD92 pKa = 3.06VDD94 pKa = 3.37IEE96 pKa = 4.4TMHH99 pKa = 5.92VCKK102 pKa = 10.97VPDD105 pKa = 3.65NPEE108 pKa = 3.91HH109 pKa = 6.81SKK111 pKa = 10.94KK112 pKa = 10.2PCCSDD117 pKa = 3.33DD118 pKa = 3.54TDD120 pKa = 3.73TKK122 pKa = 10.05KK123 pKa = 8.88TRR125 pKa = 11.84KK126 pKa = 9.57PMVTFVEE133 pKa = 4.45PEE135 pKa = 3.94EE136 pKa = 4.52KK137 pKa = 10.32FVGLGASLYY146 pKa = 10.21RR147 pKa = 11.84EE148 pKa = 4.25TMQTFAADD156 pKa = 4.28GYY158 pKa = 10.96DD159 pKa = 3.57EE160 pKa = 4.49EE161 pKa = 5.77SNLSFEE167 pKa = 4.68EE168 pKa = 4.5TNQEE172 pKa = 4.04PGSSSVEE179 pKa = 3.72QRR181 pKa = 11.84LDD183 pKa = 3.74RR184 pKa = 11.84IEE186 pKa = 4.7EE187 pKa = 4.01KK188 pKa = 10.6LSYY191 pKa = 10.39IIGLLNTIMVATAGPTTARR210 pKa = 11.84DD211 pKa = 3.91EE212 pKa = 4.32IRR214 pKa = 11.84DD215 pKa = 3.73ALIGTRR221 pKa = 11.84EE222 pKa = 4.02EE223 pKa = 4.52LIEE226 pKa = 4.22MIKK229 pKa = 10.57SDD231 pKa = 3.56ILTVNDD237 pKa = 4.83RR238 pKa = 11.84IVAMEE243 pKa = 3.95KK244 pKa = 10.61LRR246 pKa = 11.84DD247 pKa = 3.97EE248 pKa = 4.61EE249 pKa = 4.44CSRR252 pKa = 11.84ADD254 pKa = 3.4TDD256 pKa = 5.52DD257 pKa = 4.83GSACYY262 pKa = 8.95LTDD265 pKa = 3.61RR266 pKa = 11.84ARR268 pKa = 11.84ILDD271 pKa = 4.28KK272 pKa = 10.67IVSSNAEE279 pKa = 3.88EE280 pKa = 4.12AKK282 pKa = 10.64EE283 pKa = 4.12DD284 pKa = 4.47LDD286 pKa = 3.84VDD288 pKa = 4.74DD289 pKa = 5.61IMGINFF295 pKa = 3.75

Molecular weight: 32.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q5MKM7|PHOSP_MPV15 Phosphoprotein OS=Murine pneumonia virus (strain 15) OX=296738 GN=P PE=3 SV=1
MM1 pKa = 7.5SPNITCPLYY10 pKa = 10.92CVAHH14 pKa = 6.3SNSLPRR20 pKa = 11.84EE21 pKa = 3.91LLQILLNPLLPLPLHH36 pKa = 6.52HH37 pKa = 7.06PHH39 pKa = 7.28LPRR42 pKa = 11.84TRR44 pKa = 11.84KK45 pKa = 9.7SSPKK49 pKa = 10.59SLMLTLRR56 pKa = 11.84LCMSVRR62 pKa = 11.84FLTIRR67 pKa = 11.84NTARR71 pKa = 11.84SHH73 pKa = 5.98AAQMIPILRR82 pKa = 11.84KK83 pKa = 9.87LGSRR87 pKa = 11.84WSPLWNPRR95 pKa = 11.84RR96 pKa = 11.84NLSDD100 pKa = 3.3WEE102 pKa = 4.26LACTGRR108 pKa = 11.84PCRR111 pKa = 11.84PLLLMVMMKK120 pKa = 10.2KK121 pKa = 9.03ATYY124 pKa = 10.09RR125 pKa = 11.84LRR127 pKa = 11.84RR128 pKa = 11.84LTKK131 pKa = 10.42SRR133 pKa = 11.84VLHH136 pKa = 6.18LL137 pKa = 4.29

Molecular weight: 15.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

4657

92

2040

388.1

43.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.239 ± 0.933

2.319 ± 0.365

5.132 ± 0.366

5.068 ± 0.556

3.457 ± 0.395

4.853 ± 0.411

2.491 ± 0.265

6.764 ± 0.516

6.141 ± 0.358

10.994 ± 0.878

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.196

5.841 ± 0.307

4.531 ± 0.753

3.028 ± 0.301

5.175 ± 0.413

8.267 ± 0.394

6.657 ± 0.691

6.335 ± 0.561

1.16 ± 0.243

3.758 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski