Gordonia phage Yeezy
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A142K9I0|A0A142K9I0_9CAUD Tape measure protein OS=Gordonia phage Yeezy OX=1821565 GN=18 PE=4 SV=1
MM1 pKa = 7.57 KK2 pKa = 9.85 MRR4 pKa = 11.84 MIGAAIGVPAALLIGGCSDD23 pKa = 3.58 DD24 pKa = 3.93 EE25 pKa = 4.47 SWRR28 pKa = 11.84 TTAAEE33 pKa = 4.58 GACEE37 pKa = 4.18 DD38 pKa = 4.22 SVKK41 pKa = 10.96 AQLKK45 pKa = 10.69 DD46 pKa = 3.38 PDD48 pKa = 3.91 SADD51 pKa = 3.38 FDD53 pKa = 4.18 GVDD56 pKa = 3.83 VVDD59 pKa = 5.07 NGDD62 pKa = 3.13 GTYY65 pKa = 10.88 SVTGRR70 pKa = 11.84 VNAEE74 pKa = 3.6 NSFGGMTGFQDD85 pKa = 4.07 FEE87 pKa = 4.27 CAARR91 pKa = 11.84 DD92 pKa = 3.79 DD93 pKa = 4.43 GDD95 pKa = 3.92 NVTGRR100 pKa = 11.84 ATLLGG105 pKa = 3.95
Molecular weight: 10.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.25
IPC2_protein 3.884
IPC_protein 3.872
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.821
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.846
Sillero 3.999
Patrickios 3.719
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.923
Protein with the highest isoelectric point:
>tr|A0A1L7JNR9|A0A1L7JNR9_9CAUD Uncharacterized protein OS=Gordonia phage Yeezy OX=1821565 GN=13 PE=4 SV=1
MM1 pKa = 7.25 PTAKK5 pKa = 9.64 QRR7 pKa = 11.84 NTTQRR12 pKa = 11.84 GLGWSHH18 pKa = 5.24 QKK20 pKa = 9.88 QRR22 pKa = 11.84 VRR24 pKa = 11.84 LLGKK28 pKa = 8.53 HH29 pKa = 6.03 TDD31 pKa = 3.51 TTPCWWCGEE40 pKa = 3.85 PMYY43 pKa = 10.47 RR44 pKa = 11.84 DD45 pKa = 3.77 PKK47 pKa = 10.58 RR48 pKa = 11.84 NPDD51 pKa = 3.58 YY52 pKa = 11.28 DD53 pKa = 3.95 PASNDD58 pKa = 3.24 PDD60 pKa = 3.99 TGKK63 pKa = 10.4 LAADD67 pKa = 3.8 HH68 pKa = 5.9 TRR70 pKa = 11.84 SRR72 pKa = 11.84 ATGGTVADD80 pKa = 4.41 RR81 pKa = 11.84 LLHH84 pKa = 5.56 GLCNKK89 pKa = 9.44 QRR91 pKa = 11.84 GDD93 pKa = 3.34 GSRR96 pKa = 11.84 DD97 pKa = 3.25 HH98 pKa = 6.57 QRR100 pKa = 11.84 PALLKK105 pKa = 10.54 RR106 pKa = 11.84 RR107 pKa = 11.84 GGHH110 pKa = 6.49 PANSLNWGG118 pKa = 3.56
Molecular weight: 13.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.309
IPC2_protein 9.414
IPC_protein 9.78
Toseland 10.526
ProMoST 10.101
Dawson 10.613
Bjellqvist 10.277
Wikipedia 10.774
Rodwell 10.906
Grimsley 10.643
Solomon 10.701
Lehninger 10.672
Nozaki 10.526
DTASelect 10.262
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.54
Patrickios 10.672
IPC_peptide 10.701
IPC2_peptide 9.311
IPC2.peptide.svr19 8.489
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
86
0
86
16833
28
2109
195.7
21.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.036 ± 0.625
0.861 ± 0.16
6.998 ± 0.377
5.953 ± 0.307
2.513 ± 0.154
8.204 ± 0.332
2.186 ± 0.23
4.242 ± 0.153
3.374 ± 0.234
7.664 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.044 ± 0.162
2.994 ± 0.196
5.673 ± 0.305
3.6 ± 0.268
7.628 ± 0.419
5.513 ± 0.198
7.39 ± 0.314
7.04 ± 0.238
2.02 ± 0.195
2.067 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here