Paenibacillus phage Vadim

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gochnauervirinae; Vegasvirus; Paenibacillus virus Vegas

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2CZ86|A0A0K2CZ86_9CAUD AbrB family transcriptional regulator OS=Paenibacillus phage Vadim OX=1636260 GN=VADIM_64 PE=4 SV=1
MM1 pKa = 7.62ISQNGRR7 pKa = 11.84EE8 pKa = 4.65GKK10 pKa = 10.6EE11 pKa = 4.06MICPFCGDD19 pKa = 4.37EE20 pKa = 4.13MEE22 pKa = 4.88HH23 pKa = 6.21WNQGIYY29 pKa = 9.14EE30 pKa = 4.34CKK32 pKa = 9.97DD33 pKa = 3.78CEE35 pKa = 4.77VMIPDD40 pKa = 6.22DD41 pKa = 4.55IDD43 pKa = 5.09DD44 pKa = 5.14DD45 pKa = 4.35YY46 pKa = 12.16ASEE49 pKa = 4.01II50 pKa = 4.11

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2CZH1|A0A0K2CZH1_9CAUD AAA domain ATPase OS=Paenibacillus phage Vadim OX=1636260 GN=VADIM_68 PE=4 SV=1
MM1 pKa = 7.4ARR3 pKa = 11.84IDD5 pKa = 4.93KK6 pKa = 10.26IVQKK10 pKa = 10.24MKK12 pKa = 10.44NRR14 pKa = 11.84PNGVRR19 pKa = 11.84LEE21 pKa = 4.47EE22 pKa = 4.15IMTVLNHH29 pKa = 6.39NGYY32 pKa = 9.76ILVRR36 pKa = 11.84TKK38 pKa = 10.77GSHH41 pKa = 3.8HH42 pKa = 6.85HH43 pKa = 6.16FRR45 pKa = 11.84NEE47 pKa = 3.69QGDD50 pKa = 5.21LITLPRR56 pKa = 11.84QNPLKK61 pKa = 10.18AAYY64 pKa = 9.92VRR66 pKa = 11.84DD67 pKa = 3.51VLKK70 pKa = 10.88RR71 pKa = 11.84IGKK74 pKa = 9.37

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

14471

29

1234

168.3

19.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.897 ± 0.471

0.961 ± 0.128

5.632 ± 0.277

8.292 ± 0.38

3.856 ± 0.235

6.461 ± 0.256

1.914 ± 0.187

6.489 ± 0.319

7.919 ± 0.283

8.603 ± 0.204

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.027 ± 0.152

4.575 ± 0.194

3.559 ± 0.158

4.367 ± 0.163

5.252 ± 0.386

5.825 ± 0.196

4.941 ± 0.194

6.53 ± 0.295

1.486 ± 0.148

3.407 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski