Torque teno felis virus 2
Average proteome isoelectric point is 7.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8DMP7|A8DMP7_9VIRU Capsid protein OS=Torque teno felis virus 2 OX=2065043 PE=3 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.69 RR3 pKa = 11.84 PLPGPNVMLPAISLTDD19 pKa = 3.88 PPNLDD24 pKa = 2.46 HH25 pKa = 7.03 HH26 pKa = 6.4 LAYY29 pKa = 10.06 RR30 pKa = 11.84 RR31 pKa = 11.84 KK32 pKa = 8.88 EE33 pKa = 4.34 AIWKK37 pKa = 8.74 QLISSEE43 pKa = 4.16 HH44 pKa = 6.5 KK45 pKa = 9.87 KK46 pKa = 9.29 WCLCGSYY53 pKa = 9.98 RR54 pKa = 11.84 NHH56 pKa = 6.85 FLPPEE61 pKa = 4.18 KK62 pKa = 10.74 SLINEE67 pKa = 4.22 EE68 pKa = 4.51 KK69 pKa = 10.32 SCSEE73 pKa = 4.1 DD74 pKa = 3.48 VVTGGGGDD82 pKa = 3.53 GRR84 pKa = 11.84 EE85 pKa = 4.38 GEE87 pKa = 4.79 DD88 pKa = 4.22 DD89 pKa = 3.83 SKK91 pKa = 12.25 DD92 pKa = 3.62 MVGEE96 pKa = 4.04 DD97 pKa = 4.23 AISDD101 pKa = 3.91 AEE103 pKa = 4.15 LL104 pKa = 4.17
Molecular weight: 11.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.836
IPC2_protein 4.863
IPC_protein 4.762
Toseland 4.647
ProMoST 4.876
Dawson 4.724
Bjellqvist 4.863
Wikipedia 4.596
Rodwell 4.635
Grimsley 4.571
Solomon 4.724
Lehninger 4.673
Nozaki 4.838
DTASelect 4.991
Thurlkill 4.66
EMBOSS 4.622
Sillero 4.902
Patrickios 3.986
IPC_peptide 4.736
IPC2_peptide 4.902
IPC2.peptide.svr19 4.859
Protein with the highest isoelectric point:
>tr|A8DMP8|A8DMP8_9VIRU ORF3 OS=Torque teno felis virus 2 OX=2065043 PE=4 SV=1
MM1 pKa = 7.17 FRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 YY7 pKa = 9.26 RR8 pKa = 11.84 RR9 pKa = 11.84 WRR11 pKa = 11.84 RR12 pKa = 11.84 WPRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 FKK21 pKa = 8.8 RR22 pKa = 11.84 HH23 pKa = 3.21 GWRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 HH29 pKa = 4.59 FRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 AVSRR37 pKa = 11.84 QTVHH41 pKa = 4.79 EE42 pKa = 4.87 WIPGRR47 pKa = 11.84 HH48 pKa = 4.46 RR49 pKa = 11.84 VVTVRR54 pKa = 11.84 GWEE57 pKa = 3.91 PLGNLCSTDD66 pKa = 3.3 TPKK69 pKa = 10.59 QEE71 pKa = 3.64 ASPYY75 pKa = 10.3 HH76 pKa = 6.4 SVEE79 pKa = 4.04 PQTATGQWHH88 pKa = 5.3 GTWGKK93 pKa = 10.26 HH94 pKa = 4.65 YY95 pKa = 8.96 FTVQNLLLRR104 pKa = 11.84 AQARR108 pKa = 11.84 WNQWSDD114 pKa = 2.45 DD115 pKa = 3.45 WTTYY119 pKa = 11.06 DD120 pKa = 3.77 YY121 pKa = 11.45 IKK123 pKa = 10.18 FTGGAILIPPPSSTQFMINFDD144 pKa = 4.54 PYY146 pKa = 11.2 LQTTVLLYY154 pKa = 9.52 QEE156 pKa = 5.27 KK157 pKa = 10.1 NAEE160 pKa = 4.23 DD161 pKa = 4.68 KK162 pKa = 11.0 YY163 pKa = 9.54 IHH165 pKa = 6.82 PGILMHH171 pKa = 7.32 DD172 pKa = 4.36 PKK174 pKa = 10.53 THH176 pKa = 6.98 IIFAANYY183 pKa = 6.61 MPRR186 pKa = 11.84 KK187 pKa = 9.34 RR188 pKa = 11.84 MYY190 pKa = 10.11 KK191 pKa = 9.68 IRR193 pKa = 11.84 IKK195 pKa = 10.46 PPPGWKK201 pKa = 9.97 GYY203 pKa = 7.9 QRR205 pKa = 11.84 FPDD208 pKa = 3.28 AMGYY212 pKa = 9.78 ILTHH216 pKa = 6.16 WAWTWFSMNQAFFTTTGQGSVCAAEE241 pKa = 4.63 PWWSGNNYY249 pKa = 7.84 LQKK252 pKa = 10.21 WVDD255 pKa = 3.05 RR256 pKa = 11.84 SKK258 pKa = 11.73 YY259 pKa = 9.6 EE260 pKa = 5.0 DD261 pKa = 3.2 ISASTGRR268 pKa = 11.84 NWGPFLPAKK277 pKa = 9.57 YY278 pKa = 10.28 GNYY281 pKa = 9.73 PEE283 pKa = 4.33 TSLFFKK289 pKa = 10.98 YY290 pKa = 10.44 KK291 pKa = 10.76 LFFKK295 pKa = 10.93 LVGNAIWRR303 pKa = 11.84 PLPRR307 pKa = 11.84 NYY309 pKa = 10.73 LKK311 pKa = 10.89 DD312 pKa = 3.56 GLVDD316 pKa = 3.64 QPEE319 pKa = 4.6 GPTAGGEE326 pKa = 4.16 ADD328 pKa = 4.16 SPTAHH333 pKa = 6.64 KK334 pKa = 10.2 KK335 pKa = 8.42 ARR337 pKa = 11.84 KK338 pKa = 9.25 RR339 pKa = 11.84 ISRR342 pKa = 11.84 PTSEE346 pKa = 3.73 ADD348 pKa = 3.06 IRR350 pKa = 11.84 HH351 pKa = 6.28 SDD353 pKa = 3.68 LDD355 pKa = 3.53 SDD357 pKa = 5.61 GILTSPAYY365 pKa = 10.22 RR366 pKa = 11.84 RR367 pKa = 11.84 ITYY370 pKa = 9.62 DD371 pKa = 2.59 HH372 pKa = 6.79 SRR374 pKa = 11.84 PKK376 pKa = 10.11 RR377 pKa = 11.84 RR378 pKa = 11.84 KK379 pKa = 10.12 LEE381 pKa = 4.08 DD382 pKa = 3.45 LQRR385 pKa = 11.84 LGSLYY390 pKa = 10.94 DD391 pKa = 3.64 RR392 pKa = 11.84 VQHH395 pKa = 6.32 VLAEE399 pKa = 4.24 FNLLKK404 pKa = 10.85
Molecular weight: 48.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.823
IPC_protein 10.526
Toseland 10.467
ProMoST 10.218
Dawson 10.643
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.891
Grimsley 10.716
Solomon 10.701
Lehninger 10.657
Nozaki 10.438
DTASelect 10.35
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.555
Patrickios 10.511
IPC_peptide 10.701
IPC2_peptide 9.209
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
691
104
404
230.3
26.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.065 ± 0.786
0.868 ± 0.556
5.065 ± 1.051
5.065 ± 1.682
3.618 ± 0.84
6.078 ± 0.98
2.894 ± 0.93
4.631 ± 0.148
6.223 ± 0.239
8.828 ± 1.678
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.605 ± 0.78
3.184 ± 0.396
8.104 ± 0.927
4.052 ± 0.887
8.249 ± 2.422
8.104 ± 2.412
6.657 ± 1.432
3.618 ± 0.128
3.329 ± 1.159
3.763 ± 1.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here