Snake adenovirus serotype 1 (SnAdV-1)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|A9CBA0|ORF1_ADES1 Protein ORF1 OS=Snake adenovirus serotype 1 OX=189830 PE=4 SV=1
MM1 pKa = 7.8 GDD3 pKa = 4.13 NYY5 pKa = 10.56 WIDD8 pKa = 3.83 PVNLSPQEE16 pKa = 3.92 LVEE19 pKa = 3.95 TLQRR23 pKa = 11.84 IDD25 pKa = 3.32 QEE27 pKa = 4.61 GYY29 pKa = 8.5 EE30 pKa = 4.33 VIVVTEE36 pKa = 4.37 GGFPMGPFRR45 pKa = 11.84 MAFRR49 pKa = 11.84 IDD51 pKa = 3.85 EE52 pKa = 4.66 EE53 pKa = 4.47 PNMYY57 pKa = 10.52 YY58 pKa = 10.0 ISQKK62 pKa = 11.04 DD63 pKa = 3.32 LALEE67 pKa = 3.85 YY68 pKa = 11.06 DD69 pKa = 4.17 PLQFLHH75 pKa = 6.88 WVRR78 pKa = 11.84 QYY80 pKa = 11.18 SGEE83 pKa = 4.11 IINWTHH89 pKa = 7.0 HH90 pKa = 5.9 GRR92 pKa = 11.84 VPWWKK97 pKa = 9.49 IFQMLEE103 pKa = 3.66 AKK105 pKa = 10.77 LEE107 pKa = 3.98 DD108 pKa = 3.25 SYY110 pKa = 12.07 YY111 pKa = 10.79 YY112 pKa = 8.1 EE113 pKa = 3.79 TTMFYY118 pKa = 10.01 IPSSISHH125 pKa = 6.8 DD126 pKa = 3.54 CWSLFLQFWRR136 pKa = 11.84 EE137 pKa = 4.15 GFGSPPLDD145 pKa = 3.74 VAWSS149 pKa = 3.54
Molecular weight: 17.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.171
IPC2_protein 4.444
IPC_protein 4.355
Toseland 4.202
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.177
Rodwell 4.202
Grimsley 4.113
Solomon 4.304
Lehninger 4.253
Nozaki 4.418
DTASelect 4.558
Thurlkill 4.215
EMBOSS 4.19
Sillero 4.469
Patrickios 1.138
IPC_peptide 4.304
IPC2_peptide 4.457
IPC2.peptide.svr19 4.379
Protein with the highest isoelectric point:
>sp|Q8JN70|NP_ADES1 Pre-histone-like nucleoprotein OS=Snake adenovirus serotype 1 OX=189830 GN=L2 PE=3 SV=1
MM1 pKa = 7.58 ARR3 pKa = 11.84 LRR5 pKa = 11.84 RR6 pKa = 11.84 AKK8 pKa = 8.8 RR9 pKa = 11.84 TSYY12 pKa = 10.0 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 TTRR20 pKa = 11.84 TRR22 pKa = 11.84 TVVTRR27 pKa = 11.84 TARR30 pKa = 11.84 STVRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 GGRR39 pKa = 11.84 IVYY42 pKa = 7.63 RR43 pKa = 11.84 TSKK46 pKa = 5.57 TTRR49 pKa = 11.84 VGTRR53 pKa = 11.84 RR54 pKa = 11.84 MRR56 pKa = 11.84 GGFLPLLAPILAAAIGSIPGIVIAAKK82 pKa = 10.25 QKK84 pKa = 10.52
Molecular weight: 9.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.48
IPC2_protein 11.286
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.384
Grimsley 12.852
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.106
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
28
0
28
9074
58
1085
324.1
36.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.877 ± 0.335
1.995 ± 0.401
4.805 ± 0.251
5.852 ± 0.421
4.386 ± 0.258
6.436 ± 0.418
1.907 ± 0.202
3.989 ± 0.187
4.474 ± 0.597
9.555 ± 0.366
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.292 ± 0.128
4.761 ± 0.521
6.612 ± 0.355
4.397 ± 0.301
6.767 ± 0.66
7.836 ± 0.509
5.709 ± 0.416
6.436 ± 0.274
1.411 ± 0.131
3.505 ± 0.297
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here