Salem virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Salemvirus; Salem salemvirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9IZC0|Q9IZC0_9MONO Isoform of I6UID6 V protein OS=Salem virus OX=120499 GN=C PE=4 SV=1
MM1 pKa = 7.5SDD3 pKa = 3.4EE4 pKa = 4.21NRR6 pKa = 11.84TLVKK10 pKa = 10.65NALKK14 pKa = 10.84VLADD18 pKa = 4.42LKK20 pKa = 10.67KK21 pKa = 10.6QEE23 pKa = 4.38NKK25 pKa = 10.34PIGEE29 pKa = 4.03PRR31 pKa = 11.84ITLQNLRR38 pKa = 11.84EE39 pKa = 4.24HH40 pKa = 6.41YY41 pKa = 10.45GKK43 pKa = 10.17NDD45 pKa = 3.26YY46 pKa = 11.61AKK48 pKa = 10.54FGEE51 pKa = 4.54DD52 pKa = 3.2LQEE55 pKa = 4.02VHH57 pKa = 6.98LSLQKK62 pKa = 10.48RR63 pKa = 11.84PQCNVEE69 pKa = 3.71LRR71 pKa = 11.84QPGGDD76 pKa = 3.24QDD78 pKa = 3.39NSPRR82 pKa = 11.84IKK84 pKa = 9.95TGNQEE89 pKa = 3.72GNLQVDD95 pKa = 4.15TEE97 pKa = 4.68TTGEE101 pKa = 4.26VSCTAGSSCGDD112 pKa = 3.18SDD114 pKa = 4.22SPHH117 pKa = 7.08DD118 pKa = 4.06AEE120 pKa = 4.18QASRR124 pKa = 11.84RR125 pKa = 11.84GVADD129 pKa = 3.62AGLAAFCTEE138 pKa = 4.24GNSGFSNHH146 pKa = 5.59MRR148 pKa = 11.84GHH150 pKa = 6.3GPVSGDD156 pKa = 3.78DD157 pKa = 3.74FGLVPAKK164 pKa = 10.46VPRR167 pKa = 11.84SSARR171 pKa = 11.84DD172 pKa = 3.4PTYY175 pKa = 10.8SSDD178 pKa = 3.28VRR180 pKa = 11.84GVSKK184 pKa = 11.01LDD186 pKa = 3.7DD187 pKa = 4.02FADD190 pKa = 3.38ILLTVGASEE199 pKa = 4.39FSFDD203 pKa = 4.8HH204 pKa = 6.29IVSTAQADD212 pKa = 4.16LEE214 pKa = 4.53EE215 pKa = 4.66SLSLQPAPINEE226 pKa = 4.24EE227 pKa = 4.1ALPSTSTGAPEE238 pKa = 5.03NGDD241 pKa = 3.27KK242 pKa = 10.95QEE244 pKa = 4.52AIKK247 pKa = 10.6KK248 pKa = 8.67GSRR251 pKa = 11.84HH252 pKa = 4.74RR253 pKa = 11.84RR254 pKa = 11.84EE255 pKa = 3.84YY256 pKa = 10.87SIIWDD261 pKa = 3.59SEE263 pKa = 4.41GIQIEE268 pKa = 4.7SWCNPVCSKK277 pKa = 10.35VRR279 pKa = 11.84STPRR283 pKa = 11.84RR284 pKa = 11.84EE285 pKa = 3.59KK286 pKa = 10.57CRR288 pKa = 11.84CGKK291 pKa = 10.35CPARR295 pKa = 11.84CSEE298 pKa = 4.67CGDD301 pKa = 3.92DD302 pKa = 4.41

Molecular weight: 32.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9IZC0|Q9IZC0_9MONO Isoform of I6UID6 V protein OS=Salem virus OX=120499 GN=C PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 9.96IEE4 pKa = 4.34HH5 pKa = 7.26LSRR8 pKa = 11.84MLSRR12 pKa = 11.84FLQILKK18 pKa = 10.18SKK20 pKa = 10.53RR21 pKa = 11.84INPLEE26 pKa = 4.11NQEE29 pKa = 3.87SHH31 pKa = 6.03YY32 pKa = 9.93RR33 pKa = 11.84TLEE36 pKa = 4.22SITEE40 pKa = 4.14KK41 pKa = 8.45TTMPNSGKK49 pKa = 8.29TSKK52 pKa = 10.73KK53 pKa = 10.4SIYY56 pKa = 9.71LCKK59 pKa = 9.63RR60 pKa = 11.84DD61 pKa = 3.8PSAMSNSASRR71 pKa = 11.84VEE73 pKa = 4.43IKK75 pKa = 9.94TIAHH79 pKa = 6.55EE80 pKa = 3.91LRR82 pKa = 11.84QEE84 pKa = 3.57IRR86 pKa = 11.84KK87 pKa = 9.45EE88 pKa = 3.51ISKK91 pKa = 9.66WIQKK95 pKa = 7.68QQVKK99 pKa = 10.38SVVPQDD105 pKa = 3.43LHH107 pKa = 8.99VEE109 pKa = 4.24TQILLTMLSRR119 pKa = 11.84LAEE122 pKa = 4.5GEE124 pKa = 4.99LLTQDD129 pKa = 3.1WQRR132 pKa = 11.84SVLKK136 pKa = 9.94EE137 pKa = 3.97IQDD140 pKa = 3.67SAITCEE146 pKa = 4.86VMDD149 pKa = 4.38QLVAMILDD157 pKa = 4.04LFPRR161 pKa = 11.84KK162 pKa = 9.71YY163 pKa = 9.93HH164 pKa = 6.5DD165 pKa = 3.62QVQGIQPIPVMLGVV179 pKa = 3.72

Molecular weight: 20.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

5116

179

2229

639.5

71.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.414 ± 0.396

1.603 ± 0.252

5.473 ± 0.44

5.512 ± 0.606

3.382 ± 0.402

5.864 ± 0.372

2.541 ± 0.25

7.115 ± 0.557

5.356 ± 0.529

11.474 ± 0.578

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.263

4.359 ± 0.28

4.789 ± 0.54

3.87 ± 0.348

5.395 ± 0.374

8.64 ± 0.493

6.04 ± 0.452

6.294 ± 0.297

1.075 ± 0.244

3.616 ± 0.544
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski