Epulopiscium sp. SCG-B11WGA-EpuloA1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Epulopiscium; unclassified Epulopiscium

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2M2K7|A0A1V2M2K7_9FIRM Damage-inducible protein CinA OS=Epulopiscium sp. SCG-B11WGA-EpuloA1 OX=1710701 GN=AN396_02805 PE=3 SV=1
MM1 pKa = 7.54NYY3 pKa = 9.91IKK5 pKa = 10.85GDD7 pKa = 3.47DD8 pKa = 3.79TLYY11 pKa = 11.27LSIDD15 pKa = 3.52PFSSKK20 pKa = 10.56FEE22 pKa = 4.23SPDD25 pKa = 3.2NSKK28 pKa = 10.64LLEE31 pKa = 4.74TIDD34 pKa = 3.99DD35 pKa = 3.99TNVYY39 pKa = 10.38YY40 pKa = 10.77SEE42 pKa = 4.32TLFKK46 pKa = 10.63VVPEE50 pKa = 4.9GYY52 pKa = 10.33VLTPEE57 pKa = 4.36EE58 pKa = 4.66EE59 pKa = 4.23KK60 pKa = 10.78QQLAGKK66 pKa = 8.36LTISFGDD73 pKa = 3.12SDD75 pKa = 4.41GTVEE79 pKa = 4.97TYY81 pKa = 8.9QHH83 pKa = 6.04MSWTEE88 pKa = 3.63DD89 pKa = 3.04GNLYY93 pKa = 10.56SLSGFNCDD101 pKa = 3.69LSASEE106 pKa = 4.37MLSMAEE112 pKa = 4.92DD113 pKa = 4.49IINEE117 pKa = 4.04

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2LY42|A0A1V2LY42_9FIRM Glyco_trans_2-like domain-containing protein OS=Epulopiscium sp. SCG-B11WGA-EpuloA1 OX=1710701 GN=AN396_09905 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 8.13MTLQPKK8 pKa = 8.85KK9 pKa = 8.37RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84EE15 pKa = 3.65HH16 pKa = 6.35GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.15NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.19GRR39 pKa = 11.84RR40 pKa = 11.84VLSAA44 pKa = 3.76

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2684

0

2684

897904

37

7255

334.5

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.091 ± 0.05

1.131 ± 0.021

5.584 ± 0.054

7.189 ± 0.059

4.323 ± 0.034

6.413 ± 0.045

1.512 ± 0.018

9.724 ± 0.059

7.642 ± 0.054

8.925 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.803 ± 0.026

6.084 ± 0.041

3.265 ± 0.034

3.172 ± 0.031

2.923 ± 0.032

6.028 ± 0.037

5.861 ± 0.061

6.429 ± 0.044

0.79 ± 0.017

4.111 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski