Beihai sipunculid worm virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHN2|A0A1L3KHN2_9VIRU Uncharacterized protein OS=Beihai sipunculid worm virus 5 OX=1922677 PE=4 SV=1
MM1 pKa = 7.63SMPATATGTPKK12 pKa = 10.76NYY14 pKa = 10.07DD15 pKa = 2.85VSAQGGGPAALDD27 pKa = 3.72TQQVPVEE34 pKa = 4.27ASQTAIVAHH43 pKa = 6.28SGDD46 pKa = 3.78VNVIDD51 pKa = 5.31PYY53 pKa = 10.8LKK55 pKa = 10.36KK56 pKa = 10.16HH57 pKa = 6.67FILNSIFRR65 pKa = 11.84WTTAMPAGTIIRR77 pKa = 11.84KK78 pKa = 9.2IPVHH82 pKa = 6.27PSYY85 pKa = 11.54ANPQVQHH92 pKa = 6.67LSQMYY97 pKa = 9.1NLWAGSLDD105 pKa = 3.77YY106 pKa = 10.66KK107 pKa = 10.81IEE109 pKa = 3.87IAGTGFHH116 pKa = 6.99GGRR119 pKa = 11.84LLVAKK124 pKa = 9.49VPPNLNPDD132 pKa = 3.68DD133 pKa = 4.16YY134 pKa = 10.32TPQGLTVFHH143 pKa = 6.26NVEE146 pKa = 4.07IEE148 pKa = 4.35VKK150 pKa = 10.13QSMGGTLTAIDD161 pKa = 3.65QKK163 pKa = 11.41NIVYY167 pKa = 9.89HH168 pKa = 5.71YY169 pKa = 10.73NSGSKK174 pKa = 10.29PEE176 pKa = 4.57LGSVDD181 pKa = 3.52GGTLVIIVLYY191 pKa = 9.42PLVLAAEE198 pKa = 4.52QNGSVNLGLYY208 pKa = 10.23SKK210 pKa = 10.91LGDD213 pKa = 3.77DD214 pKa = 4.42FVFSQMQPVLQTTTYY229 pKa = 11.22NPTHH233 pKa = 6.83LNNMFRR239 pKa = 11.84FDD241 pKa = 4.22HH242 pKa = 6.76RR243 pKa = 11.84EE244 pKa = 3.84CLHH247 pKa = 6.38PVVTACVFKK256 pKa = 10.9KK257 pKa = 10.65LRR259 pKa = 11.84VSKK262 pKa = 9.88NQKK265 pKa = 8.51EE266 pKa = 4.73VYY268 pKa = 9.87HH269 pKa = 6.21GLGFLAGADD278 pKa = 3.42DD279 pKa = 3.66TRR281 pKa = 11.84RR282 pKa = 11.84LFKK285 pKa = 9.88EE286 pKa = 3.69HH287 pKa = 7.14RR288 pKa = 11.84NDD290 pKa = 3.71TRR292 pKa = 11.84GCSNTDD298 pKa = 2.96GTSATPVQGFTRR310 pKa = 11.84IFEE313 pKa = 4.39SQVQFGLNIYY323 pKa = 10.04HH324 pKa = 6.69NGGNGVTDD332 pKa = 3.24QVYY335 pKa = 7.99TAQGNVSNPAKK346 pKa = 10.36GYY348 pKa = 9.63VWLQHH353 pKa = 6.56GIHH356 pKa = 7.16DD357 pKa = 4.34AVQNEE362 pKa = 3.93NAYY365 pKa = 10.04CAEE368 pKa = 4.2GTKK371 pKa = 10.18SHH373 pKa = 7.42LDD375 pKa = 3.74PIAWYY380 pKa = 9.18EE381 pKa = 4.66DD382 pKa = 4.08PLCPPPAPVSTTNGEE397 pKa = 4.29GIVYY401 pKa = 8.37FQFDD405 pKa = 3.63GDD407 pKa = 4.0TTDD410 pKa = 4.16RR411 pKa = 11.84GCLQDD416 pKa = 3.72YY417 pKa = 9.32TIMSMFRR424 pKa = 11.84AGALEE429 pKa = 3.75EE430 pKa = 4.22LALGEE435 pKa = 4.38SVVFQLVDD443 pKa = 3.07ARR445 pKa = 11.84TNNVLNYY452 pKa = 9.8VRR454 pKa = 11.84LHH456 pKa = 6.57RR457 pKa = 11.84EE458 pKa = 3.76GFMSAVMPATSTEE471 pKa = 3.6YY472 pKa = 10.72DD473 pKa = 3.33LRR475 pKa = 11.84DD476 pKa = 2.91IYY478 pKa = 10.87LRR480 pKa = 11.84YY481 pKa = 9.08MEE483 pKa = 5.36KK484 pKa = 10.78LPVSNPLPGSQLAEE498 pKa = 3.9LTSQNYY504 pKa = 9.96ALNKK508 pKa = 10.06AADD511 pKa = 3.78ALRR514 pKa = 11.84DD515 pKa = 3.39EE516 pKa = 4.54LMEE519 pKa = 4.23VKK521 pKa = 10.18QAFAGLASSVEE532 pKa = 4.44SEE534 pKa = 4.19LQTSKK539 pKa = 10.97GSKK542 pKa = 10.01LPWRR546 pKa = 11.84RR547 pKa = 11.84QQQKK551 pKa = 9.78QSQQRR556 pKa = 11.84LQGALAASLEE566 pKa = 4.32SLEE569 pKa = 4.44EE570 pKa = 4.07TSSLQAA576 pKa = 4.09

Molecular weight: 63.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KHK3|A0A1L3KHK3_9VIRU Calici_coat domain-containing protein OS=Beihai sipunculid worm virus 5 OX=1922677 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 4.78SANLSISPVYY12 pKa = 10.26QGPLDD17 pKa = 4.46LNSEE21 pKa = 4.39IYY23 pKa = 10.75VGEE26 pKa = 4.1CDD28 pKa = 4.15KK29 pKa = 11.58FGDD32 pKa = 3.39KK33 pKa = 10.19RR34 pKa = 11.84KK35 pKa = 10.1RR36 pKa = 11.84RR37 pKa = 11.84VKK39 pKa = 10.17IKK41 pKa = 10.4NVRR44 pKa = 11.84CKK46 pKa = 10.66QIVHH50 pKa = 5.56TVIFGNTEE58 pKa = 3.58IPVTLACEE66 pKa = 4.17EE67 pKa = 3.96NRR69 pKa = 11.84QAVHH73 pKa = 7.19ANTCPHH79 pKa = 6.3GNEE82 pKa = 3.86TVPRR86 pKa = 11.84LKK88 pKa = 10.51CIAAARR94 pKa = 11.84VFEE97 pKa = 4.32SSRR100 pKa = 11.84LEE102 pKa = 3.91PPPAISRR109 pKa = 11.84FQKK112 pKa = 9.28EE113 pKa = 3.54NCYY116 pKa = 9.73FASQVFRR123 pKa = 11.84LFCFILQCIPSLVTMLPPEE142 pKa = 4.64SGPSVKK148 pKa = 10.28VLEE151 pKa = 4.44SEE153 pKa = 4.23KK154 pKa = 10.78VPLFTRR160 pKa = 11.84YY161 pKa = 9.11HH162 pKa = 6.34LKK164 pKa = 10.73SMPDD168 pKa = 3.55RR169 pKa = 11.84TPMDD173 pKa = 4.16EE174 pKa = 6.1DD175 pKa = 3.12ISTYY179 pKa = 9.15IWCVQPIHH187 pKa = 6.39IWVFEE192 pKa = 4.02LVVAHH197 pKa = 6.41INWLRR202 pKa = 11.84FCRR205 pKa = 11.84FPSVYY210 pKa = 9.33TYY212 pKa = 11.42LSGTCLCSQAPFFICSDD229 pKa = 3.7EE230 pKa = 4.18KK231 pKa = 11.02CRR233 pKa = 11.84RR234 pKa = 11.84NPKK237 pKa = 8.54TDD239 pKa = 3.33FRR241 pKa = 11.84FWPRR245 pKa = 11.84FVPQEE250 pKa = 3.98LSTDD254 pKa = 3.44RR255 pKa = 11.84LDD257 pKa = 4.3EE258 pKa = 4.35LKK260 pKa = 10.47QYY262 pKa = 10.66KK263 pKa = 6.5WTRR266 pKa = 11.84SLRR269 pKa = 11.84AGVWRR274 pKa = 11.84MLSGQRR280 pKa = 11.84RR281 pKa = 11.84FIKK284 pKa = 10.27PMIPSEE290 pKa = 4.15IEE292 pKa = 3.58SSYY295 pKa = 10.7MKK297 pKa = 10.41VIQFIYY303 pKa = 8.32WQSFLLCSYY312 pKa = 10.05GRR314 pKa = 11.84RR315 pKa = 11.84AHH317 pKa = 6.18PHH319 pKa = 4.28NHH321 pKa = 6.13PPVKK325 pKa = 10.48NVMLWRR331 pKa = 11.84TKK333 pKa = 9.4TKK335 pKa = 10.73LVGSFINYY343 pKa = 5.87MWTNFGSLPRR353 pKa = 11.84KK354 pKa = 9.09EE355 pKa = 4.95DD356 pKa = 3.31LVPFYY361 pKa = 11.2RR362 pKa = 11.84MTKK365 pKa = 7.96MMRR368 pKa = 11.84TKK370 pKa = 10.43FYY372 pKa = 11.45AEE374 pKa = 3.85VLAVKK379 pKa = 9.75QKK381 pKa = 10.42KK382 pKa = 9.98GKK384 pKa = 8.44ATEE387 pKa = 4.13SDD389 pKa = 2.72RR390 pKa = 11.84HH391 pKa = 5.92LIRR394 pKa = 11.84RR395 pKa = 11.84SLHH398 pKa = 4.96QIWYY402 pKa = 9.29KK403 pKa = 10.52VLSPSTPNKK412 pKa = 10.04LYY414 pKa = 10.64NDD416 pKa = 4.3RR417 pKa = 11.84ASHH420 pKa = 6.3LAAVLIEE427 pKa = 4.29YY428 pKa = 10.34INRR431 pKa = 11.84LPFLKK436 pKa = 10.71SLGQEE441 pKa = 3.55KK442 pKa = 10.54CFNIVLNFSS451 pKa = 3.54

Molecular weight: 52.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3597

202

2368

899.3

101.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.617 ± 0.702

1.974 ± 0.397

5.366 ± 0.648

6.839 ± 1.01

4.198 ± 0.392

6.144 ± 0.821

2.585 ± 0.292

4.754 ± 0.355

7.145 ± 0.952

8.201 ± 0.542

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.198

4.448 ± 0.55

4.643 ± 0.575

4.865 ± 0.591

5.032 ± 0.601

6.533 ± 0.416

6.339 ± 0.494

6.533 ± 0.385

1.501 ± 0.207

3.67 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski