Cotton leaf curl Gezira virus-[Cameroon]
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0MHN4|C0MHN4_9GEMI Isoform of C0MHN5 Replication-associated protein OS=Cotton leaf curl Gezira virus-[Cameroon] OX=627501 GN=C1 PE=4 SV=1
MM1 pKa = 7.61 GAQKK5 pKa = 10.57 DD6 pKa = 3.74 WQSNTKK12 pKa = 9.54 YY13 pKa = 10.57 GKK15 pKa = 9.12 PVQIKK20 pKa = 10.41 GGIPTIFLCNPGPNSSYY37 pKa = 11.07 KK38 pKa = 10.49 EE39 pKa = 3.93 YY40 pKa = 11.01 LDD42 pKa = 3.86 EE43 pKa = 4.99 EE44 pKa = 4.77 KK45 pKa = 10.73 NAHH48 pKa = 5.77 LKK50 pKa = 9.97 SWALKK55 pKa = 8.81 NATFITLSNPLYY67 pKa = 10.77 SGTNQSPASGGEE79 pKa = 3.96 EE80 pKa = 3.9 EE81 pKa = 5.46 SNQEE85 pKa = 4.0 TQDD88 pKa = 3.25
Molecular weight: 9.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.149
IPC2_protein 5.372
IPC_protein 5.143
Toseland 5.092
ProMoST 4.965
Dawson 5.118
Bjellqvist 5.296
Wikipedia 4.965
Rodwell 5.041
Grimsley 5.016
Solomon 5.105
Lehninger 5.067
Nozaki 5.232
DTASelect 5.321
Thurlkill 5.092
EMBOSS 5.003
Sillero 5.308
Patrickios 3.961
IPC_peptide 5.118
IPC2_peptide 5.308
IPC2.peptide.svr19 5.44
Protein with the highest isoelectric point:
>tr|C0MHN4|C0MHN4_9GEMI Isoform of C0MHN5 Replication-associated protein OS=Cotton leaf curl Gezira virus-[Cameroon] OX=627501 GN=C1 PE=4 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.39 SPGLSSARR32 pKa = 11.84 APTVLVTNKK41 pKa = 9.04 RR42 pKa = 11.84 RR43 pKa = 11.84 AWSNRR48 pKa = 11.84 PNYY51 pKa = 9.5 RR52 pKa = 11.84 KK53 pKa = 9.19 PRR55 pKa = 11.84 MYY57 pKa = 10.9 RR58 pKa = 11.84 MYY60 pKa = 10.57 RR61 pKa = 11.84 SPDD64 pKa = 3.29 VPKK67 pKa = 10.81 GCEE70 pKa = 4.39 GPCKK74 pKa = 9.53 VQSYY78 pKa = 11.07 DD79 pKa = 3.43 MNSVMMM85 pKa = 5.32
Molecular weight: 9.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.3
IPC2_protein 10.028
IPC_protein 11.038
Toseland 11.082
ProMoST 11.125
Dawson 11.14
Bjellqvist 10.965
Wikipedia 11.447
Rodwell 11.228
Grimsley 11.184
Solomon 11.403
Lehninger 11.345
Nozaki 11.067
DTASelect 10.965
Thurlkill 11.082
EMBOSS 11.52
Sillero 11.096
Patrickios 10.965
IPC_peptide 11.403
IPC2_peptide 10.248
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
1030
85
188
128.8
14.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.369 ± 1.118
1.553 ± 0.344
3.981 ± 0.784
4.563 ± 0.73
4.369 ± 0.706
5.146 ± 0.429
3.786 ± 0.8
7.087 ± 1.446
6.117 ± 0.71
8.252 ± 1.517
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.01 ± 0.682
6.796 ± 0.737
4.854 ± 0.757
4.951 ± 0.848
6.505 ± 1.244
8.932 ± 1.205
6.893 ± 0.929
4.272 ± 0.74
1.068 ± 0.206
3.495 ± 0.507
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here